Extracranial 125I Seed Implantation Allows Non-invasive Stereotactic Radioablation of Hippocampal Adult Neurogenesis in Guinea Pigs

Adult hippocampal neurogenesis (AHN) is important for multiple cognitive functions. We sort to establish a minimal or non-invasive radiation approach to ablate AHN using guinea pigs as an animal model. 125I seeds with different radiation dosages (1.0, 0.8, 0.6, 0.3 mCi) were implanted unilaterally b...

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Autores principales:	Lily Wan, Rou-Jie Huang, Chen Yang, Jia-Qi Ai, Qian Zhou, Jiao-E Gong, Jian Li, Yun Zhang, Zhao-Hui Luo, Ewen Tu, Aihua Pan, Bo Xiao, Xiao-Xin Yan
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	adult neurogenesis brachytherapy iodine-125 seed immature neurons radiation encephalopathy Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/f5570af22ddb4951a2234c8216a121a6
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spelling	oai:doaj.org-article:f5570af22ddb4951a2234c8216a121a62021-12-01T19:42:25ZExtracranial 125I Seed Implantation Allows Non-invasive Stereotactic Radioablation of Hippocampal Adult Neurogenesis in Guinea Pigs1662-453X10.3389/fnins.2021.756658https://doaj.org/article/f5570af22ddb4951a2234c8216a121a62021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fnins.2021.756658/fullhttps://doaj.org/toc/1662-453XAdult hippocampal neurogenesis (AHN) is important for multiple cognitive functions. We sort to establish a minimal or non-invasive radiation approach to ablate AHN using guinea pigs as an animal model. 125I seeds with different radiation dosages (1.0, 0.8, 0.6, 0.3 mCi) were implanted unilaterally between the scalp and skull above the temporal lobe for 30 and 60 days, with the radiation effect on proliferating cells, immature neurons, and mature neurons in the hippocampal formation determined by assessment of immunolabeled (+) cells for Ki67, doublecortin (DCX), and neuron-specific nuclear antigen (NeuN), as well as Nissl stain cells. Spatially, the ablation effect of radiation occurred across the entire rostrocaudal and largely the dorsoventral dimensions of the hippocampus, evidenced by a loss of DCX+ cells in the subgranular zone (SGZ) of dentate gyrus (DG) in the ipsilateral relative to contralateral hemispheres in reference to the 125I seed implant. Quantitatively, Ki67+ and DCX+ cells at the SGZ in the dorsal hippocampus were reduced in all dosage groups at the two surviving time points, more significant in the ipsilateral than contralateral sides, relative to sham controls. NeuN+ neurons and Nissl-stained cells were reduced in the granule cell layer of DG and the stratum pyramidale of CA1 in the groups with 0.6-mCi radiation for 60 days and 1.0 mCi for 30 and 60 days. Minimal cranial trauma was observed in the groups with 0.3– 1.0-mCi radiation at 60 days. These results suggest that extracranial radiation with 125I seed implantation can be used to deplete HAN in a radioactivity-, duration-, and space-controllable manner, with a “non-invasive” stereotactic ablation achievable by using 125I seeds with relatively low radioactivity dosages.Lily WanLily WanRou-Jie HuangChen YangJia-Qi AiQian ZhouJiao-E GongJian LiYun ZhangZhao-Hui LuoEwen TuAihua PanBo XiaoXiao-Xin YanFrontiers Media S.A.articleadult neurogenesisbrachytherapyiodine-125 seedimmature neuronsradiation encephalopathyNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENFrontiers in Neuroscience, Vol 15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adult neurogenesis brachytherapy iodine-125 seed immature neurons radiation encephalopathy Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	adult neurogenesis brachytherapy iodine-125 seed immature neurons radiation encephalopathy Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Lily Wan Lily Wan Rou-Jie Huang Chen Yang Jia-Qi Ai Qian Zhou Jiao-E Gong Jian Li Yun Zhang Zhao-Hui Luo Ewen Tu Aihua Pan Bo Xiao Xiao-Xin Yan Extracranial 125I Seed Implantation Allows Non-invasive Stereotactic Radioablation of Hippocampal Adult Neurogenesis in Guinea Pigs
description	Adult hippocampal neurogenesis (AHN) is important for multiple cognitive functions. We sort to establish a minimal or non-invasive radiation approach to ablate AHN using guinea pigs as an animal model. 125I seeds with different radiation dosages (1.0, 0.8, 0.6, 0.3 mCi) were implanted unilaterally between the scalp and skull above the temporal lobe for 30 and 60 days, with the radiation effect on proliferating cells, immature neurons, and mature neurons in the hippocampal formation determined by assessment of immunolabeled (+) cells for Ki67, doublecortin (DCX), and neuron-specific nuclear antigen (NeuN), as well as Nissl stain cells. Spatially, the ablation effect of radiation occurred across the entire rostrocaudal and largely the dorsoventral dimensions of the hippocampus, evidenced by a loss of DCX+ cells in the subgranular zone (SGZ) of dentate gyrus (DG) in the ipsilateral relative to contralateral hemispheres in reference to the 125I seed implant. Quantitatively, Ki67+ and DCX+ cells at the SGZ in the dorsal hippocampus were reduced in all dosage groups at the two surviving time points, more significant in the ipsilateral than contralateral sides, relative to sham controls. NeuN+ neurons and Nissl-stained cells were reduced in the granule cell layer of DG and the stratum pyramidale of CA1 in the groups with 0.6-mCi radiation for 60 days and 1.0 mCi for 30 and 60 days. Minimal cranial trauma was observed in the groups with 0.3– 1.0-mCi radiation at 60 days. These results suggest that extracranial radiation with 125I seed implantation can be used to deplete HAN in a radioactivity-, duration-, and space-controllable manner, with a “non-invasive” stereotactic ablation achievable by using 125I seeds with relatively low radioactivity dosages.
format	article
author	Lily Wan Lily Wan Rou-Jie Huang Chen Yang Jia-Qi Ai Qian Zhou Jiao-E Gong Jian Li Yun Zhang Zhao-Hui Luo Ewen Tu Aihua Pan Bo Xiao Xiao-Xin Yan
author_facet	Lily Wan Lily Wan Rou-Jie Huang Chen Yang Jia-Qi Ai Qian Zhou Jiao-E Gong Jian Li Yun Zhang Zhao-Hui Luo Ewen Tu Aihua Pan Bo Xiao Xiao-Xin Yan
author_sort	Lily Wan
title	Extracranial 125I Seed Implantation Allows Non-invasive Stereotactic Radioablation of Hippocampal Adult Neurogenesis in Guinea Pigs
title_short	Extracranial 125I Seed Implantation Allows Non-invasive Stereotactic Radioablation of Hippocampal Adult Neurogenesis in Guinea Pigs
title_full	Extracranial 125I Seed Implantation Allows Non-invasive Stereotactic Radioablation of Hippocampal Adult Neurogenesis in Guinea Pigs
title_fullStr	Extracranial 125I Seed Implantation Allows Non-invasive Stereotactic Radioablation of Hippocampal Adult Neurogenesis in Guinea Pigs
title_full_unstemmed	Extracranial 125I Seed Implantation Allows Non-invasive Stereotactic Radioablation of Hippocampal Adult Neurogenesis in Guinea Pigs
title_sort	extracranial 125i seed implantation allows non-invasive stereotactic radioablation of hippocampal adult neurogenesis in guinea pigs
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/f5570af22ddb4951a2234c8216a121a6
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Extracranial 125I Seed Implantation Allows Non-invasive Stereotactic Radioablation of Hippocampal Adult Neurogenesis in Guinea Pigs

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