Study of the allergic potential of biorpreparate SANEX

Aim. The purpose of our research was to experimentally investigate the allergenicity of SANEX household biomaterials based on enzymes manufactured by Nviron Biosolutions Incorporation, Canada. Material and Methods. The immune toxic effects of SANEX biopreparations were studied: Antiseptic concent...

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Autores principales:	V.A. Turkina, O.I. Hrushka, T.A. Alyokhina
Formato:	article
Lenguaje:	EN UK
Publicado:	Danylo Halytsky Lviv National Medical University 2019
Materias:	sensitization, biopreparations, allergopotential Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f4cd4182304e4bdfa4cd4d38a5cf86bd
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spelling	oai:doaj.org-article:f4cd4182304e4bdfa4cd4d38a5cf86bd2021-11-10T19:49:51ZStudy of the allergic potential of biorpreparate SANEX1029-42442415-330310.25040/aml2019.02.064https://doaj.org/article/f4cd4182304e4bdfa4cd4d38a5cf86bd2019-09-01T00:00:00Zhttps://amljournal.com/index.php/journal/article/view/63https://doaj.org/toc/1029-4244https://doaj.org/toc/2415-3303Aim. The purpose of our research was to experimentally investigate the allergenicity of SANEX household biomaterials based on enzymes manufactured by Nviron Biosolutions Incorporation, Canada. Material and Methods. The immune toxic effects of SANEX biopreparations were studied: Antiseptic concentrate and Antitabac in solution. Drug investigations were conducted on the basis of Central Research Laboratory and Laboratory of Industrial Toxicology of Danylo Halytsky Lviv National Medical University. In an experiment, nonlinear white female rats weighing 175-210 g were taken. In experiments sensitization was performed by a single inhalation injection of 0.25 cm3 of the drug. The degree of sensitization was determined by in vivo and in vitro tests. The reliability of the changes received was estimated using Student's t-criterion. Results and Discussion. Inhalation sensitization with biological preparations caused changes in leukocyte formula in experimental animals (white rats-females). The content of erythrocytes and the mass concentration of hemoglobin of white rats sensitized with preparates Antitabac and Antiodor were significantly reduced. In the leukocyte formula of experimental animals, a significant increase in the content of eosinophils is noted. In in vitro tests, a significant enhancement of the reaction of specific lysis of leukocytes and the reaction of specific agglomeration of leukocytes was revealed at a dilution of 1:10. Among the studied parameters of cellular immunity in experimental animals, no significant changes were found, only in animals sensitized with the Antiodor biological preparation, there was a significant increase in the cytotoxic lymphocyte content (CD8). The value of the immunoregulatory index was significantly decreased in animals sensitized by the biological preparation of Antitabac. Conclusions. Biopreparations Antitabac in solution and Antiseptic concentrate can lead to the development of anemia in experimental animals with inhalation effects. The investigated biologics have a moderate allergogenic potential.V.A. TurkinaO.I. HrushkaT.A. AlyokhinaDanylo Halytsky Lviv National Medical Universityarticlesensitization, biopreparations, allergopotentialMedicine (General)R5-920ENUKActa Medica Leopoliensia, Vol 25, Iss 2-3, Pp 64-68 (2019)
institution	DOAJ
collection	DOAJ
language	EN UK
topic	sensitization, biopreparations, allergopotential Medicine (General) R5-920
spellingShingle	sensitization, biopreparations, allergopotential Medicine (General) R5-920 V.A. Turkina O.I. Hrushka T.A. Alyokhina Study of the allergic potential of biorpreparate SANEX
description	Aim. The purpose of our research was to experimentally investigate the allergenicity of SANEX household biomaterials based on enzymes manufactured by Nviron Biosolutions Incorporation, Canada. Material and Methods. The immune toxic effects of SANEX biopreparations were studied: Antiseptic concentrate and Antitabac in solution. Drug investigations were conducted on the basis of Central Research Laboratory and Laboratory of Industrial Toxicology of Danylo Halytsky Lviv National Medical University. In an experiment, nonlinear white female rats weighing 175-210 g were taken. In experiments sensitization was performed by a single inhalation injection of 0.25 cm3 of the drug. The degree of sensitization was determined by in vivo and in vitro tests. The reliability of the changes received was estimated using Student's t-criterion. Results and Discussion. Inhalation sensitization with biological preparations caused changes in leukocyte formula in experimental animals (white rats-females). The content of erythrocytes and the mass concentration of hemoglobin of white rats sensitized with preparates Antitabac and Antiodor were significantly reduced. In the leukocyte formula of experimental animals, a significant increase in the content of eosinophils is noted. In in vitro tests, a significant enhancement of the reaction of specific lysis of leukocytes and the reaction of specific agglomeration of leukocytes was revealed at a dilution of 1:10. Among the studied parameters of cellular immunity in experimental animals, no significant changes were found, only in animals sensitized with the Antiodor biological preparation, there was a significant increase in the cytotoxic lymphocyte content (CD8). The value of the immunoregulatory index was significantly decreased in animals sensitized by the biological preparation of Antitabac. Conclusions. Biopreparations Antitabac in solution and Antiseptic concentrate can lead to the development of anemia in experimental animals with inhalation effects. The investigated biologics have a moderate allergogenic potential.
format	article
author	V.A. Turkina O.I. Hrushka T.A. Alyokhina
author_facet	V.A. Turkina O.I. Hrushka T.A. Alyokhina
author_sort	V.A. Turkina
title	Study of the allergic potential of biorpreparate SANEX
title_short	Study of the allergic potential of biorpreparate SANEX
title_full	Study of the allergic potential of biorpreparate SANEX
title_fullStr	Study of the allergic potential of biorpreparate SANEX
title_full_unstemmed	Study of the allergic potential of biorpreparate SANEX
title_sort	study of the allergic potential of biorpreparate sanex
publisher	Danylo Halytsky Lviv National Medical University
publishDate	2019
url	https://doaj.org/article/f4cd4182304e4bdfa4cd4d38a5cf86bd
work_keys_str_mv	AT vaturkina studyoftheallergicpotentialofbiorpreparatesanex AT oihrushka studyoftheallergicpotentialofbiorpreparatesanex AT taalyokhina studyoftheallergicpotentialofbiorpreparatesanex
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Study of the allergic potential of biorpreparate SANEX

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