Functional insights into recombinant TROSPA protein from Ixodes ricinus.

Lyme disease (also called borreliosis) is a prevalent chronic disease transmitted by ticks and caused by Borrelia burgdorferi s. l. spirochete. At least one tick protein, namely TROSPA from I. scapularis, commonly occurring in the USA, was shown to be required for colonization of the vector by bacte...

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Autores principales:	Marek Figlerowicz, Anna Urbanowicz, Dominik Lewandowski, Jadwiga Jodynis-Liebert, Czeslaw Sadowski
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f5508d101e8544459fa163c6ff56bd1c
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spelling	oai:doaj.org-article:f5508d101e8544459fa163c6ff56bd1c2021-11-18T08:50:25ZFunctional insights into recombinant TROSPA protein from Ixodes ricinus.1932-620310.1371/journal.pone.0076848https://doaj.org/article/f5508d101e8544459fa163c6ff56bd1c2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24204685/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Lyme disease (also called borreliosis) is a prevalent chronic disease transmitted by ticks and caused by Borrelia burgdorferi s. l. spirochete. At least one tick protein, namely TROSPA from I. scapularis, commonly occurring in the USA, was shown to be required for colonization of the vector by bacteria. Located in the tick gut, TROSPA interacts with the spirochete outer surface protein A (OspA) and initiates the tick colonization. Ixodes ricinus is a primary vector involved in B. burgdorferi s. l. transmission in most European countries. In this study, we characterized the capacities of recombinant TROSPA protein from I. ricinus to interact with OspA from different Borrelia species and to induce an immune response in animals. We also showed that the N-terminal part of TROSPA (a putative transmembrane domain) is not involved in the interaction with OspA and that reduction of the total negative charge on the TROSPA protein impaired TROSPA-OspA binding. In general, the data presented in this paper indicate that recombinant TROSPA protein retains the capacity to form a complex with OspA and induces a significant level of IgG in orally immunized rats. Thus, I. ricinus TROSPA may be considered a good candidate component for an animal vaccine against Borrelia.Marek FiglerowiczAnna UrbanowiczDominik LewandowskiJadwiga Jodynis-LiebertCzeslaw SadowskiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 10, p e76848 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marek Figlerowicz Anna Urbanowicz Dominik Lewandowski Jadwiga Jodynis-Liebert Czeslaw Sadowski Functional insights into recombinant TROSPA protein from Ixodes ricinus.
description	Lyme disease (also called borreliosis) is a prevalent chronic disease transmitted by ticks and caused by Borrelia burgdorferi s. l. spirochete. At least one tick protein, namely TROSPA from I. scapularis, commonly occurring in the USA, was shown to be required for colonization of the vector by bacteria. Located in the tick gut, TROSPA interacts with the spirochete outer surface protein A (OspA) and initiates the tick colonization. Ixodes ricinus is a primary vector involved in B. burgdorferi s. l. transmission in most European countries. In this study, we characterized the capacities of recombinant TROSPA protein from I. ricinus to interact with OspA from different Borrelia species and to induce an immune response in animals. We also showed that the N-terminal part of TROSPA (a putative transmembrane domain) is not involved in the interaction with OspA and that reduction of the total negative charge on the TROSPA protein impaired TROSPA-OspA binding. In general, the data presented in this paper indicate that recombinant TROSPA protein retains the capacity to form a complex with OspA and induces a significant level of IgG in orally immunized rats. Thus, I. ricinus TROSPA may be considered a good candidate component for an animal vaccine against Borrelia.
format	article
author	Marek Figlerowicz Anna Urbanowicz Dominik Lewandowski Jadwiga Jodynis-Liebert Czeslaw Sadowski
author_facet	Marek Figlerowicz Anna Urbanowicz Dominik Lewandowski Jadwiga Jodynis-Liebert Czeslaw Sadowski
author_sort	Marek Figlerowicz
title	Functional insights into recombinant TROSPA protein from Ixodes ricinus.
title_short	Functional insights into recombinant TROSPA protein from Ixodes ricinus.
title_full	Functional insights into recombinant TROSPA protein from Ixodes ricinus.
title_fullStr	Functional insights into recombinant TROSPA protein from Ixodes ricinus.
title_full_unstemmed	Functional insights into recombinant TROSPA protein from Ixodes ricinus.
title_sort	functional insights into recombinant trospa protein from ixodes ricinus.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/f5508d101e8544459fa163c6ff56bd1c
work_keys_str_mv	AT marekfiglerowicz functionalinsightsintorecombinanttrospaproteinfromixodesricinus AT annaurbanowicz functionalinsightsintorecombinanttrospaproteinfromixodesricinus AT dominiklewandowski functionalinsightsintorecombinanttrospaproteinfromixodesricinus AT jadwigajodynisliebert functionalinsightsintorecombinanttrospaproteinfromixodesricinus AT czeslawsadowski functionalinsightsintorecombinanttrospaproteinfromixodesricinus
_version_	1718421299583254528

Functional insights into recombinant TROSPA protein from Ixodes ricinus.

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