Handheld Co-Axial Bioprinting: Application to in situ surgical cartilage repair

Abstract Three-dimensional (3D) bioprinting is driving major innovations in the area of cartilage tissue engineering. Extrusion-based 3D bioprinting necessitates a phase change from a liquid bioink to a semi-solid crosslinked network achieved by a photo-initiated free radical polymerization reaction...

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Autores principales:	Serena Duchi, Carmine Onofrillo, Cathal D. O’Connell, Romane Blanchard, Cheryl Augustine, Anita F. Quigley, Robert M. I. Kapsa, Peter Pivonka, Gordon Wallace, Claudia Di Bella, Peter F. M. Choong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f5657d8bd4ef41ec85be4158e2f3b61b
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spelling	oai:doaj.org-article:f5657d8bd4ef41ec85be4158e2f3b61b2021-12-02T16:06:43ZHandheld Co-Axial Bioprinting: Application to in situ surgical cartilage repair10.1038/s41598-017-05699-x2045-2322https://doaj.org/article/f5657d8bd4ef41ec85be4158e2f3b61b2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05699-xhttps://doaj.org/toc/2045-2322Abstract Three-dimensional (3D) bioprinting is driving major innovations in the area of cartilage tissue engineering. Extrusion-based 3D bioprinting necessitates a phase change from a liquid bioink to a semi-solid crosslinked network achieved by a photo-initiated free radical polymerization reaction that is known to be cytotoxic. Therefore, the choice of the photocuring conditions has to be carefully addressed to generate a structure stiff enough to withstand the forces phisiologically applied on articular cartilage, while ensuring adequate cell survival for functional chondral repair. We recently developed a handheld 3D printer called “Biopen”. To progress towards translating this freeform biofabrication tool into clinical practice, we aimed to define the ideal bioprinting conditions that would deliver a scaffold with high cell viability and structural stiffness relevant for chondral repair. To fulfill those criteria, free radical cytotoxicity was confined by a co-axial Core/Shell separation. This system allowed the generation of Core/Shell GelMa/HAMa bioscaffolds with stiffness of 200KPa, achieved after only 10 seconds of exposure to 700 mW/cm2 of 365 nm UV-A, containing >90% viable stem cells that retained proliferative capacity. Overall, the Core/Shell handheld 3D bioprinting strategy enabled rapid generation of high modulus bioscaffolds with high cell viability, with potential for in situ surgical cartilage engineering.Serena DuchiCarmine OnofrilloCathal D. O’ConnellRomane BlanchardCheryl AugustineAnita F. QuigleyRobert M. I. KapsaPeter PivonkaGordon WallaceClaudia Di BellaPeter F. M. ChoongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Serena Duchi Carmine Onofrillo Cathal D. O’Connell Romane Blanchard Cheryl Augustine Anita F. Quigley Robert M. I. Kapsa Peter Pivonka Gordon Wallace Claudia Di Bella Peter F. M. Choong Handheld Co-Axial Bioprinting: Application to in situ surgical cartilage repair
description	Abstract Three-dimensional (3D) bioprinting is driving major innovations in the area of cartilage tissue engineering. Extrusion-based 3D bioprinting necessitates a phase change from a liquid bioink to a semi-solid crosslinked network achieved by a photo-initiated free radical polymerization reaction that is known to be cytotoxic. Therefore, the choice of the photocuring conditions has to be carefully addressed to generate a structure stiff enough to withstand the forces phisiologically applied on articular cartilage, while ensuring adequate cell survival for functional chondral repair. We recently developed a handheld 3D printer called “Biopen”. To progress towards translating this freeform biofabrication tool into clinical practice, we aimed to define the ideal bioprinting conditions that would deliver a scaffold with high cell viability and structural stiffness relevant for chondral repair. To fulfill those criteria, free radical cytotoxicity was confined by a co-axial Core/Shell separation. This system allowed the generation of Core/Shell GelMa/HAMa bioscaffolds with stiffness of 200KPa, achieved after only 10 seconds of exposure to 700 mW/cm2 of 365 nm UV-A, containing >90% viable stem cells that retained proliferative capacity. Overall, the Core/Shell handheld 3D bioprinting strategy enabled rapid generation of high modulus bioscaffolds with high cell viability, with potential for in situ surgical cartilage engineering.
format	article
author	Serena Duchi Carmine Onofrillo Cathal D. O’Connell Romane Blanchard Cheryl Augustine Anita F. Quigley Robert M. I. Kapsa Peter Pivonka Gordon Wallace Claudia Di Bella Peter F. M. Choong
author_facet	Serena Duchi Carmine Onofrillo Cathal D. O’Connell Romane Blanchard Cheryl Augustine Anita F. Quigley Robert M. I. Kapsa Peter Pivonka Gordon Wallace Claudia Di Bella Peter F. M. Choong
author_sort	Serena Duchi
title	Handheld Co-Axial Bioprinting: Application to in situ surgical cartilage repair
title_short	Handheld Co-Axial Bioprinting: Application to in situ surgical cartilage repair
title_full	Handheld Co-Axial Bioprinting: Application to in situ surgical cartilage repair
title_fullStr	Handheld Co-Axial Bioprinting: Application to in situ surgical cartilage repair
title_full_unstemmed	Handheld Co-Axial Bioprinting: Application to in situ surgical cartilage repair
title_sort	handheld co-axial bioprinting: application to in situ surgical cartilage repair
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f5657d8bd4ef41ec85be4158e2f3b61b
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Handheld Co-Axial Bioprinting: Application to in situ surgical cartilage repair

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