Total phenolic and flavonoid contents, antioxidant, antidiabetic and antiplasmodial activities of Garcinia forbesii King: A correlation study

Garcinia forbesii King belongs to Clusiaceae is a source of secondary metabolites especially xanthones with various biological activities. G. forbesii King is also known for its empirical use for malaria and diabetes. This study investigated the total phenolic and flavonoid contents, in vitro antiox...

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Autores principales:	Johanis Wairata, Arif Fadlan, Adi Setyo Purnomo, Muhammad Taher, Taslim Ersam
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Garcinia forbesii King Antidiabetic Antioxidant Flavonoids Phenolics Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/f4e3622bd7bc4bc492ab06b4ae556ec6
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spelling	oai:doaj.org-article:f4e3622bd7bc4bc492ab06b4ae556ec62021-11-26T04:26:10ZTotal phenolic and flavonoid contents, antioxidant, antidiabetic and antiplasmodial activities of Garcinia forbesii King: A correlation study1878-535210.1016/j.arabjc.2021.103541https://doaj.org/article/f4e3622bd7bc4bc492ab06b4ae556ec62022-02-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1878535221005566https://doaj.org/toc/1878-5352Garcinia forbesii King belongs to Clusiaceae is a source of secondary metabolites especially xanthones with various biological activities. G. forbesii King is also known for its empirical use for malaria and diabetes. This study investigated the total phenolic and flavonoid contents, in vitro antioxidant, antidiabetic and antiplasmodial activities of four extracts attained from the stem bark of G. forbesii King. The total phenolic and flavonoid contents were determined by spectrophotometric methods and antioxidant activity was evaluated by DPPH, ABTS, FRAP assays. In vitro antidiabetic activity was assessed by α-glucosidase and α-amylase assays and antiplasmodial activity was studied against chloroquine sensitive Plasmodium falciparum strain 3D7. The highest value of total phenolic (187.37 ± 0.06 mg GAE/g) and flavonoid (35.97 ± 0.02 mg QE/g) contents were recorded in n-hexane and methanolic extracts. n-Hexane extract showed the highest DPPH activity with IC50 of 8.12 ± 0.02 μg/mL. Ethyl acetate extract exhibited better scavenging ability for ABTS with IC50 of 3.88 ± 0.04 μg/mL. The FRAP assay showed better activity in methanol extract with an inhibition value of 73.68 ± 3.66 µM Fe2+/g. The strong inhibition against α-glucosidase and α-amylase were displayed by dichloromethane extract with IC50 of 35.13 ± 2.01 μg/mL and 4.83 ± 0.20 μg/mL. n-Hexane and methanol extracts showed significant antiplasmodial activity with IC50 of 0.23 ± 0.01 μg/mL and 0.73 ± 0.01 μg/mL, respectively. The correlation analysis indicated a positive relationship of total phenolic and flavonoid contents with antiplasmodial activity. The results revealed that n-hexane and methanol extracts could be used as a potential natural antiplasmodial, while dichloromethane extract is a promising natural antidiabetic.Johanis WairataArif FadlanAdi Setyo PurnomoMuhammad TaherTaslim ErsamElsevierarticleGarcinia forbesii KingAntidiabeticAntioxidantFlavonoidsPhenolicsChemistryQD1-999ENArabian Journal of Chemistry, Vol 15, Iss 2, Pp 103541- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Garcinia forbesii King Antidiabetic Antioxidant Flavonoids Phenolics Chemistry QD1-999
spellingShingle	Garcinia forbesii King Antidiabetic Antioxidant Flavonoids Phenolics Chemistry QD1-999 Johanis Wairata Arif Fadlan Adi Setyo Purnomo Muhammad Taher Taslim Ersam Total phenolic and flavonoid contents, antioxidant, antidiabetic and antiplasmodial activities of Garcinia forbesii King: A correlation study
description	Garcinia forbesii King belongs to Clusiaceae is a source of secondary metabolites especially xanthones with various biological activities. G. forbesii King is also known for its empirical use for malaria and diabetes. This study investigated the total phenolic and flavonoid contents, in vitro antioxidant, antidiabetic and antiplasmodial activities of four extracts attained from the stem bark of G. forbesii King. The total phenolic and flavonoid contents were determined by spectrophotometric methods and antioxidant activity was evaluated by DPPH, ABTS, FRAP assays. In vitro antidiabetic activity was assessed by α-glucosidase and α-amylase assays and antiplasmodial activity was studied against chloroquine sensitive Plasmodium falciparum strain 3D7. The highest value of total phenolic (187.37 ± 0.06 mg GAE/g) and flavonoid (35.97 ± 0.02 mg QE/g) contents were recorded in n-hexane and methanolic extracts. n-Hexane extract showed the highest DPPH activity with IC50 of 8.12 ± 0.02 μg/mL. Ethyl acetate extract exhibited better scavenging ability for ABTS with IC50 of 3.88 ± 0.04 μg/mL. The FRAP assay showed better activity in methanol extract with an inhibition value of 73.68 ± 3.66 µM Fe2+/g. The strong inhibition against α-glucosidase and α-amylase were displayed by dichloromethane extract with IC50 of 35.13 ± 2.01 μg/mL and 4.83 ± 0.20 μg/mL. n-Hexane and methanol extracts showed significant antiplasmodial activity with IC50 of 0.23 ± 0.01 μg/mL and 0.73 ± 0.01 μg/mL, respectively. The correlation analysis indicated a positive relationship of total phenolic and flavonoid contents with antiplasmodial activity. The results revealed that n-hexane and methanol extracts could be used as a potential natural antiplasmodial, while dichloromethane extract is a promising natural antidiabetic.
format	article
author	Johanis Wairata Arif Fadlan Adi Setyo Purnomo Muhammad Taher Taslim Ersam
author_facet	Johanis Wairata Arif Fadlan Adi Setyo Purnomo Muhammad Taher Taslim Ersam
author_sort	Johanis Wairata
title	Total phenolic and flavonoid contents, antioxidant, antidiabetic and antiplasmodial activities of Garcinia forbesii King: A correlation study
title_short	Total phenolic and flavonoid contents, antioxidant, antidiabetic and antiplasmodial activities of Garcinia forbesii King: A correlation study
title_full	Total phenolic and flavonoid contents, antioxidant, antidiabetic and antiplasmodial activities of Garcinia forbesii King: A correlation study
title_fullStr	Total phenolic and flavonoid contents, antioxidant, antidiabetic and antiplasmodial activities of Garcinia forbesii King: A correlation study
title_full_unstemmed	Total phenolic and flavonoid contents, antioxidant, antidiabetic and antiplasmodial activities of Garcinia forbesii King: A correlation study
title_sort	total phenolic and flavonoid contents, antioxidant, antidiabetic and antiplasmodial activities of garcinia forbesii king: a correlation study
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/f4e3622bd7bc4bc492ab06b4ae556ec6
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Total phenolic and flavonoid contents, antioxidant, antidiabetic and antiplasmodial activities of Garcinia forbesii King: A correlation study

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