Apoptosis inducing ability of silver decorated highly reduced graphene oxide nanocomposites in A549 lung cancer

Merajuddin Khan,1 Mujeeb Khan,1 Abdulhadi H Al-Marri,1 Abdulrahman Al-Warthan,1 Hamad Z Alkhathlan,1 Mohammed Rafiq H Siddiqui,1 Vadithe Lakshma Nayak,2 Ahmed Kamal,2 Syed F Adil1 1Department of Chemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia; 2Department of Medi...

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Autores principales:	Khan M, Al-Marri AH, Al-Warthan A, Alkhathlan HZ, Siddiqui MR, Nayak VL, Kamal A, Adil SF
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	plant extract graphene/silver nanocomposites anticancer apoptosis. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f556fcdaf250491d9b9d59d22cf58c0d
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spelling	oai:doaj.org-article:f556fcdaf250491d9b9d59d22cf58c0d2021-12-02T07:21:41ZApoptosis inducing ability of silver decorated highly reduced graphene oxide nanocomposites in A549 lung cancer1178-2013https://doaj.org/article/f556fcdaf250491d9b9d59d22cf58c0d2016-03-01T00:00:00Zhttps://www.dovepress.com/apoptosis-inducing-ability-of-silver-decorated-highly-reduced-graphene-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Merajuddin Khan,1 Mujeeb Khan,1 Abdulhadi H Al-Marri,1 Abdulrahman Al-Warthan,1 Hamad Z Alkhathlan,1 Mohammed Rafiq H Siddiqui,1 Vadithe Lakshma Nayak,2 Ahmed Kamal,2 Syed F Adil1 1Department of Chemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia; 2Department of Medicinal Chemistry and Pharmacology, CSIR – Indian Institute of Chemical Technology, Hyderabad, India Abstract: Recently, graphene and graphene-based materials have been increasingly used for various biological applications due to their extraordinary physicochemical properties. Here, we demonstrate the anticancer properties and apoptosis-inducing ability of silver doped highly reduced graphene oxide nanocomposites synthesized by employing green approach. These nanocomposites (PGE-HRG-Ag) were synthesized by using Pulicaria glutinosa extract (PGE) as a reducing agent and were evaluated for their anticancer properties against various human cancer cell lines with tamoxifen as the reference drug. A correlation between the amount of Ag nanoparticles on the surface of highly reduced graphene oxide (HRG) and the anticancer activity of nanocomposite was observed, wherein an increase in the concentration of Ag nanoparticles on the surface of HRG led to the enhanced anticancer activity of the nanocomposite. The nanocomposite PGE-HRG-Ag-2 exhibited more potent cytotoxicity than standard drug in A549 cells, a human lung cancer cell line. A detailed investigation was undertaken and Fluorescence activated cell sorting (FACS) analysis demonstrated that the nanocomposite PGE-HRG-Ag-2 showed G0/G1 phase cell cycle arrest and induced apoptosis in A549 cells. Studies such as, measurement of mitochondrial membrane potential, generation of reactive oxygen species (ROS) and Annexin V-FITC staining assay suggested that this compound induced apoptosis in human lung cancer cells. Keywords: plant extract, graphene/silver nanocomposites, anticancer, apoptosisKhan MKhan MAl-Marri AHAl-Warthan AAlkhathlan HZSiddiqui MRNayak VLKamal AAdil SFDove Medical Pressarticleplant extractgraphene/silver nanocompositesanticancerapoptosis.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss Issue 1, Pp 873-883 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	plant extract graphene/silver nanocomposites anticancer apoptosis. Medicine (General) R5-920
spellingShingle	plant extract graphene/silver nanocomposites anticancer apoptosis. Medicine (General) R5-920 Khan M Khan M Al-Marri AH Al-Warthan A Alkhathlan HZ Siddiqui MR Nayak VL Kamal A Adil SF Apoptosis inducing ability of silver decorated highly reduced graphene oxide nanocomposites in A549 lung cancer
description	Merajuddin Khan,1 Mujeeb Khan,1 Abdulhadi H Al-Marri,1 Abdulrahman Al-Warthan,1 Hamad Z Alkhathlan,1 Mohammed Rafiq H Siddiqui,1 Vadithe Lakshma Nayak,2 Ahmed Kamal,2 Syed F Adil1 1Department of Chemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia; 2Department of Medicinal Chemistry and Pharmacology, CSIR – Indian Institute of Chemical Technology, Hyderabad, India Abstract: Recently, graphene and graphene-based materials have been increasingly used for various biological applications due to their extraordinary physicochemical properties. Here, we demonstrate the anticancer properties and apoptosis-inducing ability of silver doped highly reduced graphene oxide nanocomposites synthesized by employing green approach. These nanocomposites (PGE-HRG-Ag) were synthesized by using Pulicaria glutinosa extract (PGE) as a reducing agent and were evaluated for their anticancer properties against various human cancer cell lines with tamoxifen as the reference drug. A correlation between the amount of Ag nanoparticles on the surface of highly reduced graphene oxide (HRG) and the anticancer activity of nanocomposite was observed, wherein an increase in the concentration of Ag nanoparticles on the surface of HRG led to the enhanced anticancer activity of the nanocomposite. The nanocomposite PGE-HRG-Ag-2 exhibited more potent cytotoxicity than standard drug in A549 cells, a human lung cancer cell line. A detailed investigation was undertaken and Fluorescence activated cell sorting (FACS) analysis demonstrated that the nanocomposite PGE-HRG-Ag-2 showed G0/G1 phase cell cycle arrest and induced apoptosis in A549 cells. Studies such as, measurement of mitochondrial membrane potential, generation of reactive oxygen species (ROS) and Annexin V-FITC staining assay suggested that this compound induced apoptosis in human lung cancer cells. Keywords: plant extract, graphene/silver nanocomposites, anticancer, apoptosis
format	article
author	Khan M Khan M Al-Marri AH Al-Warthan A Alkhathlan HZ Siddiqui MR Nayak VL Kamal A Adil SF
author_facet	Khan M Khan M Al-Marri AH Al-Warthan A Alkhathlan HZ Siddiqui MR Nayak VL Kamal A Adil SF
author_sort	Khan M
title	Apoptosis inducing ability of silver decorated highly reduced graphene oxide nanocomposites in A549 lung cancer
title_short	Apoptosis inducing ability of silver decorated highly reduced graphene oxide nanocomposites in A549 lung cancer
title_full	Apoptosis inducing ability of silver decorated highly reduced graphene oxide nanocomposites in A549 lung cancer
title_fullStr	Apoptosis inducing ability of silver decorated highly reduced graphene oxide nanocomposites in A549 lung cancer
title_full_unstemmed	Apoptosis inducing ability of silver decorated highly reduced graphene oxide nanocomposites in A549 lung cancer
title_sort	apoptosis inducing ability of silver decorated highly reduced graphene oxide nanocomposites in a549 lung cancer
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/f556fcdaf250491d9b9d59d22cf58c0d
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Apoptosis inducing ability of silver decorated highly reduced graphene oxide nanocomposites in A549 lung cancer

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