In Vitro Evaluation of DSPE-PEG (5000) Amine SWCNT Toxicity and Efficacy as a Novel Nanovector Candidate in Photothermal Therapy by Response Surface Methodology (RSM)

Nowadays, finding a novel, effective, biocompatible, and minimally invasive cancer treatment is of great importance. One of the most promising research fields is the development of biocompatible photothermal nanocarriers. PTT (photothermal therapy) with an NIR (near-infrared) wavelength range (700–2...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Naghmeh Hadidi, Niloufar Shahbahrami Moghadam, Gholamreza Pazuki, Parviz Parvin, Fatemeh Shahi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	SWCNT toxicity photothermal therapy DSPE-PEG (5000) amine response surface methodology (RSM) SKOV3 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f512900e20984050a7d6b6601b104cf7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f512900e20984050a7d6b6601b104cf7
record_format	dspace
spelling	oai:doaj.org-article:f512900e20984050a7d6b6601b104cf72021-11-25T17:08:23ZIn Vitro Evaluation of DSPE-PEG (5000) Amine SWCNT Toxicity and Efficacy as a Novel Nanovector Candidate in Photothermal Therapy by Response Surface Methodology (RSM)10.3390/cells101128742073-4409https://doaj.org/article/f512900e20984050a7d6b6601b104cf72021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/2874https://doaj.org/toc/2073-4409Nowadays, finding a novel, effective, biocompatible, and minimally invasive cancer treatment is of great importance. One of the most promising research fields is the development of biocompatible photothermal nanocarriers. PTT (photothermal therapy) with an NIR (near-infrared) wavelength range (700–2000 nm) would cause cell death by increasing intercellular and intracellular temperature. PTT could also be helpful to overcome drug resistance during cancer treatments. In this study, an amine derivative of phospholipid poly ethylene glycol (DSPE-PEG (5000) amine) was conjugated with SWCNTs (single-walled carbon nanotubes) to reduce their intrinsic toxicity. Toxicity studies were performed on lung, liver, and ovarian cancer cell lines that were reported to show some degree of drug resistance to cisplatin. Toxicity results suggested that DSPE-PEG (5000) amine SWCNTs might be biocompatible photothermal nanocarriers in PTT. Therefore, our next step was to investigate the effect of DSPE-PEG (5000) amine SWCNT concentration, cell treatment time, and laser fluence on the apoptosis/necrosis of SKOV3 cells post-NIR exposure by RSM and experimental design software. It was concluded that photothermal efficacy and total apoptosis would be dose-dependent in terms of DSPE-PEG (5000) amine SWCNT concentration and fluence. Optimal solutions which showed the highest apoptosis and lowest necrosis were then achieved.Naghmeh HadidiNiloufar Shahbahrami MoghadamGholamreza PazukiParviz ParvinFatemeh ShahiMDPI AGarticleSWCNTtoxicityphotothermal therapyDSPE-PEG (5000) amineresponse surface methodology (RSM)SKOV3Biology (General)QH301-705.5ENCells, Vol 10, Iss 2874, p 2874 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	SWCNT toxicity photothermal therapy DSPE-PEG (5000) amine response surface methodology (RSM) SKOV3 Biology (General) QH301-705.5
spellingShingle	SWCNT toxicity photothermal therapy DSPE-PEG (5000) amine response surface methodology (RSM) SKOV3 Biology (General) QH301-705.5 Naghmeh Hadidi Niloufar Shahbahrami Moghadam Gholamreza Pazuki Parviz Parvin Fatemeh Shahi In Vitro Evaluation of DSPE-PEG (5000) Amine SWCNT Toxicity and Efficacy as a Novel Nanovector Candidate in Photothermal Therapy by Response Surface Methodology (RSM)
