Gliogenic Potential of Single Pallial Radial Glial Cells in Lower Cortical Layers

During embryonic development, progenitor cells are progressively restricted in their potential to generate different neural cells. A specific progenitor cell type, the radial glial cells, divides symmetrically and then asymmetrically to produce neurons, astrocytes, oligodendrocytes, and NG2-glia in...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ana Cristina Ojalvo-Sanz, Laura López-Mascaraque
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>StarTrack</i> glial cell types lineage progenitor astrocytes oligodendroglia Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f5254ea9af2d4163ac2e31b95931dab9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f5254ea9af2d4163ac2e31b95931dab9
record_format	dspace
spelling	oai:doaj.org-article:f5254ea9af2d4163ac2e31b95931dab92021-11-25T17:13:11ZGliogenic Potential of Single Pallial Radial Glial Cells in Lower Cortical Layers10.3390/cells101132372073-4409https://doaj.org/article/f5254ea9af2d4163ac2e31b95931dab92021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3237https://doaj.org/toc/2073-4409During embryonic development, progenitor cells are progressively restricted in their potential to generate different neural cells. A specific progenitor cell type, the radial glial cells, divides symmetrically and then asymmetrically to produce neurons, astrocytes, oligodendrocytes, and NG2-glia in the cerebral cortex. However, the potential of individual progenitors to form glial lineages remains poorly understood. To further investigate the cell progeny of single pallial GFAP-expressing progenitors, we used the in vivo genetic lineage-tracing method, the <i>UbC-(GFAP-PB)-StarTrack</i>. After targeting those progenitors in embryonic mice brains, we tracked their adult glial progeny in lower cortical layers. Clonal analyses revealed the presence of clones containing sibling cells of either a glial cell type (uniform clones) or two different glial cell types (mixed clones). Further, the clonal size and rostro-caudal cell dispersion of sibling cells differed depending on the cell type. We concluded that pallial E14 neural progenitors are a heterogeneous cell population with respect to which glial cell type they produce, as well as the clonal size of their cell progeny.Ana Cristina Ojalvo-SanzLaura López-MascaraqueMDPI AGarticle<i>StarTrack</i>glial cell typeslineageprogenitorastrocytesoligodendrogliaBiology (General)QH301-705.5ENCells, Vol 10, Iss 3237, p 3237 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>StarTrack</i> glial cell types lineage progenitor astrocytes oligodendroglia Biology (General) QH301-705.5
spellingShingle	<i>StarTrack</i> glial cell types lineage progenitor astrocytes oligodendroglia Biology (General) QH301-705.5 Ana Cristina Ojalvo-Sanz Laura López-Mascaraque Gliogenic Potential of Single Pallial Radial Glial Cells in Lower Cortical Layers
description	During embryonic development, progenitor cells are progressively restricted in their potential to generate different neural cells. A specific progenitor cell type, the radial glial cells, divides symmetrically and then asymmetrically to produce neurons, astrocytes, oligodendrocytes, and NG2-glia in the cerebral cortex. However, the potential of individual progenitors to form glial lineages remains poorly understood. To further investigate the cell progeny of single pallial GFAP-expressing progenitors, we used the in vivo genetic lineage-tracing method, the <i>UbC-(GFAP-PB)-StarTrack</i>. After targeting those progenitors in embryonic mice brains, we tracked their adult glial progeny in lower cortical layers. Clonal analyses revealed the presence of clones containing sibling cells of either a glial cell type (uniform clones) or two different glial cell types (mixed clones). Further, the clonal size and rostro-caudal cell dispersion of sibling cells differed depending on the cell type. We concluded that pallial E14 neural progenitors are a heterogeneous cell population with respect to which glial cell type they produce, as well as the clonal size of their cell progeny.
format	article
author	Ana Cristina Ojalvo-Sanz Laura López-Mascaraque
author_facet	Ana Cristina Ojalvo-Sanz Laura López-Mascaraque
author_sort	Ana Cristina Ojalvo-Sanz
title	Gliogenic Potential of Single Pallial Radial Glial Cells in Lower Cortical Layers
title_short	Gliogenic Potential of Single Pallial Radial Glial Cells in Lower Cortical Layers
title_full	Gliogenic Potential of Single Pallial Radial Glial Cells in Lower Cortical Layers
title_fullStr	Gliogenic Potential of Single Pallial Radial Glial Cells in Lower Cortical Layers
title_full_unstemmed	Gliogenic Potential of Single Pallial Radial Glial Cells in Lower Cortical Layers
title_sort	gliogenic potential of single pallial radial glial cells in lower cortical layers
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f5254ea9af2d4163ac2e31b95931dab9
work_keys_str_mv	AT anacristinaojalvosanz gliogenicpotentialofsinglepallialradialglialcellsinlowercorticallayers AT lauralopezmascaraque gliogenicpotentialofsinglepallialradialglialcellsinlowercorticallayers
_version_	1718412626242830336

Gliogenic Potential of Single Pallial Radial Glial Cells in Lower Cortical Layers

Ejemplares similares