The interaction of dipole modifiers with polyene-sterol complexes.

Recently, we showed that the effect of dipole modifiers (flavonoids and styrylpyridinium dyes) on the conductance of single amphotericin B (AmB) channels in sterol-containing lipid bilayers primarily resulted from changes in the membrane dipole potential. The present study examines the effect of dip...

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Autores principales:	Olga S Ostroumova, Svetlana S Efimova, Evgeny G Chulkov, Ludmila V Schagina
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f4c1224add80488bb5da2c478ead125d
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spelling	oai:doaj.org-article:f4c1224add80488bb5da2c478ead125d2021-11-18T07:04:37ZThe interaction of dipole modifiers with polyene-sterol complexes.1932-620310.1371/journal.pone.0045135https://doaj.org/article/f4c1224add80488bb5da2c478ead125d2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23028805/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Recently, we showed that the effect of dipole modifiers (flavonoids and styrylpyridinium dyes) on the conductance of single amphotericin B (AmB) channels in sterol-containing lipid bilayers primarily resulted from changes in the membrane dipole potential. The present study examines the effect of dipole modifiers on the AmB multi-channel activity. The addition of phloretin to cholesterol-containing membranes leads to a significant increase in the steady-state AmB-induced transmembrane current. Quercetin significantly decreases and RH 421 increases the current through ergosterol-containing bilayers. Other tested flavonoids and styrylpyridinium dyes do not affect the channel-forming activity of AmB independently on the sterol composition of the bilayers. The effects obtained in these trials may instead be attributed to the direct interaction of dipole modifiers with AmB/sterol complexes and not to the effect of dipole potential changes. The presence of double bonds in the Δ7 and Δ22 positions of sterol molecules, the number of conjugated double bonds and amino sugar residues in polyene molecules, and the conformation and adsorption plane of dipole modifiers are important factors impacting this interaction.Olga S OstroumovaSvetlana S EfimovaEvgeny G ChulkovLudmila V SchaginaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 9, p e45135 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Olga S Ostroumova Svetlana S Efimova Evgeny G Chulkov Ludmila V Schagina The interaction of dipole modifiers with polyene-sterol complexes.
description	Recently, we showed that the effect of dipole modifiers (flavonoids and styrylpyridinium dyes) on the conductance of single amphotericin B (AmB) channels in sterol-containing lipid bilayers primarily resulted from changes in the membrane dipole potential. The present study examines the effect of dipole modifiers on the AmB multi-channel activity. The addition of phloretin to cholesterol-containing membranes leads to a significant increase in the steady-state AmB-induced transmembrane current. Quercetin significantly decreases and RH 421 increases the current through ergosterol-containing bilayers. Other tested flavonoids and styrylpyridinium dyes do not affect the channel-forming activity of AmB independently on the sterol composition of the bilayers. The effects obtained in these trials may instead be attributed to the direct interaction of dipole modifiers with AmB/sterol complexes and not to the effect of dipole potential changes. The presence of double bonds in the Δ7 and Δ22 positions of sterol molecules, the number of conjugated double bonds and amino sugar residues in polyene molecules, and the conformation and adsorption plane of dipole modifiers are important factors impacting this interaction.
format	article
author	Olga S Ostroumova Svetlana S Efimova Evgeny G Chulkov Ludmila V Schagina
author_facet	Olga S Ostroumova Svetlana S Efimova Evgeny G Chulkov Ludmila V Schagina
author_sort	Olga S Ostroumova
title	The interaction of dipole modifiers with polyene-sterol complexes.
title_short	The interaction of dipole modifiers with polyene-sterol complexes.
title_full	The interaction of dipole modifiers with polyene-sterol complexes.
title_fullStr	The interaction of dipole modifiers with polyene-sterol complexes.
title_full_unstemmed	The interaction of dipole modifiers with polyene-sterol complexes.
title_sort	interaction of dipole modifiers with polyene-sterol complexes.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/f4c1224add80488bb5da2c478ead125d
work_keys_str_mv	AT olgasostroumova theinteractionofdipolemodifierswithpolyenesterolcomplexes AT svetlanasefimova theinteractionofdipolemodifierswithpolyenesterolcomplexes AT evgenygchulkov theinteractionofdipolemodifierswithpolyenesterolcomplexes AT ludmilavschagina theinteractionofdipolemodifierswithpolyenesterolcomplexes AT olgasostroumova interactionofdipolemodifierswithpolyenesterolcomplexes AT svetlanasefimova interactionofdipolemodifierswithpolyenesterolcomplexes AT evgenygchulkov interactionofdipolemodifierswithpolyenesterolcomplexes AT ludmilavschagina interactionofdipolemodifierswithpolyenesterolcomplexes
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The interaction of dipole modifiers with polyene-sterol complexes.

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