IL-17A injury to retinal ganglion cells is mediated by retinal Müller cells in diabetic retinopathy

Abstract Diabetic retinopathy (DR), the most common and serious ocular complication, recently has been perceived as a neurovascular inflammatory disease. However, role of adaptive immune inflammation driven by T lymphocytes in DR is not yet well elucidated. Therefore, this study aimed to clarify the...

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Autores principales:	Ao-Wang Qiu, Da-Rui Huang, Bin Li, Yuan Fang, Wei-Wei Zhang, Qing-Huai Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Publishing Group 2021
Materias:	Cytology QH573-671
Acceso en línea:	https://doaj.org/article/f4e2e18bcf584abb8e8bff654c18014f
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spelling	oai:doaj.org-article:f4e2e18bcf584abb8e8bff654c18014f2021-11-14T12:06:47ZIL-17A injury to retinal ganglion cells is mediated by retinal Müller cells in diabetic retinopathy10.1038/s41419-021-04350-y2041-4889https://doaj.org/article/f4e2e18bcf584abb8e8bff654c18014f2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41419-021-04350-yhttps://doaj.org/toc/2041-4889Abstract Diabetic retinopathy (DR), the most common and serious ocular complication, recently has been perceived as a neurovascular inflammatory disease. However, role of adaptive immune inflammation driven by T lymphocytes in DR is not yet well elucidated. Therefore, this study aimed to clarify the role of interleukin (IL)-17A, a proinflammatory cytokine mainly produced by T lymphocytes, in retinal pathophysiology particularly in retinal neuronal death during DR process. Ins2Akita (Akita) diabetic mice 12 weeks after the onset of diabetes were used as a DR model. IL-17A-deficient diabetic mice were obtained by hybridization of IL-17A-knockout (IL-17A-KO) mouse with Akita mouse. Primarily cultured retinal Müller cells (RMCs) and retinal ganglion cells (RGCs) were treated with IL-17A in high-glucose (HG) condition. A transwell coculture of RGCs and RMCs whose IL-17 receptor A (IL-17RA) gene had been silenced with IL-17RA-shRNA was exposed to IL-17A in HG condition and the cocultured RGCs were assessed on their survival. Diabetic mice manifested increased retinal microvascular lesions, RMC activation and dysfunction, as well as RGC apoptosis. IL-17A-KO diabetic mice showed reduced retinal microvascular impairments, RMC abnormalities, and RGC apoptosis compared with diabetic mice. RMCs expressed IL-17RA. IL-17A exacerbated HG-induced RMC activation and dysfunction in vitro and silencing IL-17RA gene in RMCs abolished the IL-17A deleterious effects. In contrast, RGCs did not express IL-17RA and IL-17A did not further alter HG-induced RGC death. Notably, IL-17A aggravated HG-induced RGC death in the presence of intact RMCs but not in the presence of RMCs in which IL-17RA gene had been knocked down. These findings establish that IL-17A is actively involved in DR pathophysiology and particularly by RMC mediation it promotes RGC death. Collectively, we propose that antagonizing IL-17RA on RMCs may prevent retinal neuronal death and thereby slow down DR progression.Ao-Wang QiuDa-Rui HuangBin LiYuan FangWei-Wei ZhangQing-Huai LiuNature Publishing GrouparticleCytologyQH573-671ENCell Death and Disease, Vol 12, Iss 11, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cytology QH573-671
spellingShingle	Cytology QH573-671 Ao-Wang Qiu Da-Rui Huang Bin Li Yuan Fang Wei-Wei Zhang Qing-Huai Liu IL-17A injury to retinal ganglion cells is mediated by retinal Müller cells in diabetic retinopathy
description	Abstract Diabetic retinopathy (DR), the most common and serious ocular complication, recently has been perceived as a neurovascular inflammatory disease. However, role of adaptive immune inflammation driven by T lymphocytes in DR is not yet well elucidated. Therefore, this study aimed to clarify the role of interleukin (IL)-17A, a proinflammatory cytokine mainly produced by T lymphocytes, in retinal pathophysiology particularly in retinal neuronal death during DR process. Ins2Akita (Akita) diabetic mice 12 weeks after the onset of diabetes were used as a DR model. IL-17A-deficient diabetic mice were obtained by hybridization of IL-17A-knockout (IL-17A-KO) mouse with Akita mouse. Primarily cultured retinal Müller cells (RMCs) and retinal ganglion cells (RGCs) were treated with IL-17A in high-glucose (HG) condition. A transwell coculture of RGCs and RMCs whose IL-17 receptor A (IL-17RA) gene had been silenced with IL-17RA-shRNA was exposed to IL-17A in HG condition and the cocultured RGCs were assessed on their survival. Diabetic mice manifested increased retinal microvascular lesions, RMC activation and dysfunction, as well as RGC apoptosis. IL-17A-KO diabetic mice showed reduced retinal microvascular impairments, RMC abnormalities, and RGC apoptosis compared with diabetic mice. RMCs expressed IL-17RA. IL-17A exacerbated HG-induced RMC activation and dysfunction in vitro and silencing IL-17RA gene in RMCs abolished the IL-17A deleterious effects. In contrast, RGCs did not express IL-17RA and IL-17A did not further alter HG-induced RGC death. Notably, IL-17A aggravated HG-induced RGC death in the presence of intact RMCs but not in the presence of RMCs in which IL-17RA gene had been knocked down. These findings establish that IL-17A is actively involved in DR pathophysiology and particularly by RMC mediation it promotes RGC death. Collectively, we propose that antagonizing IL-17RA on RMCs may prevent retinal neuronal death and thereby slow down DR progression.
format	article
author	Ao-Wang Qiu Da-Rui Huang Bin Li Yuan Fang Wei-Wei Zhang Qing-Huai Liu
author_facet	Ao-Wang Qiu Da-Rui Huang Bin Li Yuan Fang Wei-Wei Zhang Qing-Huai Liu
author_sort	Ao-Wang Qiu
title	IL-17A injury to retinal ganglion cells is mediated by retinal Müller cells in diabetic retinopathy
title_short	IL-17A injury to retinal ganglion cells is mediated by retinal Müller cells in diabetic retinopathy
title_full	IL-17A injury to retinal ganglion cells is mediated by retinal Müller cells in diabetic retinopathy
title_fullStr	IL-17A injury to retinal ganglion cells is mediated by retinal Müller cells in diabetic retinopathy
title_full_unstemmed	IL-17A injury to retinal ganglion cells is mediated by retinal Müller cells in diabetic retinopathy
title_sort	il-17a injury to retinal ganglion cells is mediated by retinal müller cells in diabetic retinopathy
publisher	Nature Publishing Group
publishDate	2021
url	https://doaj.org/article/f4e2e18bcf584abb8e8bff654c18014f
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IL-17A injury to retinal ganglion cells is mediated by retinal Müller cells in diabetic retinopathy

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