Respiratory Syncytial Virus Infection Induces Expression of Inducible Nitric Oxide Synthase, CD3, and CD8 in Naturally Occurring Pneumonia in Lambs

Respiratory syncytial virus (RSV) is an RNA virus that belongs to the Pneumovirus genus of the Paramyxoviridae family. The aim of this study was to evaluate the expressions of inducible nitric oxide synthetase (iNOS), CD3 (pan T cells), and CD8 (cytotoxic T cells) in lamb lungs naturally infected wi...

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Autores principales:	Yaman Turan, Aydemir Ceyhun
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2021
Materias:	cd3 cd8 immunohistochemistry inos lamb rsv Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/f5225c94a3cd44e2a8d9410f2612478d
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spelling	oai:doaj.org-article:f5225c94a3cd44e2a8d9410f2612478d2021-11-17T21:27:54ZRespiratory Syncytial Virus Infection Induces Expression of Inducible Nitric Oxide Synthase, CD3, and CD8 in Naturally Occurring Pneumonia in Lambs1820-744810.2478/acve-2021-0015https://doaj.org/article/f5225c94a3cd44e2a8d9410f2612478d2021-06-01T00:00:00Zhttps://doi.org/10.2478/acve-2021-0015https://doaj.org/toc/1820-7448Respiratory syncytial virus (RSV) is an RNA virus that belongs to the Pneumovirus genus of the Paramyxoviridae family. The aim of this study was to evaluate the expressions of inducible nitric oxide synthetase (iNOS), CD3 (pan T cells), and CD8 (cytotoxic T cells) in lamb lungs naturally infected with RSV using immunohistochemistry (IHC). For this purpose, 100 pneumonic and 10 control lung tissue samples were taken from lambs slaughtered in the slaughterhouse after macroscopic examination. The streptavidin– peroxidase method (ABC) was used for IHC staining, and it revealed RSV positivity in 18 of 100 examined lungs with pneumonia (18%). These positive cases were then immunostained for iNOS, CD3, and CD8, and compared to controls. In all these cases, an increase in iNOS expression (100%) was detected, the higher number of CD3+ T lymphocytes was detected in 14 (78%) cases while CD8+ T lymphocytes were detected in five (28%) cases, only. Given the increase of iNOS immunoexpression in all RSV-positive cases and increase in the number of CD3+ T lymphocytes in most cases, it was concluded that iNOS and CD3+ T lymphocytes play an important role in the immune response in lamb pneumonia with naturally occurring RSV infection. With this study, the role of the mentioned markers was evaluated for the first time in lambs naturally infected with RSV.Yaman TuranAydemir CeyhunSciendoarticlecd3cd8immunohistochemistryinoslambrsvVeterinary medicineSF600-1100ENActa Veterinaria, Vol 71, Iss 2, Pp 170-188 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cd3 cd8 immunohistochemistry inos lamb rsv Veterinary medicine SF600-1100
spellingShingle	cd3 cd8 immunohistochemistry inos lamb rsv Veterinary medicine SF600-1100 Yaman Turan Aydemir Ceyhun Respiratory Syncytial Virus Infection Induces Expression of Inducible Nitric Oxide Synthase, CD3, and CD8 in Naturally Occurring Pneumonia in Lambs
description	Respiratory syncytial virus (RSV) is an RNA virus that belongs to the Pneumovirus genus of the Paramyxoviridae family. The aim of this study was to evaluate the expressions of inducible nitric oxide synthetase (iNOS), CD3 (pan T cells), and CD8 (cytotoxic T cells) in lamb lungs naturally infected with RSV using immunohistochemistry (IHC). For this purpose, 100 pneumonic and 10 control lung tissue samples were taken from lambs slaughtered in the slaughterhouse after macroscopic examination. The streptavidin– peroxidase method (ABC) was used for IHC staining, and it revealed RSV positivity in 18 of 100 examined lungs with pneumonia (18%). These positive cases were then immunostained for iNOS, CD3, and CD8, and compared to controls. In all these cases, an increase in iNOS expression (100%) was detected, the higher number of CD3+ T lymphocytes was detected in 14 (78%) cases while CD8+ T lymphocytes were detected in five (28%) cases, only. Given the increase of iNOS immunoexpression in all RSV-positive cases and increase in the number of CD3+ T lymphocytes in most cases, it was concluded that iNOS and CD3+ T lymphocytes play an important role in the immune response in lamb pneumonia with naturally occurring RSV infection. With this study, the role of the mentioned markers was evaluated for the first time in lambs naturally infected with RSV.
format	article
author	Yaman Turan Aydemir Ceyhun
author_facet	Yaman Turan Aydemir Ceyhun
author_sort	Yaman Turan
title	Respiratory Syncytial Virus Infection Induces Expression of Inducible Nitric Oxide Synthase, CD3, and CD8 in Naturally Occurring Pneumonia in Lambs
title_short	Respiratory Syncytial Virus Infection Induces Expression of Inducible Nitric Oxide Synthase, CD3, and CD8 in Naturally Occurring Pneumonia in Lambs
title_full	Respiratory Syncytial Virus Infection Induces Expression of Inducible Nitric Oxide Synthase, CD3, and CD8 in Naturally Occurring Pneumonia in Lambs
title_fullStr	Respiratory Syncytial Virus Infection Induces Expression of Inducible Nitric Oxide Synthase, CD3, and CD8 in Naturally Occurring Pneumonia in Lambs
title_full_unstemmed	Respiratory Syncytial Virus Infection Induces Expression of Inducible Nitric Oxide Synthase, CD3, and CD8 in Naturally Occurring Pneumonia in Lambs
title_sort	respiratory syncytial virus infection induces expression of inducible nitric oxide synthase, cd3, and cd8 in naturally occurring pneumonia in lambs
publisher	Sciendo
publishDate	2021
url	https://doaj.org/article/f5225c94a3cd44e2a8d9410f2612478d
work_keys_str_mv	AT yamanturan respiratorysyncytialvirusinfectioninducesexpressionofinduciblenitricoxidesynthasecd3andcd8innaturallyoccurringpneumoniainlambs AT aydemirceyhun respiratorysyncytialvirusinfectioninducesexpressionofinduciblenitricoxidesynthasecd3andcd8innaturallyoccurringpneumoniainlambs
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Respiratory Syncytial Virus Infection Induces Expression of Inducible Nitric Oxide Synthase, CD3, and CD8 in Naturally Occurring Pneumonia in Lambs

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