A CRISPR-Cas9 delivery system for in vivo screening of genes in the immune system

The use of functional genomics in primary immune cells has been limited by inefficient vector delivery and risk of perturbing cell states. Here the authors present CHimeric IMmune Editing (CHIME) for in vivo evaluation of gene function and pooled screening approaches.

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Autores principales:	Martin W. LaFleur, Thao H. Nguyen, Matthew A. Coxe, Kathleen B. Yates, Justin D. Trombley, Sarah A. Weiss, Flavian D. Brown, Jacob E. Gillis, Daniel J. Coxe, John G. Doench, W. Nicholas Haining, Arlene H. Sharpe
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/f4cc26f0a06e487587662b0cfb8d4512
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spelling	oai:doaj.org-article:f4cc26f0a06e487587662b0cfb8d45122021-12-02T15:36:19ZA CRISPR-Cas9 delivery system for in vivo screening of genes in the immune system10.1038/s41467-019-09656-22041-1723https://doaj.org/article/f4cc26f0a06e487587662b0cfb8d45122019-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-09656-2https://doaj.org/toc/2041-1723The use of functional genomics in primary immune cells has been limited by inefficient vector delivery and risk of perturbing cell states. Here the authors present CHimeric IMmune Editing (CHIME) for in vivo evaluation of gene function and pooled screening approaches.Martin W. LaFleurThao H. NguyenMatthew A. CoxeKathleen B. YatesJustin D. TrombleySarah A. WeissFlavian D. BrownJacob E. GillisDaniel J. CoxeJohn G. DoenchW. Nicholas HainingArlene H. SharpeNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Martin W. LaFleur Thao H. Nguyen Matthew A. Coxe Kathleen B. Yates Justin D. Trombley Sarah A. Weiss Flavian D. Brown Jacob E. Gillis Daniel J. Coxe John G. Doench W. Nicholas Haining Arlene H. Sharpe A CRISPR-Cas9 delivery system for in vivo screening of genes in the immune system
description	The use of functional genomics in primary immune cells has been limited by inefficient vector delivery and risk of perturbing cell states. Here the authors present CHimeric IMmune Editing (CHIME) for in vivo evaluation of gene function and pooled screening approaches.
format	article
author	Martin W. LaFleur Thao H. Nguyen Matthew A. Coxe Kathleen B. Yates Justin D. Trombley Sarah A. Weiss Flavian D. Brown Jacob E. Gillis Daniel J. Coxe John G. Doench W. Nicholas Haining Arlene H. Sharpe
author_facet	Martin W. LaFleur Thao H. Nguyen Matthew A. Coxe Kathleen B. Yates Justin D. Trombley Sarah A. Weiss Flavian D. Brown Jacob E. Gillis Daniel J. Coxe John G. Doench W. Nicholas Haining Arlene H. Sharpe
author_sort	Martin W. LaFleur
title	A CRISPR-Cas9 delivery system for in vivo screening of genes in the immune system
title_short	A CRISPR-Cas9 delivery system for in vivo screening of genes in the immune system
title_full	A CRISPR-Cas9 delivery system for in vivo screening of genes in the immune system
title_fullStr	A CRISPR-Cas9 delivery system for in vivo screening of genes in the immune system
title_full_unstemmed	A CRISPR-Cas9 delivery system for in vivo screening of genes in the immune system
title_sort	crispr-cas9 delivery system for in vivo screening of genes in the immune system
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/f4cc26f0a06e487587662b0cfb8d4512
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A CRISPR-Cas9 delivery system for in vivo screening of genes in the immune system

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