Multi-drug resistance and biofilm production among diarrheagenic Escherichia coli pathotypes isolated from stools of children with acute diarrheal disease

Background. Diarrheagenic E. coli (DEC) is an etiological agent of childhood diarrhea. Resistance against commonly used drugs in the empirical treatment of enteric infections has increased among DEC. Relationship between antibiotic resistance and biofilm formation in microorganisms have been widely...

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Autores principales:	E. E. David, M. A. Yameen, I. Igwenyi, A. C. Okafor, U. N. Obeten, D. O. Obasi, U. R. Ezeilo, M. N. Emeribole, C. N. David
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2021
Materias:	diarrheagenic e. coli (dec) antibiotic resistance diarrheal stool multiplex pcr multi-drug resistance polymerase chain reaction biofilms Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/f541ff01d7b945c98d3b707d221b8ef4
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spelling	oai:doaj.org-article:f541ff01d7b945c98d3b707d221b8ef42021-11-22T07:09:56ZMulti-drug resistance and biofilm production among diarrheagenic Escherichia coli pathotypes isolated from stools of children with acute diarrheal disease2220-76192313-739810.15789/2220-7619-MDR-1589https://doaj.org/article/f541ff01d7b945c98d3b707d221b8ef42021-11-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/1589https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Background. Diarrheagenic E. coli (DEC) is an etiological agent of childhood diarrhea. Resistance against commonly used drugs in the empirical treatment of enteric infections has increased among DEC. Relationship between antibiotic resistance and biofilm formation in microorganisms have been widely reported. This study was aimed to determine the antibiotic resistance and biofilm production pattern among DEC pathotypes isolated from stools of children aged 0–5 years with acute diarrheal disease in Abakaliki, Nigeria. Materials and methods. Diarrheal stool samples were obtained from 60 children and E. coli were isolated and identified using standard guidelines provided for laboratory diagnosis of enteric pathogens. Molecular identification was done by amplification of E. coli universal stress protein A (uspA) using polymerase chain reaction (PCR) method. Detection of virulent genes of DEC pathotypes was performed in a group of multiplex PCR using their specific primers. Kirby–Bauer disk diffusion method was used to determine the antibiotic susceptibility patterns of the isolates while biofilms production was detected by thiazolyl blue tetrazolium bromide dye in a 96-well plate. Results. DEC was isolated in 40 stools among which EIEC [40% (n = 16)] was commonly detected followed by ETEC [30% (n = 12)], EAEC [20% (n = 8)] and typical EPEC [10% (n = 4)]. Half of EAEC showed the highest multidrug resistance against ampicillin, cefoxitin, ciprofloxacin, levofloxacin, and tetracycline with the strongest biofilm production followed by all the EPEC which were resistant to ampicillin, ciprofloxacin, levofloxacin, and tetracycline with moderate biofilm production. All the LT-ETEC exhibited the least resistance to ampicillin and tetracycline with the weakest biofilm production. Conclusion. High frequency of the EIEC pathotype suggests its role as the primary etiological agent of diarrhea in children. Correlation between high drug resistance and biofilm production among the pathotype may indicate that biofilms may provide compatible uptake of resistance genes.E. E. DavidM. A. YameenI. IgwenyiA. C. OkaforU. N. ObetenD. O. ObasiU. R. EzeiloM. N. EmeriboleC. N. DavidSankt-Peterburg : NIIÈM imeni Pasteraarticlediarrheagenic e. coli (dec)antibiotic resistancediarrheal stoolmultiplex pcrmulti-drug resistancepolymerase chain reactionbiofilmsInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 11, Iss 5, Pp 958-964 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	diarrheagenic e. coli (dec) antibiotic resistance diarrheal stool multiplex pcr multi-drug resistance polymerase chain reaction biofilms Infectious and parasitic diseases RC109-216
