Surface chemistry for cytosolic gene delivery and photothermal transgene expression by gold nanorods

Abstract Light-inducible gene regulation has great potential for remote and noninvasive control of the fate and function of target cells. One method to achieve such control is delivery of heat shock protein (HSP) promoter-driven protein expression vectors and photothermal heaters into the cells, fol...

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Autores principales:	Hirotaka Nakatsuji, Kelly Kawabata Galbraith, Junko Kurisu, Hiroshi Imahori, Tatsuya Murakami, Mineko Kengaku
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f50c028d29114215b588e4cd96a8f5a8
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spelling	oai:doaj.org-article:f50c028d29114215b588e4cd96a8f5a82021-12-02T16:08:20ZSurface chemistry for cytosolic gene delivery and photothermal transgene expression by gold nanorods10.1038/s41598-017-04912-12045-2322https://doaj.org/article/f50c028d29114215b588e4cd96a8f5a82017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04912-1https://doaj.org/toc/2045-2322Abstract Light-inducible gene regulation has great potential for remote and noninvasive control of the fate and function of target cells. One method to achieve such control is delivery of heat shock protein (HSP) promoter-driven protein expression vectors and photothermal heaters into the cells, followed by activation by illumination. In this study, we show that gold nanorods (AuNRs) functionalized with two conventional lipids, oleate and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), are capable of efficient transfection and quick photoactivation of the HSP promoter. Use of our AuNRs (DOTAP-AuNRs) was comparable to Lipofectamine 2000 in terms of transfection efficiency, while lower in cytotoxicity. Subsequent near-infrared laser (NIR) illumination of the cells transfected by DOTAP-AuNRs for 10 s induced time- and site-specific transgene expression without significant phototoxicity, to a degree similar to that of heating the entire culture dish for 30 min. Our mechanistic studies suggest that efficient transfection and quick photoactivation of the HSP promoter (HSP70b’) are due to the promoted endosomal escape of DOTAP-AuNRs. We propose a novel protocol for NIR-inducible, site-directed gene expression using an unprecedented complex of the three conventional components capable of both transfection and photothermal heating.Hirotaka NakatsujiKelly Kawabata GalbraithJunko KurisuHiroshi ImahoriTatsuya MurakamiMineko KengakuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hirotaka Nakatsuji Kelly Kawabata Galbraith Junko Kurisu Hiroshi Imahori Tatsuya Murakami Mineko Kengaku Surface chemistry for cytosolic gene delivery and photothermal transgene expression by gold nanorods
description	Abstract Light-inducible gene regulation has great potential for remote and noninvasive control of the fate and function of target cells. One method to achieve such control is delivery of heat shock protein (HSP) promoter-driven protein expression vectors and photothermal heaters into the cells, followed by activation by illumination. In this study, we show that gold nanorods (AuNRs) functionalized with two conventional lipids, oleate and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), are capable of efficient transfection and quick photoactivation of the HSP promoter. Use of our AuNRs (DOTAP-AuNRs) was comparable to Lipofectamine 2000 in terms of transfection efficiency, while lower in cytotoxicity. Subsequent near-infrared laser (NIR) illumination of the cells transfected by DOTAP-AuNRs for 10 s induced time- and site-specific transgene expression without significant phototoxicity, to a degree similar to that of heating the entire culture dish for 30 min. Our mechanistic studies suggest that efficient transfection and quick photoactivation of the HSP promoter (HSP70b’) are due to the promoted endosomal escape of DOTAP-AuNRs. We propose a novel protocol for NIR-inducible, site-directed gene expression using an unprecedented complex of the three conventional components capable of both transfection and photothermal heating.
format	article
author	Hirotaka Nakatsuji Kelly Kawabata Galbraith Junko Kurisu Hiroshi Imahori Tatsuya Murakami Mineko Kengaku
author_facet	Hirotaka Nakatsuji Kelly Kawabata Galbraith Junko Kurisu Hiroshi Imahori Tatsuya Murakami Mineko Kengaku
author_sort	Hirotaka Nakatsuji
title	Surface chemistry for cytosolic gene delivery and photothermal transgene expression by gold nanorods
title_short	Surface chemistry for cytosolic gene delivery and photothermal transgene expression by gold nanorods
title_full	Surface chemistry for cytosolic gene delivery and photothermal transgene expression by gold nanorods
title_fullStr	Surface chemistry for cytosolic gene delivery and photothermal transgene expression by gold nanorods
title_full_unstemmed	Surface chemistry for cytosolic gene delivery and photothermal transgene expression by gold nanorods
title_sort	surface chemistry for cytosolic gene delivery and photothermal transgene expression by gold nanorods
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f50c028d29114215b588e4cd96a8f5a8
work_keys_str_mv	AT hirotakanakatsuji surfacechemistryforcytosolicgenedeliveryandphotothermaltransgeneexpressionbygoldnanorods AT kellykawabatagalbraith surfacechemistryforcytosolicgenedeliveryandphotothermaltransgeneexpressionbygoldnanorods AT junkokurisu surfacechemistryforcytosolicgenedeliveryandphotothermaltransgeneexpressionbygoldnanorods AT hiroshiimahori surfacechemistryforcytosolicgenedeliveryandphotothermaltransgeneexpressionbygoldnanorods AT tatsuyamurakami surfacechemistryforcytosolicgenedeliveryandphotothermaltransgeneexpressionbygoldnanorods AT minekokengaku surfacechemistryforcytosolicgenedeliveryandphotothermaltransgeneexpressionbygoldnanorods
_version_	1718384570917715968

Surface chemistry for cytosolic gene delivery and photothermal transgene expression by gold nanorods

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