Urea transporter UT-B deletion induces DNA damage and apoptosis in mouse bladder urothelium.

<h4>Background</h4>Previous studies found that urea transporter UT-B is abundantly expressed in bladder urothelium. However, the dynamic role of UT-B in bladder urothelial cells remains unclear. The objective of this study is to evaluate the physiological roles of UT-B in bladder urothel...

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Autores principales:	Zixun Dong, Jianhua Ran, Hong Zhou, Jihui Chen, Tianluo Lei, Weiling Wang, Yi Sun, Guiting Lin, Lise Bankir, Baoxue Yang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f508f0d209134268b6778adc24998ae9
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spelling	oai:doaj.org-article:f508f0d209134268b6778adc24998ae92021-11-18T08:50:13ZUrea transporter UT-B deletion induces DNA damage and apoptosis in mouse bladder urothelium.1932-620310.1371/journal.pone.0076952https://doaj.org/article/f508f0d209134268b6778adc24998ae92013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24204711/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Previous studies found that urea transporter UT-B is abundantly expressed in bladder urothelium. However, the dynamic role of UT-B in bladder urothelial cells remains unclear. The objective of this study is to evaluate the physiological roles of UT-B in bladder urothelium using UT-B knockout mouse model and T24 cell line.<h4>Methodology/principal findings</h4>Urea and NO measurement, mRNA expression micro-array analysis, light and transmission electron microscopy, apoptosis assays, DNA damage and repair determination, and intracellular signaling examination were performed in UT-B null bladders vs wild-type bladders and in vitro T24 epithelial cells. UT-B was highly expressed in mouse bladder urothelium. The genes, Dcaf11, MCM2-4, Uch-L1, Bnip3 and 45 S pre rRNA, related to DNA damage and apoptosis were significantly regulated in UT-B null urothelium. DNA damage and apoptosis highly occurred in UT-B null urothelium. Urea and NO levels were significantly higher in UT-B null urothelium than that in wild-type, which may affect L-arginine metabolism and the intracellular signals related to DNA damage and apoptosis. These findings were consistent with the in vitro study in T24 cells that, after urea loading, exhibited cell cycle delay and apoptosis.<h4>Conclusions/significance</h4>UT-B may play an important role in protecting bladder urothelium by balancing intracellular urea concentration. Disruption of UT-B function induces DNA damage and apoptosis in bladder, which can result in bladder disorders.Zixun DongJianhua RanHong ZhouJihui ChenTianluo LeiWeiling WangYi SunGuiting LinLise BankirBaoxue YangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 10, p e76952 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zixun Dong Jianhua Ran Hong Zhou Jihui Chen Tianluo Lei Weiling Wang Yi Sun Guiting Lin Lise Bankir Baoxue Yang Urea transporter UT-B deletion induces DNA damage and apoptosis in mouse bladder urothelium.
description	<h4>Background</h4>Previous studies found that urea transporter UT-B is abundantly expressed in bladder urothelium. However, the dynamic role of UT-B in bladder urothelial cells remains unclear. The objective of this study is to evaluate the physiological roles of UT-B in bladder urothelium using UT-B knockout mouse model and T24 cell line.<h4>Methodology/principal findings</h4>Urea and NO measurement, mRNA expression micro-array analysis, light and transmission electron microscopy, apoptosis assays, DNA damage and repair determination, and intracellular signaling examination were performed in UT-B null bladders vs wild-type bladders and in vitro T24 epithelial cells. UT-B was highly expressed in mouse bladder urothelium. The genes, Dcaf11, MCM2-4, Uch-L1, Bnip3 and 45 S pre rRNA, related to DNA damage and apoptosis were significantly regulated in UT-B null urothelium. DNA damage and apoptosis highly occurred in UT-B null urothelium. Urea and NO levels were significantly higher in UT-B null urothelium than that in wild-type, which may affect L-arginine metabolism and the intracellular signals related to DNA damage and apoptosis. These findings were consistent with the in vitro study in T24 cells that, after urea loading, exhibited cell cycle delay and apoptosis.<h4>Conclusions/significance</h4>UT-B may play an important role in protecting bladder urothelium by balancing intracellular urea concentration. Disruption of UT-B function induces DNA damage and apoptosis in bladder, which can result in bladder disorders.
format	article
author	Zixun Dong Jianhua Ran Hong Zhou Jihui Chen Tianluo Lei Weiling Wang Yi Sun Guiting Lin Lise Bankir Baoxue Yang
author_facet	Zixun Dong Jianhua Ran Hong Zhou Jihui Chen Tianluo Lei Weiling Wang Yi Sun Guiting Lin Lise Bankir Baoxue Yang
author_sort	Zixun Dong
title	Urea transporter UT-B deletion induces DNA damage and apoptosis in mouse bladder urothelium.
title_short	Urea transporter UT-B deletion induces DNA damage and apoptosis in mouse bladder urothelium.
title_full	Urea transporter UT-B deletion induces DNA damage and apoptosis in mouse bladder urothelium.
title_fullStr	Urea transporter UT-B deletion induces DNA damage and apoptosis in mouse bladder urothelium.
title_full_unstemmed	Urea transporter UT-B deletion induces DNA damage and apoptosis in mouse bladder urothelium.
title_sort	urea transporter ut-b deletion induces dna damage and apoptosis in mouse bladder urothelium.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/f508f0d209134268b6778adc24998ae9
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_version_	1718421299399753728

Urea transporter UT-B deletion induces DNA damage and apoptosis in mouse bladder urothelium.

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