A high quality draft consensus sequence of the genome of a heterozygous grapevine variety.
<h4>Background</h4>Worldwide, grapes and their derived products have a large market. The cultivated grape species Vitis vinifera has potential to become a model for fruit trees genetics. Like many plant species, it is highly heterozygous, which is an additional challenge to modern whole...
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2007
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oai:doaj.org-article:f5012c79fec148a89388d69afa681c6e2021-11-25T06:13:38ZA high quality draft consensus sequence of the genome of a heterozygous grapevine variety.1932-620310.1371/journal.pone.0001326https://doaj.org/article/f5012c79fec148a89388d69afa681c6e2007-12-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0001326https://doaj.org/toc/1932-6203<h4>Background</h4>Worldwide, grapes and their derived products have a large market. The cultivated grape species Vitis vinifera has potential to become a model for fruit trees genetics. Like many plant species, it is highly heterozygous, which is an additional challenge to modern whole genome shotgun sequencing. In this paper a high quality draft genome sequence of a cultivated clone of V. vinifera Pinot Noir is presented.<h4>Principal findings</h4>We estimate the genome size of V. vinifera to be 504.6 Mb. Genomic sequences corresponding to 477.1 Mb were assembled in 2,093 metacontigs and 435.1 Mb were anchored to the 19 linkage groups (LGs). The number of predicted genes is 29,585, of which 96.1% were assigned to LGs. This assembly of the grape genome provides candidate genes implicated in traits relevant to grapevine cultivation, such as those influencing wine quality, via secondary metabolites, and those connected with the extreme susceptibility of grape to pathogens. Single nucleotide polymorphism (SNP) distribution was consistent with a diffuse haplotype structure across the genome. Of around 2,000,000 SNPs, 1,751,176 were mapped to chromosomes and one or more of them were identified in 86.7% of anchored genes. The relative age of grape duplicated genes was estimated and this made possible to reveal a relatively recent Vitis-specific large scale duplication event concerning at least 10 chromosomes (duplication not reported before).<h4>Conclusions</h4>Sanger shotgun sequencing and highly efficient sequencing by synthesis (SBS), together with dedicated assembly programs, resolved a complex heterozygous genome. A consensus sequence of the genome and a set of mapped marker loci were generated. Homologous chromosomes of Pinot Noir differ by 11.2% of their DNA (hemizygous DNA plus chromosomal gaps). SNP markers are offered as a tool with the potential of introducing a new era in the molecular breeding of grape.Riccardo VelascoAndrey ZharkikhMichela TroggioDustin A CartwrightAlessandro CestaroDmitry PrussMassimo PindoLisa M FitzgeraldSilvia VezzulliJulia ReidGiulia MalacarneDiana IlievGiuseppina CoppolaBryan WardellDiego MichelettiTeresita MacalmaMarco FacciJeff T MitchellMichele PerazzolliGlenn EldredgePamela GattoRozan OyzerskiMarco MorettoNatalia GutinMarco StefaniniYang ChenCinzia SegalaChristine DavenportLorenzo DemattèAmy MrazJuri BattilanaKeith StormoFabrizio CostaQuanzhou TaoAzeddine Si-AmmourTim HarkinsAngie LackeyClotilde PerbostBruce TaillonAlessandra StellaVictor SolovyevJeffrey A FawcettLieven SterckKlaas VandepoeleStella M GrandoStefano ToppoClaudio MoserJerry LanchburyRobert BogdenMark SkolnickVittorio SgaramellaSatish K BhatnagarPaolo FontanaAlexander GutinYves Van de PeerFrancesco SalaminiRoberto ViolaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 2, Iss 12, p e1326 (2007) |
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DOAJ |
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| topic |
Medicine R Science Q |
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Medicine R Science Q Riccardo Velasco Andrey Zharkikh Michela Troggio Dustin A Cartwright Alessandro Cestaro Dmitry Pruss Massimo Pindo Lisa M Fitzgerald Silvia Vezzulli Julia Reid Giulia Malacarne Diana Iliev Giuseppina Coppola Bryan Wardell Diego Micheletti Teresita Macalma Marco Facci Jeff T Mitchell Michele Perazzolli Glenn Eldredge Pamela Gatto Rozan Oyzerski Marco Moretto Natalia Gutin Marco Stefanini Yang Chen Cinzia Segala Christine Davenport Lorenzo Demattè Amy Mraz Juri Battilana Keith Stormo Fabrizio Costa Quanzhou Tao Azeddine Si-Ammour Tim Harkins Angie Lackey Clotilde Perbost Bruce Taillon Alessandra Stella Victor Solovyev Jeffrey A Fawcett Lieven Sterck Klaas Vandepoele Stella M Grando Stefano Toppo Claudio Moser Jerry Lanchbury Robert Bogden Mark Skolnick Vittorio Sgaramella Satish K Bhatnagar Paolo Fontana Alexander Gutin Yves Van de Peer Francesco Salamini Roberto Viola A high quality draft consensus sequence of the genome of a heterozygous grapevine variety. |
