Silver nanoparticles: aggregation behavior in biorelevant conditions and its impact on biological activity

Péter Bélteky,1 Andrea Rónavári,1,2 Nóra Igaz,2 Bettina Szerencsés,3 Ildikó Y Tóth,1 Ilona Pfeiffer,3 Mónika Kiricsi,2 Zoltán Kónya1,4 1Department of Applied and Environmental Ch...

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Autores principales:	Bélteky P, Rónavári A, Igaz N, Szerencsés B, Tóth IY, Pfeiffer I, Kiricsi M, Kónya Z
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Colloidal stability green synthesis antimicrobial activity cytotoxicity Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f554c7ff2c11457dbc30ab29edb37734
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spelling	oai:doaj.org-article:f554c7ff2c11457dbc30ab29edb377342021-12-02T02:02:16ZSilver nanoparticles: aggregation behavior in biorelevant conditions and its impact on biological activity1178-2013https://doaj.org/article/f554c7ff2c11457dbc30ab29edb377342019-01-01T00:00:00Zhttps://www.dovepress.com/silver-nanoparticles-aggregation-behavior-in-biorelevant-conditions-an-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Péter Bélteky,1 Andrea Rónavári,1,2 Nóra Igaz,2 Bettina Szerencsés,3 Ildikó Y Tóth,1 Ilona Pfeiffer,3 Mónika Kiricsi,2 Zoltán Kónya1,4 1Department of Applied and Environmental Chemistry, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary; 2Department of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary; 3Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary; 4MTA-SZTE Reaction Kinetics and Surface Chemistry Research Group, Szeged, Hungary Purpose: The biomedical applications of silver nanoparticles (AgNPs) are heavily investigated due to their cytotoxic and antimicrobial properties. However, the scientific literature is lacking in data on the aggregation behavior of nanoparticles, especially regarding its impact on biological activity. Therefore, to assess the potential of AgNPs in therapeutic applications, two different AgNP samples were compared under biorelevant conditions.Methods: Citrate-capped nanosilver was produced by classical chemical reduction and stabilization with sodium citrate (AgNP@C), while green tea extract was used to produce silver nanoparticles in a green synthesis approach (AgNP@GTs). Particle size, morphology, and crystallinity were characterized using transmission electron microscopy. To observe the effects of the most important biorelevant conditions on AgNP colloidal stability, aggregation grade measurements were carried out using UV-Vis spectroscopy and dynamic light scatterig, while MTT assay and a microdilution method were performed to evaluate the effects of aggregation on cytotoxicity and antimicrobial activity in a time-dependent manner.Results: The aggregation behavior of AgNPs is mostly affected by pH and electrolyte concentration, while the presence of biomolecules can improve particle stability due to the biomolecular corona effect. We demonstrated that high aggregation grade in both AgNP samples attenuated their toxic effect toward living cells. However, AgNP@GT proved less prone to aggregation thus retained a degree of its toxicity.Conclusion: To our knowledge, this is the first systematic examination regarding AgNP aggregation behavior with simultaneous measurements of its effect on biological activity. We showed that nanoparticle behavior in complex systems can be estimated by simple compounds like sodium chloride and glutamine. Electrostatic stabilization might not be suitable for biomedical AgNP applications, while green synthesis approaches could offer new frontiers to preserve nanoparticle toxicity by enhancing colloidal stability. The importance of properly selected synthesis methods must be emphasized as they profoundly influence colloidal stability, and therefore biological activity. Keywords: colloidal stability, green synthesis, antimicrobial activity, cytotoxicity  Bélteky PRónavári AIgaz NSzerencsés BTóth IYPfeiffer IKiricsi MKónya ZDove Medical PressarticleColloidal stabilitygreen synthesisantimicrobial activitycytotoxicityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 667-687 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Colloidal stability green synthesis antimicrobial activity cytotoxicity Medicine (General) R5-920
spellingShingle	Colloidal stability green synthesis antimicrobial activity cytotoxicity Medicine (General) R5-920 Bélteky P Rónavári A Igaz N Szerencsés B Tóth IY Pfeiffer I Kiricsi M Kónya Z Silver nanoparticles: aggregation behavior in biorelevant conditions and its impact on biological activity
description	Péter Bélteky,1 Andrea Rónavári,1,2 Nóra Igaz,2 Bettina Szerencsés,3 Ildikó Y Tóth,1 Ilona Pfeiffer,3 Mónika Kiricsi,2 Zoltán Kónya1,4 1Department of Applied and Environmental Chemistry, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary; 2Department of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary; 3Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary; 4MTA-SZTE Reaction Kinetics and Surface Chemistry Research Group, Szeged, Hungary Purpose: The biomedical applications of silver nanoparticles (AgNPs) are heavily investigated due to their cytotoxic and antimicrobial properties. However, the scientific literature is lacking in data on the aggregation behavior of nanoparticles, especially regarding its impact on biological activity. Therefore, to assess the potential of AgNPs in therapeutic applications, two different AgNP samples were compared under biorelevant conditions.Methods: Citrate-capped nanosilver was produced by classical chemical reduction and stabilization with sodium citrate (AgNP@C), while green tea extract was used to produce silver nanoparticles in a green synthesis approach (AgNP@GTs). Particle size, morphology, and crystallinity were characterized using transmission electron microscopy. To observe the effects of the most important biorelevant conditions on AgNP colloidal stability, aggregation grade measurements were carried out using UV-Vis spectroscopy and dynamic light scatterig, while MTT assay and a microdilution method were performed to evaluate the effects of aggregation on cytotoxicity and antimicrobial activity in a time-dependent manner.Results: The aggregation behavior of AgNPs is mostly affected by pH and electrolyte concentration, while the presence of biomolecules can improve particle stability due to the biomolecular corona effect. We demonstrated that high aggregation grade in both AgNP samples attenuated their toxic effect toward living cells. However, AgNP@GT proved less prone to aggregation thus retained a degree of its toxicity.Conclusion: To our knowledge, this is the first systematic examination regarding AgNP aggregation behavior with simultaneous measurements of its effect on biological activity. We showed that nanoparticle behavior in complex systems can be estimated by simple compounds like sodium chloride and glutamine. Electrostatic stabilization might not be suitable for biomedical AgNP applications, while green synthesis approaches could offer new frontiers to preserve nanoparticle toxicity by enhancing colloidal stability. The importance of properly selected synthesis methods must be emphasized as they profoundly influence colloidal stability, and therefore biological activity. Keywords: colloidal stability, green synthesis, antimicrobial activity, cytotoxicity
format	article
author	Bélteky P Rónavári A Igaz N Szerencsés B Tóth IY Pfeiffer I Kiricsi M Kónya Z
author_facet	Bélteky P Rónavári A Igaz N Szerencsés B Tóth IY Pfeiffer I Kiricsi M Kónya Z
author_sort	Bélteky P
title	Silver nanoparticles: aggregation behavior in biorelevant conditions and its impact on biological activity
title_short	Silver nanoparticles: aggregation behavior in biorelevant conditions and its impact on biological activity
title_full	Silver nanoparticles: aggregation behavior in biorelevant conditions and its impact on biological activity
title_fullStr	Silver nanoparticles: aggregation behavior in biorelevant conditions and its impact on biological activity
title_full_unstemmed	Silver nanoparticles: aggregation behavior in biorelevant conditions and its impact on biological activity
title_sort	silver nanoparticles: aggregation behavior in biorelevant conditions and its impact on biological activity
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/f554c7ff2c11457dbc30ab29edb37734
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Silver nanoparticles: aggregation behavior in biorelevant conditions and its impact on biological activity

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