In vitro culture of freshly isolated Trypanosoma brucei brucei bloodstream forms results in gene copy-number changes.

Most researchers who study unicellular eukaryotes work with an extremely limited number of laboratory-adapted isolates that were obtained from the field decades ago, but the effects of passage in laboratory rodents, and adaptation to in vitro culture, have been little studied. For example, the vast...

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Autores principales:	Julius Mulindwa, Geofrey Ssentamu, Enock Matovu, Kevin Kamanyi Marucha, Francisco Aresta-Branco, Claudia Helbig, Christine Clayton
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/f4e45a8c3db2463794bf3f4118147413
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spelling	oai:doaj.org-article:f4e45a8c3db2463794bf3f41181474132021-12-02T20:24:08ZIn vitro culture of freshly isolated Trypanosoma brucei brucei bloodstream forms results in gene copy-number changes.1935-27271935-273510.1371/journal.pntd.0009738https://doaj.org/article/f4e45a8c3db2463794bf3f41181474132021-09-01T00:00:00Zhttps://doi.org/10.1371/journal.pntd.0009738https://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735Most researchers who study unicellular eukaryotes work with an extremely limited number of laboratory-adapted isolates that were obtained from the field decades ago, but the effects of passage in laboratory rodents, and adaptation to in vitro culture, have been little studied. For example, the vast majority of studies of Trypanosoma brucei biology have concentrated on just two strains, Lister 427 and EATRO1125, which were taken from the field over half a century ago and have since have undergone innumerable passages in rodents and culture. We here describe two new Trypanosoma brucei brucei strains. MAK65 and MAK98, which have undergone only 3 rodent passages since isolation from Ugandan cattle. High-coverage sequencing revealed that adaptation of the parasites to culture was accompanied by changes in gene copy numbers. T. brucei has so far been considered to be uniformly diploid, but we also found trisomy of chromosome 5 not only in one Lister 427 culture, but also in the MAK98 field isolate. Trisomy of chromosome 6, and increased copies of other chromosome segments, were also seen in established cultured lines. The two new T. brucei strains should be useful to researchers interested in trypanosome differentiation and pathogenicity. Initial results suggested that the two strains have differing infection patterns in rodents. MAK65 is uniformly diploid and grew more reproducibly in bloodstream-form culture than MAK98.Julius MulindwaGeofrey SsentamuEnock MatovuKevin Kamanyi MaruchaFrancisco Aresta-BrancoClaudia HelbigChristine ClaytonPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 15, Iss 9, p e0009738 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Julius Mulindwa Geofrey Ssentamu Enock Matovu Kevin Kamanyi Marucha Francisco Aresta-Branco Claudia Helbig Christine Clayton In vitro culture of freshly isolated Trypanosoma brucei brucei bloodstream forms results in gene copy-number changes.
description	Most researchers who study unicellular eukaryotes work with an extremely limited number of laboratory-adapted isolates that were obtained from the field decades ago, but the effects of passage in laboratory rodents, and adaptation to in vitro culture, have been little studied. For example, the vast majority of studies of Trypanosoma brucei biology have concentrated on just two strains, Lister 427 and EATRO1125, which were taken from the field over half a century ago and have since have undergone innumerable passages in rodents and culture. We here describe two new Trypanosoma brucei brucei strains. MAK65 and MAK98, which have undergone only 3 rodent passages since isolation from Ugandan cattle. High-coverage sequencing revealed that adaptation of the parasites to culture was accompanied by changes in gene copy numbers. T. brucei has so far been considered to be uniformly diploid, but we also found trisomy of chromosome 5 not only in one Lister 427 culture, but also in the MAK98 field isolate. Trisomy of chromosome 6, and increased copies of other chromosome segments, were also seen in established cultured lines. The two new T. brucei strains should be useful to researchers interested in trypanosome differentiation and pathogenicity. Initial results suggested that the two strains have differing infection patterns in rodents. MAK65 is uniformly diploid and grew more reproducibly in bloodstream-form culture than MAK98.
format	article
author	Julius Mulindwa Geofrey Ssentamu Enock Matovu Kevin Kamanyi Marucha Francisco Aresta-Branco Claudia Helbig Christine Clayton
author_facet	Julius Mulindwa Geofrey Ssentamu Enock Matovu Kevin Kamanyi Marucha Francisco Aresta-Branco Claudia Helbig Christine Clayton
author_sort	Julius Mulindwa
title	In vitro culture of freshly isolated Trypanosoma brucei brucei bloodstream forms results in gene copy-number changes.
title_short	In vitro culture of freshly isolated Trypanosoma brucei brucei bloodstream forms results in gene copy-number changes.
title_full	In vitro culture of freshly isolated Trypanosoma brucei brucei bloodstream forms results in gene copy-number changes.
title_fullStr	In vitro culture of freshly isolated Trypanosoma brucei brucei bloodstream forms results in gene copy-number changes.
title_full_unstemmed	In vitro culture of freshly isolated Trypanosoma brucei brucei bloodstream forms results in gene copy-number changes.
title_sort	in vitro culture of freshly isolated trypanosoma brucei brucei bloodstream forms results in gene copy-number changes.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/f4e45a8c3db2463794bf3f4118147413
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In vitro culture of freshly isolated Trypanosoma brucei brucei bloodstream forms results in gene copy-number changes.

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