A CRISPR-Cas9 delivery system for in vivo screening of genes in the immune system

A CRISPR-Cas9 delivery system for in vivo screening of genes in the immune system

The use of functional genomics in primary immune cells has been limited by inefficient vector delivery and risk of perturbing cell states. Here the authors present CHimeric IMmune Editing (CHIME) for in vivo evaluation of gene function and pooled screening approaches.

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Bibliographic Details
Main Authors: Martin W. LaFleur, Thao H. Nguyen, Matthew A. Coxe, Kathleen B. Yates, Justin D. Trombley, Sarah A. Weiss, Flavian D. Brown, Jacob E. Gillis, Daniel J. Coxe, John G. Doench, W. Nicholas Haining, Arlene H. Sharpe
Format: article
Language:EN
Published: Nature Portfolio 2019
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Science
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Online Access:https://doaj.org/article/f4cc26f0a06e487587662b0cfb8d4512
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Summary:The use of functional genomics in primary immune cells has been limited by inefficient vector delivery and risk of perturbing cell states. Here the authors present CHimeric IMmune Editing (CHIME) for in vivo evaluation of gene function and pooled screening approaches.

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