Differences in Three-Dimensional Geometric Recognition by Non-Cancerous and Cancerous Epithelial Cells on Microgroove-Based Topography

Abstract During metastasis, cancer cells are exposed to various three-dimensional microstructures within the body, but the relationship between cancer migration and three-dimensional geometry remain largely unclear. Here, such geometric effects on cancerous cells were investigated by characterizing...

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Autores principales:	Keiichiro Kushiro, Tomohiro Yaginuma, Akihide Ryo, Madoka Takai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f4c59793b3344b6ebd03e92b71e7666e
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spelling	oai:doaj.org-article:f4c59793b3344b6ebd03e92b71e7666e2021-12-02T11:52:18ZDifferences in Three-Dimensional Geometric Recognition by Non-Cancerous and Cancerous Epithelial Cells on Microgroove-Based Topography10.1038/s41598-017-03779-62045-2322https://doaj.org/article/f4c59793b3344b6ebd03e92b71e7666e2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03779-6https://doaj.org/toc/2045-2322Abstract During metastasis, cancer cells are exposed to various three-dimensional microstructures within the body, but the relationship between cancer migration and three-dimensional geometry remain largely unclear. Here, such geometric effects on cancerous cells were investigated by characterizing the motility of various cancer cell types on microgroove-based topographies made of polydimethylsiloxane (PDMS), with particular emphasis on distinguishing cancerous and non-cancerous epithelial cells, as well as understanding the underlying mechanism behind such differences. The 90-degree walls enhanced motility for all cell lines, but the degrees of enhancements were less pronounced for the cancerous cells. Interestingly, while the non-cancerous epithelial cell types conformed to the three-dimensional geometrical cues and migrated along the walls, the cancerous cell types exhibited a unique behavior of climbing upright walls, and this was associated with the inability to form stable, polarized actin cytoskeleton along the walls of the microgrooves. Furthermore, when non-cancerous epithelial cell lines were altered to different levels of polarization capabilities and cancer malignancy or treated with inhibitory drugs, their three-dimensional geometry-dependent motility approached those of cancerous cell lines. Overall, the results suggest that cancerous cells may gradually lose geometrical recognition with increasing cancer malignancy, allowing them to roam freely ignoring three-dimensional geometrical cues during metastasis.Keiichiro KushiroTomohiro YaginumaAkihide RyoMadoka TakaiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Keiichiro Kushiro Tomohiro Yaginuma Akihide Ryo Madoka Takai Differences in Three-Dimensional Geometric Recognition by Non-Cancerous and Cancerous Epithelial Cells on Microgroove-Based Topography
description	Abstract During metastasis, cancer cells are exposed to various three-dimensional microstructures within the body, but the relationship between cancer migration and three-dimensional geometry remain largely unclear. Here, such geometric effects on cancerous cells were investigated by characterizing the motility of various cancer cell types on microgroove-based topographies made of polydimethylsiloxane (PDMS), with particular emphasis on distinguishing cancerous and non-cancerous epithelial cells, as well as understanding the underlying mechanism behind such differences. The 90-degree walls enhanced motility for all cell lines, but the degrees of enhancements were less pronounced for the cancerous cells. Interestingly, while the non-cancerous epithelial cell types conformed to the three-dimensional geometrical cues and migrated along the walls, the cancerous cell types exhibited a unique behavior of climbing upright walls, and this was associated with the inability to form stable, polarized actin cytoskeleton along the walls of the microgrooves. Furthermore, when non-cancerous epithelial cell lines were altered to different levels of polarization capabilities and cancer malignancy or treated with inhibitory drugs, their three-dimensional geometry-dependent motility approached those of cancerous cell lines. Overall, the results suggest that cancerous cells may gradually lose geometrical recognition with increasing cancer malignancy, allowing them to roam freely ignoring three-dimensional geometrical cues during metastasis.
format	article
author	Keiichiro Kushiro Tomohiro Yaginuma Akihide Ryo Madoka Takai
author_facet	Keiichiro Kushiro Tomohiro Yaginuma Akihide Ryo Madoka Takai
author_sort	Keiichiro Kushiro
title	Differences in Three-Dimensional Geometric Recognition by Non-Cancerous and Cancerous Epithelial Cells on Microgroove-Based Topography
title_short	Differences in Three-Dimensional Geometric Recognition by Non-Cancerous and Cancerous Epithelial Cells on Microgroove-Based Topography
title_full	Differences in Three-Dimensional Geometric Recognition by Non-Cancerous and Cancerous Epithelial Cells on Microgroove-Based Topography
title_fullStr	Differences in Three-Dimensional Geometric Recognition by Non-Cancerous and Cancerous Epithelial Cells on Microgroove-Based Topography
title_full_unstemmed	Differences in Three-Dimensional Geometric Recognition by Non-Cancerous and Cancerous Epithelial Cells on Microgroove-Based Topography
title_sort	differences in three-dimensional geometric recognition by non-cancerous and cancerous epithelial cells on microgroove-based topography
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f4c59793b3344b6ebd03e92b71e7666e
work_keys_str_mv	AT keiichirokushiro differencesinthreedimensionalgeometricrecognitionbynoncancerousandcancerousepithelialcellsonmicrogroovebasedtopography AT tomohiroyaginuma differencesinthreedimensionalgeometricrecognitionbynoncancerousandcancerousepithelialcellsonmicrogroovebasedtopography AT akihideryo differencesinthreedimensionalgeometricrecognitionbynoncancerousandcancerousepithelialcellsonmicrogroovebasedtopography AT madokatakai differencesinthreedimensionalgeometricrecognitionbynoncancerousandcancerousepithelialcellsonmicrogroovebasedtopography
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Differences in Three-Dimensional Geometric Recognition by Non-Cancerous and Cancerous Epithelial Cells on Microgroove-Based Topography

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