Study of the allergic potential of biorpreparate SANEX
Aim. The purpose of our research was to experimentally investigate the allergenicity of SANEX household biomaterials based on enzymes manufactured by Nviron Biosolutions Incorporation, Canada. Material and Methods. The immune toxic effects of SANEX biopreparations were studied: Antiseptic concent...
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| Autores principales: | , , |
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| Formato: | article |
| Lenguaje: | EN UK |
| Publicado: |
Danylo Halytsky Lviv National Medical University
2019
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/f4cd4182304e4bdfa4cd4d38a5cf86bd |
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| Sumario: | Aim. The purpose of our research was to experimentally investigate the allergenicity of SANEX household biomaterials based on enzymes manufactured by Nviron Biosolutions Incorporation, Canada.
Material and Methods. The immune toxic effects of SANEX biopreparations were studied: Antiseptic concentrate and Antitabac in solution. Drug investigations were conducted on the basis of Central Research Laboratory and Laboratory of Industrial Toxicology of Danylo Halytsky Lviv National Medical University. In an experiment, nonlinear white female rats weighing 175-210 g were taken. In experiments sensitization was performed by a single inhalation injection of 0.25 cm3 of the drug. The degree of sensitization was determined by in vivo and in vitro tests. The reliability of the changes received was estimated using Student's t-criterion.
Results and Discussion. Inhalation sensitization with biological preparations caused changes in leukocyte formula in experimental animals (white rats-females). The content of erythrocytes and the mass concentration of hemoglobin of white rats sensitized with preparates Antitabac and Antiodor were significantly reduced. In the leukocyte formula of experimental animals, a significant increase in the content of eosinophils is noted. In in vitro tests, a significant enhancement of the reaction of specific lysis of leukocytes and the reaction of specific agglomeration of leukocytes was revealed at a dilution of 1:10. Among the studied parameters of cellular immunity in experimental animals, no significant changes were found, only in animals sensitized with the Antiodor biological preparation, there was a significant increase in the cytotoxic lymphocyte content (CD8). The value of the immunoregulatory index was significantly decreased in animals sensitized by the biological preparation of Antitabac.
Conclusions. Biopreparations Antitabac in solution and Antiseptic concentrate can lead to the development of anemia in experimental animals with inhalation effects. The investigated biologics have a moderate allergogenic potential. |
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