Influence of 17β-estradiol on gene expression of Paracoccidioides during mycelia-to-yeast transition.

<h4>Background</h4>Paracoccidioides is the causative agent of paracoccidioidomycosis, a systemic mycosis endemic to Latin America. Infection is initiated by inhalation of conidia (C) or mycelial (M) fragments, which subsequently differentiate into yeast (Y). Epidemiological studies show...

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Autores principales:	Jata Shankar, Thomas D Wu, Karl V Clemons, Jomar P Monteiro, Laurence F Mirels, David A Stevens
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f4cadcd418c949a6a8431b4cb4aaf7e9
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spelling	oai:doaj.org-article:f4cadcd418c949a6a8431b4cb4aaf7e92021-11-18T07:32:21ZInfluence of 17β-estradiol on gene expression of Paracoccidioides during mycelia-to-yeast transition.1932-620310.1371/journal.pone.0028402https://doaj.org/article/f4cadcd418c949a6a8431b4cb4aaf7e92011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22194832/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Paracoccidioides is the causative agent of paracoccidioidomycosis, a systemic mycosis endemic to Latin America. Infection is initiated by inhalation of conidia (C) or mycelial (M) fragments, which subsequently differentiate into yeast (Y). Epidemiological studies show a striking predominance of paracoccidioidomycosis in adult men compared to premenopausal women. In vitro and in vivo studies suggest that the female hormone (17β-estradiol, E(2)) regulates or inhibits M-or-C-to-Y transition. In this study we have profiled transcript expression to understand the molecular mechanism of how E(2) inhibits M-to-Y transition.<h4>Methodology</h4>We assessed temporal gene expression in strain Pb01 in the presence or absence of E(2) at various time points through 9 days of the M-to-Y transition using an 11,000 element random-shear genomic DNA microarray and verified the results using quantitative real time-PCR. E(2)-regulated clones were sequenced to identify genes and biological function.<h4>Principal findings</h4>E(2)-treatment affected gene expression of 550 array elements, with 331 showing up-regulation and 219 showing down-regulation at one or more time points (p≤0.001). Genes with low expression after 4 or 12 h exposure to E(2) belonged to pathways involved in heat shock response (hsp90 and hsp70), energy metabolism, and several retrotransposable elements. Y-related genes, α-1,3-glucan synthase, mannosyltransferase and Y20, demonstrated low or delayed expression in E(2)-treated cultures. Genes potentially involved in signaling, such as palmitoyltransferase (erf2), small GTPase RhoA, phosphatidylinositol-4-kinase, and protein kinase (serine/threonine) showed low expression in the presence of E(2), whereas a gene encoding for an arrestin domain-containing protein showed high expression. Genes related to ubiquitin-mediated protein degradation, and oxidative stress response genes were up-regulated by E(2).<h4>Conclusion</h4>This study characterizes the effect of E(2) at the molecular level on the inhibition of the M-to-Y transition and is indicative that the inhibitory actions of E(2) may be working through signaling genes that regulate dimorphism.Jata ShankarThomas D WuKarl V ClemonsJomar P MonteiroLaurence F MirelsDavid A StevensPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 12, p e28402 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jata Shankar Thomas D Wu Karl V Clemons Jomar P Monteiro Laurence F Mirels David A Stevens Influence of 17β-estradiol on gene expression of Paracoccidioides during mycelia-to-yeast transition.
description	<h4>Background</h4>Paracoccidioides is the causative agent of paracoccidioidomycosis, a systemic mycosis endemic to Latin America. Infection is initiated by inhalation of conidia (C) or mycelial (M) fragments, which subsequently differentiate into yeast (Y). Epidemiological studies show a striking predominance of paracoccidioidomycosis in adult men compared to premenopausal women. In vitro and in vivo studies suggest that the female hormone (17β-estradiol, E(2)) regulates or inhibits M-or-C-to-Y transition. In this study we have profiled transcript expression to understand the molecular mechanism of how E(2) inhibits M-to-Y transition.<h4>Methodology</h4>We assessed temporal gene expression in strain Pb01 in the presence or absence of E(2) at various time points through 9 days of the M-to-Y transition using an 11,000 element random-shear genomic DNA microarray and verified the results using quantitative real time-PCR. E(2)-regulated clones were sequenced to identify genes and biological function.<h4>Principal findings</h4>E(2)-treatment affected gene expression of 550 array elements, with 331 showing up-regulation and 219 showing down-regulation at one or more time points (p≤0.001). Genes with low expression after 4 or 12 h exposure to E(2) belonged to pathways involved in heat shock response (hsp90 and hsp70), energy metabolism, and several retrotransposable elements. Y-related genes, α-1,3-glucan synthase, mannosyltransferase and Y20, demonstrated low or delayed expression in E(2)-treated cultures. Genes potentially involved in signaling, such as palmitoyltransferase (erf2), small GTPase RhoA, phosphatidylinositol-4-kinase, and protein kinase (serine/threonine) showed low expression in the presence of E(2), whereas a gene encoding for an arrestin domain-containing protein showed high expression. Genes related to ubiquitin-mediated protein degradation, and oxidative stress response genes were up-regulated by E(2).<h4>Conclusion</h4>This study characterizes the effect of E(2) at the molecular level on the inhibition of the M-to-Y transition and is indicative that the inhibitory actions of E(2) may be working through signaling genes that regulate dimorphism.
format	article
author	Jata Shankar Thomas D Wu Karl V Clemons Jomar P Monteiro Laurence F Mirels David A Stevens
author_facet	Jata Shankar Thomas D Wu Karl V Clemons Jomar P Monteiro Laurence F Mirels David A Stevens
author_sort	Jata Shankar
title	Influence of 17β-estradiol on gene expression of Paracoccidioides during mycelia-to-yeast transition.
title_short	Influence of 17β-estradiol on gene expression of Paracoccidioides during mycelia-to-yeast transition.
title_full	Influence of 17β-estradiol on gene expression of Paracoccidioides during mycelia-to-yeast transition.
title_fullStr	Influence of 17β-estradiol on gene expression of Paracoccidioides during mycelia-to-yeast transition.
title_full_unstemmed	Influence of 17β-estradiol on gene expression of Paracoccidioides during mycelia-to-yeast transition.
title_sort	influence of 17β-estradiol on gene expression of paracoccidioides during mycelia-to-yeast transition.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/f4cadcd418c949a6a8431b4cb4aaf7e9
work_keys_str_mv	AT jatashankar influenceof17bestradiolongeneexpressionofparacoccidioidesduringmyceliatoyeasttransition AT thomasdwu influenceof17bestradiolongeneexpressionofparacoccidioidesduringmyceliatoyeasttransition AT karlvclemons influenceof17bestradiolongeneexpressionofparacoccidioidesduringmyceliatoyeasttransition AT jomarpmonteiro influenceof17bestradiolongeneexpressionofparacoccidioidesduringmyceliatoyeasttransition AT laurencefmirels influenceof17bestradiolongeneexpressionofparacoccidioidesduringmyceliatoyeasttransition AT davidastevens influenceof17bestradiolongeneexpressionofparacoccidioidesduringmyceliatoyeasttransition
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Influence of 17β-estradiol on gene expression of Paracoccidioides during mycelia-to-yeast transition.

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