Cloning and characterization of TaPP2AbB"-α, a member of the PP2A regulatory subunit in wheat.

Protein phosphatase 2A (PP2A), a major Serine/Threonine protein phosphatase, consists of three subunits; a highly conserved structural subunit A, a catalytic subunit C, and a highly variable regulatory subunit B which determines the substrate specificity. Although the functional mechanism of PP2A in...

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Autores principales:	Dan Liu, Ang Li, Xinguo Mao, Ruilian Jing
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f4ceaab259f940188c70a90dfd5f98ce
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spelling	oai:doaj.org-article:f4ceaab259f940188c70a90dfd5f98ce2021-11-18T08:24:39ZCloning and characterization of TaPP2AbB"-α, a member of the PP2A regulatory subunit in wheat.1932-620310.1371/journal.pone.0094430https://doaj.org/article/f4ceaab259f940188c70a90dfd5f98ce2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24709994/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Protein phosphatase 2A (PP2A), a major Serine/Threonine protein phosphatase, consists of three subunits; a highly conserved structural subunit A, a catalytic subunit C, and a highly variable regulatory subunit B which determines the substrate specificity. Although the functional mechanism of PP2A in signaling transduction in Arabidopsis is known, their physiological roles in wheat remain to be characterized. In this study, we identified a novel regulatory subunit B, TaPP2AbB"-α, in wheat (Triticum aestivum L.). Subcellular localization indicated that TaPP2AbB"-α is located in the cell membrane, cytoplasm and nucleus. It interacts with both TaPP2Aa and TaPP2Ac. Expression pattern analyses revealed that TaPP2AbB"-α is strongly expressed in roots, and responds to NaCl, polyethylene glycol (PEG), cold and abscisic acid (ABA) stresses at the transcription level. Transgenic Arabidopsis plants overexpressing TaPP2AbB"-α developed more lateral roots, especially when treated with mannitol or NaCl. These results suggest that TaPP2AbB"-α, in conjunction with the other two PP2A subunits, is involved in multi-stress response, and positively regulates lateral root development under osmotic stress.Dan LiuAng LiXinguo MaoRuilian JingPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 4, p e94430 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dan Liu Ang Li Xinguo Mao Ruilian Jing Cloning and characterization of TaPP2AbB"-α, a member of the PP2A regulatory subunit in wheat.
description	Protein phosphatase 2A (PP2A), a major Serine/Threonine protein phosphatase, consists of three subunits; a highly conserved structural subunit A, a catalytic subunit C, and a highly variable regulatory subunit B which determines the substrate specificity. Although the functional mechanism of PP2A in signaling transduction in Arabidopsis is known, their physiological roles in wheat remain to be characterized. In this study, we identified a novel regulatory subunit B, TaPP2AbB"-α, in wheat (Triticum aestivum L.). Subcellular localization indicated that TaPP2AbB"-α is located in the cell membrane, cytoplasm and nucleus. It interacts with both TaPP2Aa and TaPP2Ac. Expression pattern analyses revealed that TaPP2AbB"-α is strongly expressed in roots, and responds to NaCl, polyethylene glycol (PEG), cold and abscisic acid (ABA) stresses at the transcription level. Transgenic Arabidopsis plants overexpressing TaPP2AbB"-α developed more lateral roots, especially when treated with mannitol or NaCl. These results suggest that TaPP2AbB"-α, in conjunction with the other two PP2A subunits, is involved in multi-stress response, and positively regulates lateral root development under osmotic stress.
format	article
author	Dan Liu Ang Li Xinguo Mao Ruilian Jing
author_facet	Dan Liu Ang Li Xinguo Mao Ruilian Jing
author_sort	Dan Liu
title	Cloning and characterization of TaPP2AbB"-α, a member of the PP2A regulatory subunit in wheat.
title_short	Cloning and characterization of TaPP2AbB"-α, a member of the PP2A regulatory subunit in wheat.
title_full	Cloning and characterization of TaPP2AbB"-α, a member of the PP2A regulatory subunit in wheat.
title_fullStr	Cloning and characterization of TaPP2AbB"-α, a member of the PP2A regulatory subunit in wheat.
title_full_unstemmed	Cloning and characterization of TaPP2AbB"-α, a member of the PP2A regulatory subunit in wheat.
title_sort	cloning and characterization of tapp2abb"-α, a member of the pp2a regulatory subunit in wheat.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/f4ceaab259f940188c70a90dfd5f98ce
work_keys_str_mv	AT danliu cloningandcharacterizationoftapp2abbaamemberofthepp2aregulatorysubunitinwheat AT angli cloningandcharacterizationoftapp2abbaamemberofthepp2aregulatorysubunitinwheat AT xinguomao cloningandcharacterizationoftapp2abbaamemberofthepp2aregulatorysubunitinwheat AT ruilianjing cloningandcharacterizationoftapp2abbaamemberofthepp2aregulatorysubunitinwheat
_version_	1718421837029834752

Cloning and characterization of TaPP2AbB"-α, a member of the PP2A regulatory subunit in wheat.

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