description	Nowadays, finding a novel, effective, biocompatible, and minimally invasive cancer treatment is of great importance. One of the most promising research fields is the development of biocompatible photothermal nanocarriers. PTT (photothermal therapy) with an NIR (near-infrared) wavelength range (700–2000 nm) would cause cell death by increasing intercellular and intracellular temperature. PTT could also be helpful to overcome drug resistance during cancer treatments. In this study, an amine derivative of phospholipid poly ethylene glycol (DSPE-PEG (5000) amine) was conjugated with SWCNTs (single-walled carbon nanotubes) to reduce their intrinsic toxicity. Toxicity studies were performed on lung, liver, and ovarian cancer cell lines that were reported to show some degree of drug resistance to cisplatin. Toxicity results suggested that DSPE-PEG (5000) amine SWCNTs might be biocompatible photothermal nanocarriers in PTT. Therefore, our next step was to investigate the effect of DSPE-PEG (5000) amine SWCNT concentration, cell treatment time, and laser fluence on the apoptosis/necrosis of SKOV3 cells post-NIR exposure by RSM and experimental design software. It was concluded that photothermal efficacy and total apoptosis would be dose-dependent in terms of DSPE-PEG (5000) amine SWCNT concentration and fluence. Optimal solutions which showed the highest apoptosis and lowest necrosis were then achieved.
format	article
author	Naghmeh Hadidi Niloufar Shahbahrami Moghadam Gholamreza Pazuki Parviz Parvin Fatemeh Shahi
author_facet	Naghmeh Hadidi Niloufar Shahbahrami Moghadam Gholamreza Pazuki Parviz Parvin Fatemeh Shahi
author_sort	Naghmeh Hadidi
title	In Vitro Evaluation of DSPE-PEG (5000) Amine SWCNT Toxicity and Efficacy as a Novel Nanovector Candidate in Photothermal Therapy by Response Surface Methodology (RSM)
title_short	In Vitro Evaluation of DSPE-PEG (5000) Amine SWCNT Toxicity and Efficacy as a Novel Nanovector Candidate in Photothermal Therapy by Response Surface Methodology (RSM)
title_full	In Vitro Evaluation of DSPE-PEG (5000) Amine SWCNT Toxicity and Efficacy as a Novel Nanovector Candidate in Photothermal Therapy by Response Surface Methodology (RSM)
title_fullStr	In Vitro Evaluation of DSPE-PEG (5000) Amine SWCNT Toxicity and Efficacy as a Novel Nanovector Candidate in Photothermal Therapy by Response Surface Methodology (RSM)
title_full_unstemmed	In Vitro Evaluation of DSPE-PEG (5000) Amine SWCNT Toxicity and Efficacy as a Novel Nanovector Candidate in Photothermal Therapy by Response Surface Methodology (RSM)
title_sort	in vitro evaluation of dspe-peg (5000) amine swcnt toxicity and efficacy as a novel nanovector candidate in photothermal therapy by response surface methodology (rsm)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f512900e20984050a7d6b6601b104cf7
work_keys_str_mv	AT naghmehhadidi invitroevaluationofdspepeg5000amineswcnttoxicityandefficacyasanovelnanovectorcandidateinphotothermaltherapybyresponsesurfacemethodologyrsm AT niloufarshahbahramimoghadam invitroevaluationofdspepeg5000amineswcnttoxicityandefficacyasanovelnanovectorcandidateinphotothermaltherapybyresponsesurfacemethodologyrsm AT gholamrezapazuki invitroevaluationofdspepeg5000amineswcnttoxicityandefficacyasanovelnanovectorcandidateinphotothermaltherapybyresponsesurfacemethodologyrsm AT parvizparvin invitroevaluationofdspepeg5000amineswcnttoxicityandefficacyasanovelnanovectorcandidateinphotothermaltherapybyresponsesurfacemethodologyrsm AT fatemehshahi invitroevaluationofdspepeg5000amineswcnttoxicityandefficacyasanovelnanovectorcandidateinphotothermaltherapybyresponsesurfacemethodologyrsm
_version_	1718412750698315776

In Vitro Evaluation of DSPE-PEG (5000) Amine SWCNT Toxicity and Efficacy as a Novel Nanovector Candidate in Photothermal Therapy by Response Surface Methodology (RSM)

Ejemplares similares