Large-scale voltage imaging in behaving mice using targeted illumination

Summary: Recent improvements in genetically encoded voltage indicators enabled optical imaging of action potentials and subthreshold transmembrane voltage in vivo. To perform high-speed voltage imaging of many neurons simultaneously over a large anatomical area, widefield microscopy remains an essen...

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Autores principales:	Sheng Xiao, Eric Lowet, Howard J. Gritton, Pierre Fabris, Yangyang Wang, Jack Sherman, Rebecca A. Mount, Hua-an Tseng, Heng-Ye Man, Christoph Straub, Kiryl D. Piatkevich, Edward S. Boyden, Jerome Mertz, Xue Han
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Optical imaging Neuroscience Techniques in neuroscience Science Q
Acceso en línea:	https://doaj.org/article/f4c5cb22516847659db00337a7b75365
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spelling	oai:doaj.org-article:f4c5cb22516847659db00337a7b753652021-11-20T05:09:01ZLarge-scale voltage imaging in behaving mice using targeted illumination2589-004210.1016/j.isci.2021.103263https://doaj.org/article/f4c5cb22516847659db00337a7b753652021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2589004221012323https://doaj.org/toc/2589-0042Summary: Recent improvements in genetically encoded voltage indicators enabled optical imaging of action potentials and subthreshold transmembrane voltage in vivo. To perform high-speed voltage imaging of many neurons simultaneously over a large anatomical area, widefield microscopy remains an essential tool. However, the lack of optical sectioning makes widefield microscopy prone to background cross-contamination. We implemented a digital-micromirror-device-based targeted illumination strategy to restrict illumination to the cells of interest and quantified the resulting improvement both theoretically and experimentally with SomArchon expressing neurons. We found that targeted illumination increased SomArchon signal contrast, decreased photobleaching, and reduced background cross-contamination. With the use of a high-speed, large-area sCMOS camera, we routinely imaged tens of spiking neurons simultaneously over minutes in behaving mice. Thus, the targeted illumination strategy described here offers a simple solution for widefield voltage imaging of many neurons over a large field of view in behaving animals.Sheng XiaoEric LowetHoward J. GrittonPierre FabrisYangyang WangJack ShermanRebecca A. MountHua-an TsengHeng-Ye ManChristoph StraubKiryl D. PiatkevichEdward S. BoydenJerome MertzXue HanElsevierarticleOptical imagingNeuroscienceTechniques in neuroscienceScienceQENiScience, Vol 24, Iss 11, Pp 103263- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Optical imaging Neuroscience Techniques in neuroscience Science Q
spellingShingle	Optical imaging Neuroscience Techniques in neuroscience Science Q Sheng Xiao Eric Lowet Howard J. Gritton Pierre Fabris Yangyang Wang Jack Sherman Rebecca A. Mount Hua-an Tseng Heng-Ye Man Christoph Straub Kiryl D. Piatkevich Edward S. Boyden Jerome Mertz Xue Han Large-scale voltage imaging in behaving mice using targeted illumination
description	Summary: Recent improvements in genetically encoded voltage indicators enabled optical imaging of action potentials and subthreshold transmembrane voltage in vivo. To perform high-speed voltage imaging of many neurons simultaneously over a large anatomical area, widefield microscopy remains an essential tool. However, the lack of optical sectioning makes widefield microscopy prone to background cross-contamination. We implemented a digital-micromirror-device-based targeted illumination strategy to restrict illumination to the cells of interest and quantified the resulting improvement both theoretically and experimentally with SomArchon expressing neurons. We found that targeted illumination increased SomArchon signal contrast, decreased photobleaching, and reduced background cross-contamination. With the use of a high-speed, large-area sCMOS camera, we routinely imaged tens of spiking neurons simultaneously over minutes in behaving mice. Thus, the targeted illumination strategy described here offers a simple solution for widefield voltage imaging of many neurons over a large field of view in behaving animals.
format	article
author	Sheng Xiao Eric Lowet Howard J. Gritton Pierre Fabris Yangyang Wang Jack Sherman Rebecca A. Mount Hua-an Tseng Heng-Ye Man Christoph Straub Kiryl D. Piatkevich Edward S. Boyden Jerome Mertz Xue Han
author_facet	Sheng Xiao Eric Lowet Howard J. Gritton Pierre Fabris Yangyang Wang Jack Sherman Rebecca A. Mount Hua-an Tseng Heng-Ye Man Christoph Straub Kiryl D. Piatkevich Edward S. Boyden Jerome Mertz Xue Han
author_sort	Sheng Xiao
title	Large-scale voltage imaging in behaving mice using targeted illumination
title_short	Large-scale voltage imaging in behaving mice using targeted illumination
title_full	Large-scale voltage imaging in behaving mice using targeted illumination
title_fullStr	Large-scale voltage imaging in behaving mice using targeted illumination
title_full_unstemmed	Large-scale voltage imaging in behaving mice using targeted illumination
title_sort	large-scale voltage imaging in behaving mice using targeted illumination
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/f4c5cb22516847659db00337a7b75365
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Large-scale voltage imaging in behaving mice using targeted illumination

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