Co-regulation of cell polarization and migration by caveolar proteins PTRF/Cavin-1 and caveolin-1.

Caveolin-1 and caveolae are differentially polarized in migrating cells in various models, and caveolin-1 expression has been shown to quantitatively modulate cell migration. PTRF/cavin-1 is a cytoplasmic protein now established to be also necessary for caveola formation. Here we tested the effect o...

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Main Authors:	Michelle M Hill, Noor Huda Daud, Cho Sanda Aung, Dorothy Loo, Sally Martin, Samantha Murphy, Debra M Black, Rachael Barry, Fiona Simpson, Libin Liu, Paul F Pilch, John F Hancock, Marie-Odile Parat, Robert G Parton
Format:	article
Language:	EN
Published:	Public Library of Science (PLoS) 2012
Subjects:	Medicine R Science Q
Online Access:	https://doaj.org/article/f55dfb02b458465fb7e9fce071638f91
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spelling	oai:doaj.org-article:f55dfb02b458465fb7e9fce071638f912021-11-18T07:08:55ZCo-regulation of cell polarization and migration by caveolar proteins PTRF/Cavin-1 and caveolin-1.1932-620310.1371/journal.pone.0043041https://doaj.org/article/f55dfb02b458465fb7e9fce071638f912012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22912783/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Caveolin-1 and caveolae are differentially polarized in migrating cells in various models, and caveolin-1 expression has been shown to quantitatively modulate cell migration. PTRF/cavin-1 is a cytoplasmic protein now established to be also necessary for caveola formation. Here we tested the effect of PTRF expression on cell migration. Using fluorescence imaging, quantitative proteomics, and cell migration assays we show that PTRF/cavin-1 modulates cellular polarization, and the subcellular localization of Rac1 and caveolin-1 in migrating cells as well as PKCα caveola recruitment. PTRF/cavin-1 quantitatively reduced cell migration, and induced mesenchymal epithelial reversion. Similar to caveolin-1, the polarization of PTRF/cavin-1 was dependent on the migration mode. By selectively manipulating PTRF/cavin-1 and caveolin-1 expression (and therefore caveola formation) in multiple cell systems, we unveil caveola-independent functions for both proteins in cell migration.Michelle M HillNoor Huda DaudCho Sanda AungDorothy LooSally MartinSamantha MurphyDebra M BlackRachael BarryFiona SimpsonLibin LiuPaul F PilchJohn F HancockMarie-Odile ParatRobert G PartonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e43041 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Michelle M Hill Noor Huda Daud Cho Sanda Aung Dorothy Loo Sally Martin Samantha Murphy Debra M Black Rachael Barry Fiona Simpson Libin Liu Paul F Pilch John F Hancock Marie-Odile Parat Robert G Parton Co-regulation of cell polarization and migration by caveolar proteins PTRF/Cavin-1 and caveolin-1.
description	Caveolin-1 and caveolae are differentially polarized in migrating cells in various models, and caveolin-1 expression has been shown to quantitatively modulate cell migration. PTRF/cavin-1 is a cytoplasmic protein now established to be also necessary for caveola formation. Here we tested the effect of PTRF expression on cell migration. Using fluorescence imaging, quantitative proteomics, and cell migration assays we show that PTRF/cavin-1 modulates cellular polarization, and the subcellular localization of Rac1 and caveolin-1 in migrating cells as well as PKCα caveola recruitment. PTRF/cavin-1 quantitatively reduced cell migration, and induced mesenchymal epithelial reversion. Similar to caveolin-1, the polarization of PTRF/cavin-1 was dependent on the migration mode. By selectively manipulating PTRF/cavin-1 and caveolin-1 expression (and therefore caveola formation) in multiple cell systems, we unveil caveola-independent functions for both proteins in cell migration.
format	article
author	Michelle M Hill Noor Huda Daud Cho Sanda Aung Dorothy Loo Sally Martin Samantha Murphy Debra M Black Rachael Barry Fiona Simpson Libin Liu Paul F Pilch John F Hancock Marie-Odile Parat Robert G Parton
author_facet	Michelle M Hill Noor Huda Daud Cho Sanda Aung Dorothy Loo Sally Martin Samantha Murphy Debra M Black Rachael Barry Fiona Simpson Libin Liu Paul F Pilch John F Hancock Marie-Odile Parat Robert G Parton
author_sort	Michelle M Hill
title	Co-regulation of cell polarization and migration by caveolar proteins PTRF/Cavin-1 and caveolin-1.
title_short	Co-regulation of cell polarization and migration by caveolar proteins PTRF/Cavin-1 and caveolin-1.
title_full	Co-regulation of cell polarization and migration by caveolar proteins PTRF/Cavin-1 and caveolin-1.
title_fullStr	Co-regulation of cell polarization and migration by caveolar proteins PTRF/Cavin-1 and caveolin-1.
title_full_unstemmed	Co-regulation of cell polarization and migration by caveolar proteins PTRF/Cavin-1 and caveolin-1.
title_sort	co-regulation of cell polarization and migration by caveolar proteins ptrf/cavin-1 and caveolin-1.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/f55dfb02b458465fb7e9fce071638f91
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Co-regulation of cell polarization and migration by caveolar proteins PTRF/Cavin-1 and caveolin-1.

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