Lack of parent-of-origin effects in Nasonia jewel wasp: A replication and extension study.

In diploid cells, the paternal and maternal alleles are, on average, equally expressed. There are exceptions from this: a small number of genes express the maternal or paternal allele copy exclusively. This phenomenon, known as genomic imprinting, is common among eutherian mammals and some plant spe...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kimberly C Olney, Joshua D Gibson, Heini M Natri, Avery Underwood, Juergen Gadau, Melissa A Wilson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f53b5e11348b44839fdb9ea725c65c0e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f53b5e11348b44839fdb9ea725c65c0e
record_format	dspace
spelling	oai:doaj.org-article:f53b5e11348b44839fdb9ea725c65c0e2021-12-02T20:10:51ZLack of parent-of-origin effects in Nasonia jewel wasp: A replication and extension study.1932-620310.1371/journal.pone.0252457https://doaj.org/article/f53b5e11348b44839fdb9ea725c65c0e2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0252457https://doaj.org/toc/1932-6203In diploid cells, the paternal and maternal alleles are, on average, equally expressed. There are exceptions from this: a small number of genes express the maternal or paternal allele copy exclusively. This phenomenon, known as genomic imprinting, is common among eutherian mammals and some plant species; however, genomic imprinting in species with haplodiploid sex determination is not well characterized. Previous work reported no parent-of-origin effects in the hybrids of closely related haplodiploid Nasonia vitripennis and Nasonia giraulti jewel wasps, suggesting a lack of epigenetic reprogramming during embryogenesis in these species. Here, we replicate the gene expression dataset and observations using different individuals and sequencing technology, as well as reproduce these findings using the previously published RNA sequence data following our data analysis strategy. The major difference from the previous dataset is that they used an introgression strain as one of the parents and we found several loci that resisted introgression in that strain. Our results from both datasets demonstrate a species-of-origin effect, rather than a parent-of-origin effect. We present a reproducible workflow that others may use for replicating the results. Overall, we reproduced the original report of no parent-of-origin effects in the haplodiploid Nasonia using the original data with our new processing and analysis pipeline and replicated these results with our newly generated data.Kimberly C OlneyJoshua D GibsonHeini M NatriAvery UnderwoodJuergen GadauMelissa A WilsonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0252457 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kimberly C Olney Joshua D Gibson Heini M Natri Avery Underwood Juergen Gadau Melissa A Wilson Lack of parent-of-origin effects in Nasonia jewel wasp: A replication and extension study.
description	In diploid cells, the paternal and maternal alleles are, on average, equally expressed. There are exceptions from this: a small number of genes express the maternal or paternal allele copy exclusively. This phenomenon, known as genomic imprinting, is common among eutherian mammals and some plant species; however, genomic imprinting in species with haplodiploid sex determination is not well characterized. Previous work reported no parent-of-origin effects in the hybrids of closely related haplodiploid Nasonia vitripennis and Nasonia giraulti jewel wasps, suggesting a lack of epigenetic reprogramming during embryogenesis in these species. Here, we replicate the gene expression dataset and observations using different individuals and sequencing technology, as well as reproduce these findings using the previously published RNA sequence data following our data analysis strategy. The major difference from the previous dataset is that they used an introgression strain as one of the parents and we found several loci that resisted introgression in that strain. Our results from both datasets demonstrate a species-of-origin effect, rather than a parent-of-origin effect. We present a reproducible workflow that others may use for replicating the results. Overall, we reproduced the original report of no parent-of-origin effects in the haplodiploid Nasonia using the original data with our new processing and analysis pipeline and replicated these results with our newly generated data.
format	article
author	Kimberly C Olney Joshua D Gibson Heini M Natri Avery Underwood Juergen Gadau Melissa A Wilson
author_facet	Kimberly C Olney Joshua D Gibson Heini M Natri Avery Underwood Juergen Gadau Melissa A Wilson
author_sort	Kimberly C Olney
title	Lack of parent-of-origin effects in Nasonia jewel wasp: A replication and extension study.
title_short	Lack of parent-of-origin effects in Nasonia jewel wasp: A replication and extension study.
title_full	Lack of parent-of-origin effects in Nasonia jewel wasp: A replication and extension study.
title_fullStr	Lack of parent-of-origin effects in Nasonia jewel wasp: A replication and extension study.
title_full_unstemmed	Lack of parent-of-origin effects in Nasonia jewel wasp: A replication and extension study.
title_sort	lack of parent-of-origin effects in nasonia jewel wasp: a replication and extension study.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/f53b5e11348b44839fdb9ea725c65c0e
work_keys_str_mv	AT kimberlycolney lackofparentoforigineffectsinnasoniajewelwaspareplicationandextensionstudy AT joshuadgibson lackofparentoforigineffectsinnasoniajewelwaspareplicationandextensionstudy AT heinimnatri lackofparentoforigineffectsinnasoniajewelwaspareplicationandextensionstudy AT averyunderwood lackofparentoforigineffectsinnasoniajewelwaspareplicationandextensionstudy AT juergengadau lackofparentoforigineffectsinnasoniajewelwaspareplicationandextensionstudy AT melissaawilson lackofparentoforigineffectsinnasoniajewelwaspareplicationandextensionstudy
_version_	1718374980811489280

Lack of parent-of-origin effects in Nasonia jewel wasp: A replication and extension study.

Ejemplares similares