EFFECTS OF HELPER AND REGULATORY CELLS UPON PHENOTYPIC COMPOSITION OF BLOOD B LYMPHOCYTES AND THYROID GLAND IN GRAVES’ DISEASE

The aim of this work was a comparative study of the helper- (Th cells) and regulatory T cells (Treg) effects upon the phenotypic composition of B lymphocytes in blood and thyroid tissue in Graves’ disease (GD). 43 women with GD were examined. The diagnosis of GD was based on clinical and laboratory...

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Autores principales:	A. A. Savchenko, M. A. Dudina, A. G. Borisov, S. A. Dogadin, I. V. Kudryavtsev, A. V. Moshev, V. А. Mankovskiy
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2018
Materias:	t regulatory cells t helpers b lymphocytes subpopulation blood thyroid tissue graves’ disease Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/f5057ff2eb434bd0a79dd378b845e57e
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spelling	oai:doaj.org-article:f5057ff2eb434bd0a79dd378b845e57e2021-11-18T08:03:47ZEFFECTS OF HELPER AND REGULATORY CELLS UPON PHENOTYPIC COMPOSITION OF BLOOD B LYMPHOCYTES AND THYROID GLAND IN GRAVES’ DISEASE1563-06252313-741X10.15789/1563-0625-2018-3-431-438https://doaj.org/article/f5057ff2eb434bd0a79dd378b845e57e2018-06-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1534https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XThe aim of this work was a comparative study of the helper- (Th cells) and regulatory T cells (Treg) effects upon the phenotypic composition of B lymphocytes in blood and thyroid tissue in Graves’ disease (GD). 43 women with GD were examined. The diagnosis of GD was based on clinical and laboratory signs of the disease: complaints, clinical picture of the thyrotoxicosis with objective examination, characteristic sonographic changes in thyroid gland, as well as elevated titers of antibodies to thyroid-stimulating hormone receptor in blood serum, and corresponding changes in thyroid status. 67 practically healthy women were examined as a control. The studies of Th cells, Treg and B lymphocytes phenotypes in blood and thyroid tissue were carried out by flow cytometry using direct immunofluorescence, respectively, in whole peripheral blood and lymphocytes isolated from thyroid tissue. The relative amounts of Tregs in thyroid gland from the patients with GD corresponds to their level in the blood. We did not find any changes in the content of blood T helpers expressing vs. non-expressing CD25 receptors, as compared to the control values. In patients with GD, an increased B1 cells content was revealed in peripheral blood. The percentage of this B cell subpopulation in thyroid tissue is reduced when compared to the levels found in blood, but with increased memory B cells contents. The number of activated B lymphocytes (by CD23 marker) in blood of patients with GD is reduced when compared to control values. It was found that, in thyroid tissue, there is an even more pronounced decrease in the relative amount of activated B cells compared to the levels detected in blood from these patients. By means of correlation analysis, it was found that increase in activated B lymphocytes in blood from controls is accompanied by a co-directional reaction from Treg (the usual immunoregulatory process). In Graves’ disease, such a relationship was not found. The amounts of Treg and activated T helper cells in blood of the patients did positively correlate with common B lymphocytes, B2 cells and na ve B lymphocytes. Meanwhile, Treg’s in thyroid tissue, were completely excluded from the system of interactions with activated B lymphocytes. It is assumed that a decrease in Treg’s content in peripheral blood, along with altered functional activity is observed in patients with GD.A. A. SavchenkoM. A. DudinaA. G. BorisovS. A. DogadinI. V. KudryavtsevA. V. MoshevV. А. MankovskiySPb RAACIarticlet regulatory cellst helpersb lymphocytessubpopulationbloodthyroid tissuegraves’ diseaseImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 20, Iss 3, Pp 431-438 (2018)
institution	DOAJ
collection	DOAJ
language	RU
topic	t regulatory cells t helpers b lymphocytes subpopulation blood thyroid tissue graves’ disease Immunologic diseases. Allergy RC581-607
