Computational and experimental studies of a cell-imprinted-based integrated microfluidic device for biomedical applications

Abstract It has been proved that cell-imprinted substrates molded from template cells can be used for the re-culture of that cell while preserving its normal behavior or to differentiate the cultured stem cells into the template cell. In this study, a microfluidic device was presented to modify the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sepideh Yazdian Kashani, Mostafa Keshavarz Moraveji, Shahin Bonakdar
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f4f3e492fbb445c9a53fff6e86a5ebc9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f4f3e492fbb445c9a53fff6e86a5ebc9
record_format	dspace
spelling	oai:doaj.org-article:f4f3e492fbb445c9a53fff6e86a5ebc92021-12-02T17:52:42ZComputational and experimental studies of a cell-imprinted-based integrated microfluidic device for biomedical applications10.1038/s41598-021-91616-22045-2322https://doaj.org/article/f4f3e492fbb445c9a53fff6e86a5ebc92021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91616-2https://doaj.org/toc/2045-2322Abstract It has been proved that cell-imprinted substrates molded from template cells can be used for the re-culture of that cell while preserving its normal behavior or to differentiate the cultured stem cells into the template cell. In this study, a microfluidic device was presented to modify the previous irregular cell-imprinted substrate and increase imprinting efficiency by regular and objective cell culture. First, a cell-imprinted substrate from template cells was prepared using a microfluidic chip in a regular pattern. Another microfluidic chip with the same pattern was then aligned on the cell-imprinted substrate to create a chondrocyte-imprinted-based integrated microfluidic device. Computational fluid dynamics (CFD) simulations were used to obtain suitable conditions for injecting cells into the microfluidic chip before performing experimental evaluations. In this simulation, the effect of input flow rate, number per unit volume, and size of injected cells in two different chip sizes were examined on exerted shear stress and cell trajectories. This numerical simulation was first validated with experiments with cell lines. Finally, chondrocyte was used as template cell to evaluate the chondrogenic differentiation of adipose-derived mesenchymal stem cells (ADSCs) in the chondrocyte-imprinted-based integrated microfluidic device. ADSCs were positioned precisely on the chondrocyte patterns, and without using any chemical growth factor, their fibroblast-like morphology was modified to the spherical morphology of chondrocytes after 14 days of culture. Both immunostaining and gene expression analysis showed improvement in chondrogenic differentiation compared to traditional imprinting methods. This study demonstrated the effectiveness of cell-imprinted-based integrated microfluidic devices for biomedical applications.Sepideh Yazdian KashaniMostafa Keshavarz MoravejiShahin BonakdarNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sepideh Yazdian Kashani Mostafa Keshavarz Moraveji Shahin Bonakdar Computational and experimental studies of a cell-imprinted-based integrated microfluidic device for biomedical applications
description	Abstract It has been proved that cell-imprinted substrates molded from template cells can be used for the re-culture of that cell while preserving its normal behavior or to differentiate the cultured stem cells into the template cell. In this study, a microfluidic device was presented to modify the previous irregular cell-imprinted substrate and increase imprinting efficiency by regular and objective cell culture. First, a cell-imprinted substrate from template cells was prepared using a microfluidic chip in a regular pattern. Another microfluidic chip with the same pattern was then aligned on the cell-imprinted substrate to create a chondrocyte-imprinted-based integrated microfluidic device. Computational fluid dynamics (CFD) simulations were used to obtain suitable conditions for injecting cells into the microfluidic chip before performing experimental evaluations. In this simulation, the effect of input flow rate, number per unit volume, and size of injected cells in two different chip sizes were examined on exerted shear stress and cell trajectories. This numerical simulation was first validated with experiments with cell lines. Finally, chondrocyte was used as template cell to evaluate the chondrogenic differentiation of adipose-derived mesenchymal stem cells (ADSCs) in the chondrocyte-imprinted-based integrated microfluidic device. ADSCs were positioned precisely on the chondrocyte patterns, and without using any chemical growth factor, their fibroblast-like morphology was modified to the spherical morphology of chondrocytes after 14 days of culture. Both immunostaining and gene expression analysis showed improvement in chondrogenic differentiation compared to traditional imprinting methods. This study demonstrated the effectiveness of cell-imprinted-based integrated microfluidic devices for biomedical applications.
format	article
author	Sepideh Yazdian Kashani Mostafa Keshavarz Moraveji Shahin Bonakdar
author_facet	Sepideh Yazdian Kashani Mostafa Keshavarz Moraveji Shahin Bonakdar
author_sort	Sepideh Yazdian Kashani
title	Computational and experimental studies of a cell-imprinted-based integrated microfluidic device for biomedical applications
title_short	Computational and experimental studies of a cell-imprinted-based integrated microfluidic device for biomedical applications
title_full	Computational and experimental studies of a cell-imprinted-based integrated microfluidic device for biomedical applications
title_fullStr	Computational and experimental studies of a cell-imprinted-based integrated microfluidic device for biomedical applications
title_full_unstemmed	Computational and experimental studies of a cell-imprinted-based integrated microfluidic device for biomedical applications
title_sort	computational and experimental studies of a cell-imprinted-based integrated microfluidic device for biomedical applications
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f4f3e492fbb445c9a53fff6e86a5ebc9
work_keys_str_mv	AT sepidehyazdiankashani computationalandexperimentalstudiesofacellimprintedbasedintegratedmicrofluidicdeviceforbiomedicalapplications AT mostafakeshavarzmoraveji computationalandexperimentalstudiesofacellimprintedbasedintegratedmicrofluidicdeviceforbiomedicalapplications AT shahinbonakdar computationalandexperimentalstudiesofacellimprintedbasedintegratedmicrofluidicdeviceforbiomedicalapplications
_version_	1718379206174310400

Computational and experimental studies of a cell-imprinted-based integrated microfluidic device for biomedical applications

Ejemplares similares