Pathological effect of arterial ischaemia and venous congestion on rat testes

Abstract Many studies on various organs have concluded that venous congestion (VC) causes severe organ dysfunction with elevation of oxidative stress relative to that of arterial ischaemia (AI). However, a comparison of the pathological effects of AI and VC on the testes has not been conducted. In t...

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Autores principales:	Shuichi Hirai, Naoyuki Hatayama, Munekazu Naito, Kenta Nagahori, Shinichi Kawata, Shogo Hayashi, Ning Qu, Hayato Terayama, Sunao Shoji, Masahiro Itoh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f4ef5fff811042e0ac2e675e7b9f0122
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spelling	oai:doaj.org-article:f4ef5fff811042e0ac2e675e7b9f01222021-12-02T12:32:03ZPathological effect of arterial ischaemia and venous congestion on rat testes10.1038/s41598-017-05880-22045-2322https://doaj.org/article/f4ef5fff811042e0ac2e675e7b9f01222017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05880-2https://doaj.org/toc/2045-2322Abstract Many studies on various organs have concluded that venous congestion (VC) causes severe organ dysfunction with elevation of oxidative stress relative to that of arterial ischaemia (AI). However, a comparison of the pathological effects of AI and VC on the testes has not been conducted. In this study, models of AI and VC and their reperfusion in rat testes, respectively, were developed and analysed. Testicular arteries or veins were interrupted for 6 h, re-perfused and kept for 4 weeks; the effects on the testes were then evaluated. Severe spermatogenic disturbances were observed at 4 weeks after reperfusion in AI but not in VC. At 6 h after blood flow interruption, oxidative stress was significantly increased and germ cells were severely damaged in AI compared with those in VC. RT-PCR analyses revealed that haem oxygenase-1, which exhibits anti-oxidative effects, and vascular endothelial growth factor-A, which exhibits vasculogenic effects, were significantly increased in VC but not in AI. Surprisingly, the results of our experiment in rat testes differed from those of experiments in previous studies performed in other organs. Oxidative stress in testes was more easily elevated by AI than it was by VC, explainable by the different experimental conditions.Shuichi HiraiNaoyuki HatayamaMunekazu NaitoKenta NagahoriShinichi KawataShogo HayashiNing QuHayato TerayamaSunao ShojiMasahiro ItohNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shuichi Hirai Naoyuki Hatayama Munekazu Naito Kenta Nagahori Shinichi Kawata Shogo Hayashi Ning Qu Hayato Terayama Sunao Shoji Masahiro Itoh Pathological effect of arterial ischaemia and venous congestion on rat testes
description	Abstract Many studies on various organs have concluded that venous congestion (VC) causes severe organ dysfunction with elevation of oxidative stress relative to that of arterial ischaemia (AI). However, a comparison of the pathological effects of AI and VC on the testes has not been conducted. In this study, models of AI and VC and their reperfusion in rat testes, respectively, were developed and analysed. Testicular arteries or veins were interrupted for 6 h, re-perfused and kept for 4 weeks; the effects on the testes were then evaluated. Severe spermatogenic disturbances were observed at 4 weeks after reperfusion in AI but not in VC. At 6 h after blood flow interruption, oxidative stress was significantly increased and germ cells were severely damaged in AI compared with those in VC. RT-PCR analyses revealed that haem oxygenase-1, which exhibits anti-oxidative effects, and vascular endothelial growth factor-A, which exhibits vasculogenic effects, were significantly increased in VC but not in AI. Surprisingly, the results of our experiment in rat testes differed from those of experiments in previous studies performed in other organs. Oxidative stress in testes was more easily elevated by AI than it was by VC, explainable by the different experimental conditions.
format	article
author	Shuichi Hirai Naoyuki Hatayama Munekazu Naito Kenta Nagahori Shinichi Kawata Shogo Hayashi Ning Qu Hayato Terayama Sunao Shoji Masahiro Itoh
author_facet	Shuichi Hirai Naoyuki Hatayama Munekazu Naito Kenta Nagahori Shinichi Kawata Shogo Hayashi Ning Qu Hayato Terayama Sunao Shoji Masahiro Itoh
author_sort	Shuichi Hirai
title	Pathological effect of arterial ischaemia and venous congestion on rat testes
title_short	Pathological effect of arterial ischaemia and venous congestion on rat testes
title_full	Pathological effect of arterial ischaemia and venous congestion on rat testes
title_fullStr	Pathological effect of arterial ischaemia and venous congestion on rat testes
title_full_unstemmed	Pathological effect of arterial ischaemia and venous congestion on rat testes
title_sort	pathological effect of arterial ischaemia and venous congestion on rat testes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f4ef5fff811042e0ac2e675e7b9f0122
work_keys_str_mv	AT shuichihirai pathologicaleffectofarterialischaemiaandvenouscongestiononrattestes AT naoyukihatayama pathologicaleffectofarterialischaemiaandvenouscongestiononrattestes AT munekazunaito pathologicaleffectofarterialischaemiaandvenouscongestiononrattestes AT kentanagahori pathologicaleffectofarterialischaemiaandvenouscongestiononrattestes AT shinichikawata pathologicaleffectofarterialischaemiaandvenouscongestiononrattestes AT shogohayashi pathologicaleffectofarterialischaemiaandvenouscongestiononrattestes AT ningqu pathologicaleffectofarterialischaemiaandvenouscongestiononrattestes AT hayatoterayama pathologicaleffectofarterialischaemiaandvenouscongestiononrattestes AT sunaoshoji pathologicaleffectofarterialischaemiaandvenouscongestiononrattestes AT masahiroitoh pathologicaleffectofarterialischaemiaandvenouscongestiononrattestes
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Pathological effect of arterial ischaemia and venous congestion on rat testes

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