Epitopes identified in GAPDH from Clostridium difficile recognized as common antigens with potential autoimmunizing properties
Abstract Clostridium difficile (CD) infections are a growing threat due to the strain resistance to antibiotic treatment and the emergence of hypervirulent strains. One solution to this problem is the search for new vaccine antigens, preferably surface-localized that will be recognized by antibodies...
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Nature Portfolio
2018
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oai:doaj.org-article:f56b415bf7794a03a67c45f11ba6a61d2021-12-02T15:07:44ZEpitopes identified in GAPDH from Clostridium difficile recognized as common antigens with potential autoimmunizing properties10.1038/s41598-018-32193-92045-2322https://doaj.org/article/f56b415bf7794a03a67c45f11ba6a61d2018-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-32193-9https://doaj.org/toc/2045-2322Abstract Clostridium difficile (CD) infections are a growing threat due to the strain resistance to antibiotic treatment and the emergence of hypervirulent strains. One solution to this problem is the search for new vaccine antigens, preferably surface-localized that will be recognized by antibodies at an early stage of colonization. The purpose of the study was to assess the usefulness of novel immunoreactive surface proteins (epitopes) as potential vaccine antigens. Such approach might be tough to pursue since pathogens have acquired strategies to subvert adaptive immune response to produce humoral response against non-essential proteins for their survival. In this study CD surface proteins were isolated, immunoreactive proteins identified and mapped to select potential epitopes. The results of the study exclude the use of CD glyceraldehyde 3-phosphate dehydrogenase as a vaccine antigen, especially as a whole protein. Sequences P9 (201AAGNIVPNTTGAAKAI218) and P10 (224KGKLDGAAQRVPVVTG241) recognized by patients sera are conserved and widespread among CD strains. They show cross-reactivity with sera of people suffering from other bacterial infections and are recognized by sera of autoimmune disease patients. Our study documents that special care in analyzing the sequence of new epitope should be taken to avoid side effects prior to consider it as a vaccine antigen.Agnieszka RazimKatarzyna PacygaMałgorzata AptekorzGayane MartirosianAndrzej SzubaEdyta Pawlak-AdamskaMonika Brzychczy-WłochAndrzej MycAndrzej GamianSabina GórskaNature PortfolioarticleVaccine AntigensMoonlighting ProteinsGAPDH SequenceUnchanged Amino AcidImmune Epitope DatabaseMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018) |
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Vaccine Antigens Moonlighting Proteins GAPDH Sequence Unchanged Amino Acid Immune Epitope Database Medicine R Science Q |
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Vaccine Antigens Moonlighting Proteins GAPDH Sequence Unchanged Amino Acid Immune Epitope Database Medicine R Science Q Agnieszka Razim Katarzyna Pacyga Małgorzata Aptekorz Gayane Martirosian Andrzej Szuba Edyta Pawlak-Adamska Monika Brzychczy-Włoch Andrzej Myc Andrzej Gamian Sabina Górska Epitopes identified in GAPDH from Clostridium difficile recognized as common antigens with potential autoimmunizing properties |
| description |
Abstract Clostridium difficile (CD) infections are a growing threat due to the strain resistance to antibiotic treatment and the emergence of hypervirulent strains. One solution to this problem is the search for new vaccine antigens, preferably surface-localized that will be recognized by antibodies at an early stage of colonization. The purpose of the study was to assess the usefulness of novel immunoreactive surface proteins (epitopes) as potential vaccine antigens. Such approach might be tough to pursue since pathogens have acquired strategies to subvert adaptive immune response to produce humoral response against non-essential proteins for their survival. In this study CD surface proteins were isolated, immunoreactive proteins identified and mapped to select potential epitopes. The results of the study exclude the use of CD glyceraldehyde 3-phosphate dehydrogenase as a vaccine antigen, especially as a whole protein. Sequences P9 (201AAGNIVPNTTGAAKAI218) and P10 (224KGKLDGAAQRVPVVTG241) recognized by patients sera are conserved and widespread among CD strains. They show cross-reactivity with sera of people suffering from other bacterial infections and are recognized by sera of autoimmune disease patients. Our study documents that special care in analyzing the sequence of new epitope should be taken to avoid side effects prior to consider it as a vaccine antigen. |
| format |
article |
| author |
Agnieszka Razim Katarzyna Pacyga Małgorzata Aptekorz Gayane Martirosian Andrzej Szuba Edyta Pawlak-Adamska Monika Brzychczy-Włoch Andrzej Myc Andrzej Gamian Sabina Górska |
| author_facet |
Agnieszka Razim Katarzyna Pacyga Małgorzata Aptekorz Gayane Martirosian Andrzej Szuba Edyta Pawlak-Adamska Monika Brzychczy-Włoch Andrzej Myc Andrzej Gamian Sabina Górska |
| author_sort |
Agnieszka Razim |
| title |
Epitopes identified in GAPDH from Clostridium difficile recognized as common antigens with potential autoimmunizing properties |
| title_short |
Epitopes identified in GAPDH from Clostridium difficile recognized as common antigens with potential autoimmunizing properties |
| title_full |
Epitopes identified in GAPDH from Clostridium difficile recognized as common antigens with potential autoimmunizing properties |
| title_fullStr |
Epitopes identified in GAPDH from Clostridium difficile recognized as common antigens with potential autoimmunizing properties |
| title_full_unstemmed |
Epitopes identified in GAPDH from Clostridium difficile recognized as common antigens with potential autoimmunizing properties |
| title_sort |
epitopes identified in gapdh from clostridium difficile recognized as common antigens with potential autoimmunizing properties |
| publisher |
Nature Portfolio |
| publishDate |
2018 |
| url |
https://doaj.org/article/f56b415bf7794a03a67c45f11ba6a61d |
| work_keys_str_mv |
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