spellingShingle	diarrheagenic e. coli (dec) antibiotic resistance diarrheal stool multiplex pcr multi-drug resistance polymerase chain reaction biofilms Infectious and parasitic diseases RC109-216 E. E. David M. A. Yameen I. Igwenyi A. C. Okafor U. N. Obeten D. O. Obasi U. R. Ezeilo M. N. Emeribole C. N. David Multi-drug resistance and biofilm production among diarrheagenic Escherichia coli pathotypes isolated from stools of children with acute diarrheal disease
description	Background. Diarrheagenic E. coli (DEC) is an etiological agent of childhood diarrhea. Resistance against commonly used drugs in the empirical treatment of enteric infections has increased among DEC. Relationship between antibiotic resistance and biofilm formation in microorganisms have been widely reported. This study was aimed to determine the antibiotic resistance and biofilm production pattern among DEC pathotypes isolated from stools of children aged 0–5 years with acute diarrheal disease in Abakaliki, Nigeria. Materials and methods. Diarrheal stool samples were obtained from 60 children and E. coli were isolated and identified using standard guidelines provided for laboratory diagnosis of enteric pathogens. Molecular identification was done by amplification of E. coli universal stress protein A (uspA) using polymerase chain reaction (PCR) method. Detection of virulent genes of DEC pathotypes was performed in a group of multiplex PCR using their specific primers. Kirby–Bauer disk diffusion method was used to determine the antibiotic susceptibility patterns of the isolates while biofilms production was detected by thiazolyl blue tetrazolium bromide dye in a 96-well plate. Results. DEC was isolated in 40 stools among which EIEC [40% (n = 16)] was commonly detected followed by ETEC [30% (n = 12)], EAEC [20% (n = 8)] and typical EPEC [10% (n = 4)]. Half of EAEC showed the highest multidrug resistance against ampicillin, cefoxitin, ciprofloxacin, levofloxacin, and tetracycline with the strongest biofilm production followed by all the EPEC which were resistant to ampicillin, ciprofloxacin, levofloxacin, and tetracycline with moderate biofilm production. All the LT-ETEC exhibited the least resistance to ampicillin and tetracycline with the weakest biofilm production. Conclusion. High frequency of the EIEC pathotype suggests its role as the primary etiological agent of diarrhea in children. Correlation between high drug resistance and biofilm production among the pathotype may indicate that biofilms may provide compatible uptake of resistance genes.
format	article
author	E. E. David M. A. Yameen I. Igwenyi A. C. Okafor U. N. Obeten D. O. Obasi U. R. Ezeilo M. N. Emeribole C. N. David
author_facet	E. E. David M. A. Yameen I. Igwenyi A. C. Okafor U. N. Obeten D. O. Obasi U. R. Ezeilo M. N. Emeribole C. N. David
author_sort	E. E. David
title	Multi-drug resistance and biofilm production among diarrheagenic Escherichia coli pathotypes isolated from stools of children with acute diarrheal disease
title_short	Multi-drug resistance and biofilm production among diarrheagenic Escherichia coli pathotypes isolated from stools of children with acute diarrheal disease
title_full	Multi-drug resistance and biofilm production among diarrheagenic Escherichia coli pathotypes isolated from stools of children with acute diarrheal disease
title_fullStr	Multi-drug resistance and biofilm production among diarrheagenic Escherichia coli pathotypes isolated from stools of children with acute diarrheal disease
title_full_unstemmed	Multi-drug resistance and biofilm production among diarrheagenic Escherichia coli pathotypes isolated from stools of children with acute diarrheal disease
title_sort	multi-drug resistance and biofilm production among diarrheagenic escherichia coli pathotypes isolated from stools of children with acute diarrheal disease
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2021
url	https://doaj.org/article/f541ff01d7b945c98d3b707d221b8ef4
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Multi-drug resistance and biofilm production among diarrheagenic Escherichia coli pathotypes isolated from stools of children with acute diarrheal disease

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