DNA degradation in human teeth exposed to thermal stress

Abstract Human identification from burned remains poses a challenge to forensic laboratories, and DNA profiling is widely used for this purpose. Our aim was to evaluate the effect of temperature on DNA degradation in human teeth. Thirty teeth were exposed to temperatures of 100, 200, or 400 °C for 6...

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Autores principales:	Diego Lozano-Peral, Leticia Rubio, Ignacio Santos, María Jesús Gaitán, Enrique Viguera, Stella Martín-de-las-Heras
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f4ee884ee8ca4622a640bdacc2625601
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spelling	oai:doaj.org-article:f4ee884ee8ca4622a640bdacc26256012021-12-02T17:34:48ZDNA degradation in human teeth exposed to thermal stress10.1038/s41598-021-91505-82045-2322https://doaj.org/article/f4ee884ee8ca4622a640bdacc26256012021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91505-8https://doaj.org/toc/2045-2322Abstract Human identification from burned remains poses a challenge to forensic laboratories, and DNA profiling is widely used for this purpose. Our aim was to evaluate the effect of temperature on DNA degradation in human teeth. Thirty teeth were exposed to temperatures of 100, 200, or 400 °C for 60 min. DNA was quantified by Real-Time qPCR (Quantifiler Human DNA Quantification Kit) and fluorescence spectroscopy (Qubit 3.0 Fluorometer). DNA degradation was evaluated by using STR markers (AmpFLSTR Identifiler Plus PCR Amplification Kit) to determine the allele and locus dropout, inter-locus balance, and degradation slope (observed (Oa) to expected (Ea) locus peak height ratio against the molecular weight). Most of the genomic DNA was degraded between 100 °C and 200 °C. At 100 °C, locus dropout ratios showed significant differences between the largest loci (FGA, D7S820, D18S51, D16S539, D2S1338 and CSF1PO) and amelogenin. Inter-locus balance values significantly differed between all dye channels except between NED and PET. The dropout ratio between D18S51 (NED) and amelogenin (PET) can be recommended for the evaluation of DNA degradation. The Oa/Ea regression model can predict locus peak heights in DNA degradation (R2 = 0.7881). These findings may be useful to assess the reliability of DNA typing for human identification in teeth subjected to prolonged incineration.Diego Lozano-PeralLeticia RubioIgnacio SantosMaría Jesús GaitánEnrique VigueraStella Martín-de-las-HerasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Diego Lozano-Peral Leticia Rubio Ignacio Santos María Jesús Gaitán Enrique Viguera Stella Martín-de-las-Heras DNA degradation in human teeth exposed to thermal stress
description	Abstract Human identification from burned remains poses a challenge to forensic laboratories, and DNA profiling is widely used for this purpose. Our aim was to evaluate the effect of temperature on DNA degradation in human teeth. Thirty teeth were exposed to temperatures of 100, 200, or 400 °C for 60 min. DNA was quantified by Real-Time qPCR (Quantifiler Human DNA Quantification Kit) and fluorescence spectroscopy (Qubit 3.0 Fluorometer). DNA degradation was evaluated by using STR markers (AmpFLSTR Identifiler Plus PCR Amplification Kit) to determine the allele and locus dropout, inter-locus balance, and degradation slope (observed (Oa) to expected (Ea) locus peak height ratio against the molecular weight). Most of the genomic DNA was degraded between 100 °C and 200 °C. At 100 °C, locus dropout ratios showed significant differences between the largest loci (FGA, D7S820, D18S51, D16S539, D2S1338 and CSF1PO) and amelogenin. Inter-locus balance values significantly differed between all dye channels except between NED and PET. The dropout ratio between D18S51 (NED) and amelogenin (PET) can be recommended for the evaluation of DNA degradation. The Oa/Ea regression model can predict locus peak heights in DNA degradation (R2 = 0.7881). These findings may be useful to assess the reliability of DNA typing for human identification in teeth subjected to prolonged incineration.
format	article
author	Diego Lozano-Peral Leticia Rubio Ignacio Santos María Jesús Gaitán Enrique Viguera Stella Martín-de-las-Heras
author_facet	Diego Lozano-Peral Leticia Rubio Ignacio Santos María Jesús Gaitán Enrique Viguera Stella Martín-de-las-Heras
author_sort	Diego Lozano-Peral
title	DNA degradation in human teeth exposed to thermal stress
title_short	DNA degradation in human teeth exposed to thermal stress
title_full	DNA degradation in human teeth exposed to thermal stress
title_fullStr	DNA degradation in human teeth exposed to thermal stress
title_full_unstemmed	DNA degradation in human teeth exposed to thermal stress
title_sort	dna degradation in human teeth exposed to thermal stress
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f4ee884ee8ca4622a640bdacc2625601
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DNA degradation in human teeth exposed to thermal stress

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