Rumen Fluid Metabolomics Analysis Associated with Feed Efficiency on Crossbred Steers

Abstract The rumen has a central role in the efficiency of digestion in ruminants. To identify potential differences in rumen function that lead to differences in average daily gain (ADG), rumen fluid metabolomic analysis by LC-MS and multivariate/univariate statistical analysis were used to identif...

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Autores principales:	Virginia M. Artegoitia, Andrew P. Foote, Ronald M. Lewis, Harvey C. Freetly
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f568ddbf21ed4373a9b9886e3204e3ba
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spelling	oai:doaj.org-article:f568ddbf21ed4373a9b9886e3204e3ba2021-12-02T16:06:10ZRumen Fluid Metabolomics Analysis Associated with Feed Efficiency on Crossbred Steers10.1038/s41598-017-02856-02045-2322https://doaj.org/article/f568ddbf21ed4373a9b9886e3204e3ba2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02856-0https://doaj.org/toc/2045-2322Abstract The rumen has a central role in the efficiency of digestion in ruminants. To identify potential differences in rumen function that lead to differences in average daily gain (ADG), rumen fluid metabolomic analysis by LC-MS and multivariate/univariate statistical analysis were used to identify differences in rumen metabolites. Individual feed intake and body-weight was measured on 144 steers during 105 d on a high concentrate ration. Eight steers with the greatest ADG and 8 steers with the least-ADG with dry matter intake near the population average were selected. Blood and rumen fluid was collected from the 16 steers 26 d before slaughter and at slaughter, respectively. As a result of the metabolomics analysis of rumen fluid, 33 metabolites differed between the ADG groups based on t-test, fold changes and partial least square discriminant analysis. These metabolites were primarily involved in linoleic and alpha-linolenic metabolism (impact-value 1.0 and 0.75, respectively; P < 0.05); both pathways were down-regulated in the greatest-ADG compared with least-ADG group. Ruminal biohydrogenation might be associated with the overall animal production. The fatty acids were quantified in rumen and plasma using targeted MS to validate and evaluate the simple combination of metabolites that effectively predict ADG.Virginia M. ArtegoitiaAndrew P. FooteRonald M. LewisHarvey C. FreetlyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Virginia M. Artegoitia Andrew P. Foote Ronald M. Lewis Harvey C. Freetly Rumen Fluid Metabolomics Analysis Associated with Feed Efficiency on Crossbred Steers
description	Abstract The rumen has a central role in the efficiency of digestion in ruminants. To identify potential differences in rumen function that lead to differences in average daily gain (ADG), rumen fluid metabolomic analysis by LC-MS and multivariate/univariate statistical analysis were used to identify differences in rumen metabolites. Individual feed intake and body-weight was measured on 144 steers during 105 d on a high concentrate ration. Eight steers with the greatest ADG and 8 steers with the least-ADG with dry matter intake near the population average were selected. Blood and rumen fluid was collected from the 16 steers 26 d before slaughter and at slaughter, respectively. As a result of the metabolomics analysis of rumen fluid, 33 metabolites differed between the ADG groups based on t-test, fold changes and partial least square discriminant analysis. These metabolites were primarily involved in linoleic and alpha-linolenic metabolism (impact-value 1.0 and 0.75, respectively; P < 0.05); both pathways were down-regulated in the greatest-ADG compared with least-ADG group. Ruminal biohydrogenation might be associated with the overall animal production. The fatty acids were quantified in rumen and plasma using targeted MS to validate and evaluate the simple combination of metabolites that effectively predict ADG.
format	article
author	Virginia M. Artegoitia Andrew P. Foote Ronald M. Lewis Harvey C. Freetly
author_facet	Virginia M. Artegoitia Andrew P. Foote Ronald M. Lewis Harvey C. Freetly
author_sort	Virginia M. Artegoitia
title	Rumen Fluid Metabolomics Analysis Associated with Feed Efficiency on Crossbred Steers
title_short	Rumen Fluid Metabolomics Analysis Associated with Feed Efficiency on Crossbred Steers
title_full	Rumen Fluid Metabolomics Analysis Associated with Feed Efficiency on Crossbred Steers
title_fullStr	Rumen Fluid Metabolomics Analysis Associated with Feed Efficiency on Crossbred Steers
title_full_unstemmed	Rumen Fluid Metabolomics Analysis Associated with Feed Efficiency on Crossbred Steers
title_sort	rumen fluid metabolomics analysis associated with feed efficiency on crossbred steers
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f568ddbf21ed4373a9b9886e3204e3ba
work_keys_str_mv	AT virginiamartegoitia rumenfluidmetabolomicsanalysisassociatedwithfeedefficiencyoncrossbredsteers AT andrewpfoote rumenfluidmetabolomicsanalysisassociatedwithfeedefficiencyoncrossbredsteers AT ronaldmlewis rumenfluidmetabolomicsanalysisassociatedwithfeedefficiencyoncrossbredsteers AT harveycfreetly rumenfluidmetabolomicsanalysisassociatedwithfeedefficiencyoncrossbredsteers
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Rumen Fluid Metabolomics Analysis Associated with Feed Efficiency on Crossbred Steers

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