Differential oxidation of protein-tyrosine phosphatases during zebrafish caudal fin regeneration

Abstract Zebrafish have the capacity to regenerate lost tissues and organs. Amputation of the caudal fin results in a rapid, transient increase in H2O2 levels emanating from the wound margin, which is essential for regeneration, because quenching of reactive oxygen species blocks regeneration. Prote...

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Autores principales:	Wei Wu, Alexander James Hale, Simone Lemeer, Jeroen den Hertog
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f4d55fdc896347ac8faa3217761bdfad
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spelling	oai:doaj.org-article:f4d55fdc896347ac8faa3217761bdfad2021-12-02T12:32:00ZDifferential oxidation of protein-tyrosine phosphatases during zebrafish caudal fin regeneration10.1038/s41598-017-07109-82045-2322https://doaj.org/article/f4d55fdc896347ac8faa3217761bdfad2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07109-8https://doaj.org/toc/2045-2322Abstract Zebrafish have the capacity to regenerate lost tissues and organs. Amputation of the caudal fin results in a rapid, transient increase in H2O2 levels emanating from the wound margin, which is essential for regeneration, because quenching of reactive oxygen species blocks regeneration. Protein-tyrosine phosphatases (PTPs) have a central role in cell signalling and are susceptible to oxidation, which results in transient inactivation of their catalytic activity. We hypothesized that PTPs may become oxidized in response to amputation of the caudal fin. Using the oxidized PTP-specific (ox-PTP) antibody and liquid chromatography-mass spectrometry, we identified 33 PTPs in adult zebrafish fin clips of the total of 44 PTPs that can theoretically be detected based on sequence conservation. Of these 33 PTPs, 8 were significantly more oxidized 40 min after caudal fin amputation. Surprisingly, Shp2, one of the PTPs that were oxidized in response to caudal fin amputation, was required for caudal fin regeneration. In contrast, Rptpα, which was not oxidized upon amputation, was dispensable for caudal fin regeneration. Our results demonstrate that PTPs are differentially oxidized in response to caudal fin amputation and that there is a differential requirement for PTPs in regeneration.Wei WuAlexander James HaleSimone LemeerJeroen den HertogNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wei Wu Alexander James Hale Simone Lemeer Jeroen den Hertog Differential oxidation of protein-tyrosine phosphatases during zebrafish caudal fin regeneration
description	Abstract Zebrafish have the capacity to regenerate lost tissues and organs. Amputation of the caudal fin results in a rapid, transient increase in H2O2 levels emanating from the wound margin, which is essential for regeneration, because quenching of reactive oxygen species blocks regeneration. Protein-tyrosine phosphatases (PTPs) have a central role in cell signalling and are susceptible to oxidation, which results in transient inactivation of their catalytic activity. We hypothesized that PTPs may become oxidized in response to amputation of the caudal fin. Using the oxidized PTP-specific (ox-PTP) antibody and liquid chromatography-mass spectrometry, we identified 33 PTPs in adult zebrafish fin clips of the total of 44 PTPs that can theoretically be detected based on sequence conservation. Of these 33 PTPs, 8 were significantly more oxidized 40 min after caudal fin amputation. Surprisingly, Shp2, one of the PTPs that were oxidized in response to caudal fin amputation, was required for caudal fin regeneration. In contrast, Rptpα, which was not oxidized upon amputation, was dispensable for caudal fin regeneration. Our results demonstrate that PTPs are differentially oxidized in response to caudal fin amputation and that there is a differential requirement for PTPs in regeneration.
format	article
author	Wei Wu Alexander James Hale Simone Lemeer Jeroen den Hertog
author_facet	Wei Wu Alexander James Hale Simone Lemeer Jeroen den Hertog
author_sort	Wei Wu
title	Differential oxidation of protein-tyrosine phosphatases during zebrafish caudal fin regeneration
title_short	Differential oxidation of protein-tyrosine phosphatases during zebrafish caudal fin regeneration
title_full	Differential oxidation of protein-tyrosine phosphatases during zebrafish caudal fin regeneration
title_fullStr	Differential oxidation of protein-tyrosine phosphatases during zebrafish caudal fin regeneration
title_full_unstemmed	Differential oxidation of protein-tyrosine phosphatases during zebrafish caudal fin regeneration
title_sort	differential oxidation of protein-tyrosine phosphatases during zebrafish caudal fin regeneration
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f4d55fdc896347ac8faa3217761bdfad
work_keys_str_mv	AT weiwu differentialoxidationofproteintyrosinephosphatasesduringzebrafishcaudalfinregeneration AT alexanderjameshale differentialoxidationofproteintyrosinephosphatasesduringzebrafishcaudalfinregeneration AT simonelemeer differentialoxidationofproteintyrosinephosphatasesduringzebrafishcaudalfinregeneration AT jeroendenhertog differentialoxidationofproteintyrosinephosphatasesduringzebrafishcaudalfinregeneration
_version_	1718394253994885120

Differential oxidation of protein-tyrosine phosphatases during zebrafish caudal fin regeneration

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