Uncoupling of bacterial and terrigenous dissolved organic matter dynamics in decomposition experiments.

The biodegradability of terrigenous dissolved organic matter (tDOM) exported to the sea has a major impact on the global carbon cycle, but our understanding of tDOM bioavailability is fragmentary. In this study, the effects of preparative tDOM isolation on microbial decomposition were investigated i...

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Autores principales:	Daniel P R Herlemann, Marcus Manecki, Christian Meeske, Falk Pollehne, Matthias Labrenz, Detlef Schulz-Bull, Thorsten Dittmar, Klaus Jürgens
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f4e290a7fdc94713839c7cbc9256635d
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spelling	oai:doaj.org-article:f4e290a7fdc94713839c7cbc9256635d2021-11-18T08:24:06ZUncoupling of bacterial and terrigenous dissolved organic matter dynamics in decomposition experiments.1932-620310.1371/journal.pone.0093945https://doaj.org/article/f4e290a7fdc94713839c7cbc9256635d2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24718626/?tool=EBIhttps://doaj.org/toc/1932-6203The biodegradability of terrigenous dissolved organic matter (tDOM) exported to the sea has a major impact on the global carbon cycle, but our understanding of tDOM bioavailability is fragmentary. In this study, the effects of preparative tDOM isolation on microbial decomposition were investigated in incubation experiments consisting of mesocosms containing mesohaline water from the Baltic Sea. Dissolved organic carbon (DOC) consumption, molecular DOM composition, bacterial activities, and shifts in bacterial community structure were compared between mesocosms supplemented with riverine tDOM, either as filtered, particle-free river water or as a concentrate obtained by lyophilization/tangential ultrafiltration, and those containing only Baltic Sea water or river water. As shown using ultra-high-resolution mass spectrometry (15 Tesla Fourier-transform ion cyclotron resonance mass spectrometry, FT-ICR-MS) covering approximately 4600 different DOM compounds, the three DOM preparation protocols resulted in distinct patterns of molecular DOM composition. However, despite DOC losses of 4-16% and considerable bacterial production, there was no significant change in DOM composition during the 28-day experiment. Moreover, tDOM addition affected neither DOC degradation nor bacterial dynamics significantly, regardless of the tDOM preparation. This result suggested that the introduced tDOM was largely not bioavailable, at least on the temporal scale of our experiment, and that the observed bacterial activity and DOC decomposition mainly reflected the degradation of unknown, labile, colloidal and low-molecular weight DOM, both of which escape the analytical window of FT-ICR-MS. In contrast to the different tDOM preparations, the initial bacterial inoculum and batch culture conditions determined bacterial community succession and superseded the effects of tDOM addition. The uncoupling of tDOM and bacterial dynamics suggests that mesohaline bacterial communities cannot efficiently utilize tDOM and that in subarctic estuaries other factors are responsible for the removal of imported tDOM.Daniel P R HerlemannMarcus ManeckiChristian MeeskeFalk PollehneMatthias LabrenzDetlef Schulz-BullThorsten DittmarKlaus JürgensPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 4, p e93945 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daniel P R Herlemann Marcus Manecki Christian Meeske Falk Pollehne Matthias Labrenz Detlef Schulz-Bull Thorsten Dittmar Klaus Jürgens Uncoupling of bacterial and terrigenous dissolved organic matter dynamics in decomposition experiments.
description	The biodegradability of terrigenous dissolved organic matter (tDOM) exported to the sea has a major impact on the global carbon cycle, but our understanding of tDOM bioavailability is fragmentary. In this study, the effects of preparative tDOM isolation on microbial decomposition were investigated in incubation experiments consisting of mesocosms containing mesohaline water from the Baltic Sea. Dissolved organic carbon (DOC) consumption, molecular DOM composition, bacterial activities, and shifts in bacterial community structure were compared between mesocosms supplemented with riverine tDOM, either as filtered, particle-free river water or as a concentrate obtained by lyophilization/tangential ultrafiltration, and those containing only Baltic Sea water or river water. As shown using ultra-high-resolution mass spectrometry (15 Tesla Fourier-transform ion cyclotron resonance mass spectrometry, FT-ICR-MS) covering approximately 4600 different DOM compounds, the three DOM preparation protocols resulted in distinct patterns of molecular DOM composition. However, despite DOC losses of 4-16% and considerable bacterial production, there was no significant change in DOM composition during the 28-day experiment. Moreover, tDOM addition affected neither DOC degradation nor bacterial dynamics significantly, regardless of the tDOM preparation. This result suggested that the introduced tDOM was largely not bioavailable, at least on the temporal scale of our experiment, and that the observed bacterial activity and DOC decomposition mainly reflected the degradation of unknown, labile, colloidal and low-molecular weight DOM, both of which escape the analytical window of FT-ICR-MS. In contrast to the different tDOM preparations, the initial bacterial inoculum and batch culture conditions determined bacterial community succession and superseded the effects of tDOM addition. The uncoupling of tDOM and bacterial dynamics suggests that mesohaline bacterial communities cannot efficiently utilize tDOM and that in subarctic estuaries other factors are responsible for the removal of imported tDOM.
format	article
author	Daniel P R Herlemann Marcus Manecki Christian Meeske Falk Pollehne Matthias Labrenz Detlef Schulz-Bull Thorsten Dittmar Klaus Jürgens
author_facet	Daniel P R Herlemann Marcus Manecki Christian Meeske Falk Pollehne Matthias Labrenz Detlef Schulz-Bull Thorsten Dittmar Klaus Jürgens
author_sort	Daniel P R Herlemann
title	Uncoupling of bacterial and terrigenous dissolved organic matter dynamics in decomposition experiments.
title_short	Uncoupling of bacterial and terrigenous dissolved organic matter dynamics in decomposition experiments.
title_full	Uncoupling of bacterial and terrigenous dissolved organic matter dynamics in decomposition experiments.
title_fullStr	Uncoupling of bacterial and terrigenous dissolved organic matter dynamics in decomposition experiments.
title_full_unstemmed	Uncoupling of bacterial and terrigenous dissolved organic matter dynamics in decomposition experiments.
title_sort	uncoupling of bacterial and terrigenous dissolved organic matter dynamics in decomposition experiments.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/f4e290a7fdc94713839c7cbc9256635d
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Uncoupling of bacterial and terrigenous dissolved organic matter dynamics in decomposition experiments.

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