spellingShingle	t regulatory cells t helpers b lymphocytes subpopulation blood thyroid tissue graves’ disease Immunologic diseases. Allergy RC581-607 A. A. Savchenko M. A. Dudina A. G. Borisov S. A. Dogadin I. V. Kudryavtsev A. V. Moshev V. А. Mankovskiy EFFECTS OF HELPER AND REGULATORY CELLS UPON PHENOTYPIC COMPOSITION OF BLOOD B LYMPHOCYTES AND THYROID GLAND IN GRAVES’ DISEASE
description	The aim of this work was a comparative study of the helper- (Th cells) and regulatory T cells (Treg) effects upon the phenotypic composition of B lymphocytes in blood and thyroid tissue in Graves’ disease (GD). 43 women with GD were examined. The diagnosis of GD was based on clinical and laboratory signs of the disease: complaints, clinical picture of the thyrotoxicosis with objective examination, characteristic sonographic changes in thyroid gland, as well as elevated titers of antibodies to thyroid-stimulating hormone receptor in blood serum, and corresponding changes in thyroid status. 67 practically healthy women were examined as a control. The studies of Th cells, Treg and B lymphocytes phenotypes in blood and thyroid tissue were carried out by flow cytometry using direct immunofluorescence, respectively, in whole peripheral blood and lymphocytes isolated from thyroid tissue. The relative amounts of Tregs in thyroid gland from the patients with GD corresponds to their level in the blood. We did not find any changes in the content of blood T helpers expressing vs. non-expressing CD25 receptors, as compared to the control values. In patients with GD, an increased B1 cells content was revealed in peripheral blood. The percentage of this B cell subpopulation in thyroid tissue is reduced when compared to the levels found in blood, but with increased memory B cells contents. The number of activated B lymphocytes (by CD23 marker) in blood of patients with GD is reduced when compared to control values. It was found that, in thyroid tissue, there is an even more pronounced decrease in the relative amount of activated B cells compared to the levels detected in blood from these patients. By means of correlation analysis, it was found that increase in activated B lymphocytes in blood from controls is accompanied by a co-directional reaction from Treg (the usual immunoregulatory process). In Graves’ disease, such a relationship was not found. The amounts of Treg and activated T helper cells in blood of the patients did positively correlate with common B lymphocytes, B2 cells and na ve B lymphocytes. Meanwhile, Treg’s in thyroid tissue, were completely excluded from the system of interactions with activated B lymphocytes. It is assumed that a decrease in Treg’s content in peripheral blood, along with altered functional activity is observed in patients with GD.
format	article
author	A. A. Savchenko M. A. Dudina A. G. Borisov S. A. Dogadin I. V. Kudryavtsev A. V. Moshev V. А. Mankovskiy
author_facet	A. A. Savchenko M. A. Dudina A. G. Borisov S. A. Dogadin I. V. Kudryavtsev A. V. Moshev V. А. Mankovskiy
author_sort	A. A. Savchenko
title	EFFECTS OF HELPER AND REGULATORY CELLS UPON PHENOTYPIC COMPOSITION OF BLOOD B LYMPHOCYTES AND THYROID GLAND IN GRAVES’ DISEASE
title_short	EFFECTS OF HELPER AND REGULATORY CELLS UPON PHENOTYPIC COMPOSITION OF BLOOD B LYMPHOCYTES AND THYROID GLAND IN GRAVES’ DISEASE
title_full	EFFECTS OF HELPER AND REGULATORY CELLS UPON PHENOTYPIC COMPOSITION OF BLOOD B LYMPHOCYTES AND THYROID GLAND IN GRAVES’ DISEASE
title_fullStr	EFFECTS OF HELPER AND REGULATORY CELLS UPON PHENOTYPIC COMPOSITION OF BLOOD B LYMPHOCYTES AND THYROID GLAND IN GRAVES’ DISEASE
title_full_unstemmed	EFFECTS OF HELPER AND REGULATORY CELLS UPON PHENOTYPIC COMPOSITION OF BLOOD B LYMPHOCYTES AND THYROID GLAND IN GRAVES’ DISEASE
title_sort	effects of helper and regulatory cells upon phenotypic composition of blood b lymphocytes and thyroid gland in graves’ disease
publisher	SPb RAACI
publishDate	2018
url	https://doaj.org/article/f5057ff2eb434bd0a79dd378b845e57e
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EFFECTS OF HELPER AND REGULATORY CELLS UPON PHENOTYPIC COMPOSITION OF BLOOD B LYMPHOCYTES AND THYROID GLAND IN GRAVES’ DISEASE

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