The nuclear import of the transcription factor MyoD is reduced in mesenchymal stem cells grown in a 3D micro-engineered niche

Abstract Smart biomaterials are increasingly being used to control stem cell fate in vitro by the recapitulation of the native niche microenvironment. By integrating experimental measurements with numerical models, we show that in mesenchymal stem cells grown inside a 3D synthetic niche both nuclear...

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Autores principales:	Emanuela Jacchetti, Ramin Nasehi, Lucia Boeri, Valentina Parodi, Alessandro Negro, Diego Albani, Roberto Osellame, Giulio Cerullo, Jose Felix Rodriguez Matas, Manuela Teresa Raimondi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f506a71168f74e929134b61056b23a77
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spelling	oai:doaj.org-article:f506a71168f74e929134b61056b23a772021-12-02T10:44:14ZThe nuclear import of the transcription factor MyoD is reduced in mesenchymal stem cells grown in a 3D micro-engineered niche10.1038/s41598-021-81920-22045-2322https://doaj.org/article/f506a71168f74e929134b61056b23a772021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81920-2https://doaj.org/toc/2045-2322Abstract Smart biomaterials are increasingly being used to control stem cell fate in vitro by the recapitulation of the native niche microenvironment. By integrating experimental measurements with numerical models, we show that in mesenchymal stem cells grown inside a 3D synthetic niche both nuclear transport of a myogenic factor and the passive nuclear diffusion of a smaller inert protein are reduced. Our results also suggest that cell morphology modulates nuclear proteins import through a partition of the nuclear envelope surface, which is a thin but extremely permeable annular portion in cells cultured on 2D substrates. Therefore, our results support the hypothesis that in stem cell differentiation, the nuclear import of gene-regulating transcription factors is controlled by a strain-dependent nuclear envelope permeability, probably related to the reorganization of stretch-activated nuclear pore complexes.Emanuela JacchettiRamin NasehiLucia BoeriValentina ParodiAlessandro NegroDiego AlbaniRoberto OsellameGiulio CerulloJose Felix Rodriguez MatasManuela Teresa RaimondiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-19 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Emanuela Jacchetti Ramin Nasehi Lucia Boeri Valentina Parodi Alessandro Negro Diego Albani Roberto Osellame Giulio Cerullo Jose Felix Rodriguez Matas Manuela Teresa Raimondi The nuclear import of the transcription factor MyoD is reduced in mesenchymal stem cells grown in a 3D micro-engineered niche
description	Abstract Smart biomaterials are increasingly being used to control stem cell fate in vitro by the recapitulation of the native niche microenvironment. By integrating experimental measurements with numerical models, we show that in mesenchymal stem cells grown inside a 3D synthetic niche both nuclear transport of a myogenic factor and the passive nuclear diffusion of a smaller inert protein are reduced. Our results also suggest that cell morphology modulates nuclear proteins import through a partition of the nuclear envelope surface, which is a thin but extremely permeable annular portion in cells cultured on 2D substrates. Therefore, our results support the hypothesis that in stem cell differentiation, the nuclear import of gene-regulating transcription factors is controlled by a strain-dependent nuclear envelope permeability, probably related to the reorganization of stretch-activated nuclear pore complexes.
format	article
author	Emanuela Jacchetti Ramin Nasehi Lucia Boeri Valentina Parodi Alessandro Negro Diego Albani Roberto Osellame Giulio Cerullo Jose Felix Rodriguez Matas Manuela Teresa Raimondi
author_facet	Emanuela Jacchetti Ramin Nasehi Lucia Boeri Valentina Parodi Alessandro Negro Diego Albani Roberto Osellame Giulio Cerullo Jose Felix Rodriguez Matas Manuela Teresa Raimondi
author_sort	Emanuela Jacchetti
title	The nuclear import of the transcription factor MyoD is reduced in mesenchymal stem cells grown in a 3D micro-engineered niche
title_short	The nuclear import of the transcription factor MyoD is reduced in mesenchymal stem cells grown in a 3D micro-engineered niche
title_full	The nuclear import of the transcription factor MyoD is reduced in mesenchymal stem cells grown in a 3D micro-engineered niche
title_fullStr	The nuclear import of the transcription factor MyoD is reduced in mesenchymal stem cells grown in a 3D micro-engineered niche
title_full_unstemmed	The nuclear import of the transcription factor MyoD is reduced in mesenchymal stem cells grown in a 3D micro-engineered niche
title_sort	nuclear import of the transcription factor myod is reduced in mesenchymal stem cells grown in a 3d micro-engineered niche
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f506a71168f74e929134b61056b23a77
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The nuclear import of the transcription factor MyoD is reduced in mesenchymal stem cells grown in a 3D micro-engineered niche

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