| description |
<h4>Background</h4>Worldwide, grapes and their derived products have a large market. The cultivated grape species Vitis vinifera has potential to become a model for fruit trees genetics. Like many plant species, it is highly heterozygous, which is an additional challenge to modern whole genome shotgun sequencing. In this paper a high quality draft genome sequence of a cultivated clone of V. vinifera Pinot Noir is presented.<h4>Principal findings</h4>We estimate the genome size of V. vinifera to be 504.6 Mb. Genomic sequences corresponding to 477.1 Mb were assembled in 2,093 metacontigs and 435.1 Mb were anchored to the 19 linkage groups (LGs). The number of predicted genes is 29,585, of which 96.1% were assigned to LGs. This assembly of the grape genome provides candidate genes implicated in traits relevant to grapevine cultivation, such as those influencing wine quality, via secondary metabolites, and those connected with the extreme susceptibility of grape to pathogens. Single nucleotide polymorphism (SNP) distribution was consistent with a diffuse haplotype structure across the genome. Of around 2,000,000 SNPs, 1,751,176 were mapped to chromosomes and one or more of them were identified in 86.7% of anchored genes. The relative age of grape duplicated genes was estimated and this made possible to reveal a relatively recent Vitis-specific large scale duplication event concerning at least 10 chromosomes (duplication not reported before).<h4>Conclusions</h4>Sanger shotgun sequencing and highly efficient sequencing by synthesis (SBS), together with dedicated assembly programs, resolved a complex heterozygous genome. A consensus sequence of the genome and a set of mapped marker loci were generated. Homologous chromosomes of Pinot Noir differ by 11.2% of their DNA (hemizygous DNA plus chromosomal gaps). SNP markers are offered as a tool with the potential of introducing a new era in the molecular breeding of grape. |
| format |
article |
| author |
Riccardo Velasco Andrey Zharkikh Michela Troggio Dustin A Cartwright Alessandro Cestaro Dmitry Pruss Massimo Pindo Lisa M Fitzgerald Silvia Vezzulli Julia Reid Giulia Malacarne Diana Iliev Giuseppina Coppola Bryan Wardell Diego Micheletti Teresita Macalma Marco Facci Jeff T Mitchell Michele Perazzolli Glenn Eldredge Pamela Gatto Rozan Oyzerski Marco Moretto Natalia Gutin Marco Stefanini Yang Chen Cinzia Segala Christine Davenport Lorenzo Demattè Amy Mraz Juri Battilana Keith Stormo Fabrizio Costa Quanzhou Tao Azeddine Si-Ammour Tim Harkins Angie Lackey Clotilde Perbost Bruce Taillon Alessandra Stella Victor Solovyev Jeffrey A Fawcett Lieven Sterck Klaas Vandepoele Stella M Grando Stefano Toppo Claudio Moser Jerry Lanchbury Robert Bogden Mark Skolnick Vittorio Sgaramella Satish K Bhatnagar Paolo Fontana Alexander Gutin Yves Van de Peer Francesco Salamini Roberto Viola |
| author_facet |
Riccardo Velasco Andrey Zharkikh Michela Troggio Dustin A Cartwright Alessandro Cestaro Dmitry Pruss Massimo Pindo Lisa M Fitzgerald Silvia Vezzulli Julia Reid Giulia Malacarne Diana Iliev Giuseppina Coppola Bryan Wardell Diego Micheletti Teresita Macalma Marco Facci Jeff T Mitchell Michele Perazzolli Glenn Eldredge Pamela Gatto Rozan Oyzerski Marco Moretto Natalia Gutin Marco Stefanini Yang Chen Cinzia Segala Christine Davenport Lorenzo Demattè Amy Mraz Juri Battilana Keith Stormo Fabrizio Costa Quanzhou Tao Azeddine Si-Ammour Tim Harkins Angie Lackey Clotilde Perbost Bruce Taillon Alessandra Stella Victor Solovyev Jeffrey A Fawcett Lieven Sterck Klaas Vandepoele Stella M Grando Stefano Toppo Claudio Moser Jerry Lanchbury Robert Bogden Mark Skolnick Vittorio Sgaramella Satish K Bhatnagar Paolo Fontana Alexander Gutin Yves Van de Peer Francesco Salamini Roberto Viola |
| author_sort |
Riccardo Velasco |
| title |
A high quality draft consensus sequence of the genome of a heterozygous grapevine variety. |
| title_short |
A high quality draft consensus sequence of the genome of a heterozygous grapevine variety. |
| title_full |
A high quality draft consensus sequence of the genome of a heterozygous grapevine variety. |
| title_fullStr |
A high quality draft consensus sequence of the genome of a heterozygous grapevine variety. |
| title_full_unstemmed |
A high quality draft consensus sequence of the genome of a heterozygous grapevine variety. |
| title_sort |
high quality draft consensus sequence of the genome of a heterozygous grapevine variety. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2007 |
| url |
https://doaj.org/article/f5012c79fec148a89388d69afa681c6e |
| work_keys_str_mv |
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