The novel anti-CRISPR AcrIIA22 relieves DNA torsion in target plasmids and impairs SpyCas9 activity.
To overcome CRISPR-Cas defense systems, many phages and mobile genetic elements (MGEs) encode CRISPR-Cas inhibitors called anti-CRISPRs (Acrs). Nearly all characterized Acrs directly bind Cas proteins to inactivate CRISPR immunity. Here, using functional metagenomic selection, we describe AcrIIA22,...
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Autores principales: | Kevin J Forsberg, Danica T Schmidtke, Rachel Werther, Ruben V Uribe, Deanna Hausman, Morten O A Sommer, Barry L Stoddard, Brett K Kaiser, Harmit S Malik |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Public Library of Science (PLoS)
2021
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Materias: | |
Acceso en línea: | https://doaj.org/article/f4d435c0762349c6924b3c0f2c435c31 |
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