Chronic ethanol exposure during adolescence in rats induces motor impairments and cerebral cortex damage associated with oxidative stress.
Binge drinking is common among adolescents, and this type of ethanol exposure may lead to long-term nervous system damage. In the current study, we evaluated motor performance and tissue alterations in the cerebral cortex of rats subjected to intermittent intoxication with ethanol from adolescence t...
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2014
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oai:doaj.org-article:f4ebbf5b7f0a466f91730394e69b13c42021-11-11T08:21:29ZChronic ethanol exposure during adolescence in rats induces motor impairments and cerebral cortex damage associated with oxidative stress.1932-620310.1371/journal.pone.0101074https://doaj.org/article/f4ebbf5b7f0a466f91730394e69b13c42014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24967633/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Binge drinking is common among adolescents, and this type of ethanol exposure may lead to long-term nervous system damage. In the current study, we evaluated motor performance and tissue alterations in the cerebral cortex of rats subjected to intermittent intoxication with ethanol from adolescence to adulthood. Adolescent male Wistar rats (35 days old) were treated with distilled water or ethanol (6.5 g/kg/day, 22.5% w/v) during 55 days by gavage to complete 90 days of age. The open field, inclined plane and the rotarod tests were used to assess the spontaneous locomotor activity and motor coordination performance in adult animals. Following completion of behavioral tests, half of animals were submitted to immunohistochemical evaluation of NeuN (marker of neuronal bodies), GFAP (a marker of astrocytes) and Iba1 (microglia marker) in the cerebral cortex while the other half of the animals were subjected to analysis of oxidative stress markers by biochemical assays. Chronic ethanol intoxication in rats from adolescence to adulthood induced significant motor deficits including impaired spontaneous locomotion, coordination and muscle strength. These behavioral impairments were accompanied by marked changes in all cellular populations evaluated as well as increased levels of nitrite and lipid peroxidation in the cerebral cortex. These findings indicate that continuous ethanol intoxication from adolescence to adulthood is able to provide neurobehavioral and neurodegenerative damage to cerebral cortex.Francisco Bruno TeixeiraLuana Nazaré da Silva SantanaFernando Romualdo BezerraSabrina De CarvalhoEnéas Andrade Fontes-JúniorRui Daniel PredigerMaria Elena Crespo-LópezCristiane Socorro Ferraz MaiaRafael Rodrigues LimaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e101074 (2014) |
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Medicine R Science Q |
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Medicine R Science Q Francisco Bruno Teixeira Luana Nazaré da Silva Santana Fernando Romualdo Bezerra Sabrina De Carvalho Enéas Andrade Fontes-Júnior Rui Daniel Prediger Maria Elena Crespo-López Cristiane Socorro Ferraz Maia Rafael Rodrigues Lima Chronic ethanol exposure during adolescence in rats induces motor impairments and cerebral cortex damage associated with oxidative stress. |
| description |
Binge drinking is common among adolescents, and this type of ethanol exposure may lead to long-term nervous system damage. In the current study, we evaluated motor performance and tissue alterations in the cerebral cortex of rats subjected to intermittent intoxication with ethanol from adolescence to adulthood. Adolescent male Wistar rats (35 days old) were treated with distilled water or ethanol (6.5 g/kg/day, 22.5% w/v) during 55 days by gavage to complete 90 days of age. The open field, inclined plane and the rotarod tests were used to assess the spontaneous locomotor activity and motor coordination performance in adult animals. Following completion of behavioral tests, half of animals were submitted to immunohistochemical evaluation of NeuN (marker of neuronal bodies), GFAP (a marker of astrocytes) and Iba1 (microglia marker) in the cerebral cortex while the other half of the animals were subjected to analysis of oxidative stress markers by biochemical assays. Chronic ethanol intoxication in rats from adolescence to adulthood induced significant motor deficits including impaired spontaneous locomotion, coordination and muscle strength. These behavioral impairments were accompanied by marked changes in all cellular populations evaluated as well as increased levels of nitrite and lipid peroxidation in the cerebral cortex. These findings indicate that continuous ethanol intoxication from adolescence to adulthood is able to provide neurobehavioral and neurodegenerative damage to cerebral cortex. |
| format |
article |
| author |
Francisco Bruno Teixeira Luana Nazaré da Silva Santana Fernando Romualdo Bezerra Sabrina De Carvalho Enéas Andrade Fontes-Júnior Rui Daniel Prediger Maria Elena Crespo-López Cristiane Socorro Ferraz Maia Rafael Rodrigues Lima |
| author_facet |
Francisco Bruno Teixeira Luana Nazaré da Silva Santana Fernando Romualdo Bezerra Sabrina De Carvalho Enéas Andrade Fontes-Júnior Rui Daniel Prediger Maria Elena Crespo-López Cristiane Socorro Ferraz Maia Rafael Rodrigues Lima |
| author_sort |
Francisco Bruno Teixeira |
| title |
Chronic ethanol exposure during adolescence in rats induces motor impairments and cerebral cortex damage associated with oxidative stress. |
| title_short |
Chronic ethanol exposure during adolescence in rats induces motor impairments and cerebral cortex damage associated with oxidative stress. |
| title_full |
Chronic ethanol exposure during adolescence in rats induces motor impairments and cerebral cortex damage associated with oxidative stress. |
| title_fullStr |
Chronic ethanol exposure during adolescence in rats induces motor impairments and cerebral cortex damage associated with oxidative stress. |
| title_full_unstemmed |
Chronic ethanol exposure during adolescence in rats induces motor impairments and cerebral cortex damage associated with oxidative stress. |
| title_sort |
chronic ethanol exposure during adolescence in rats induces motor impairments and cerebral cortex damage associated with oxidative stress. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/f4ebbf5b7f0a466f91730394e69b13c4 |
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