DAF-18/PTEN inhibits germline zygotic gene activation during primordial germ cell quiescence.

Quiescence, an actively-maintained reversible state of cell cycle arrest, is not well understood. PTEN is one of the most frequently lost tumor suppressors in human cancers and regulates quiescence of stem cells and cancer cells. The sole PTEN ortholog in Caenorhabditis elegans is daf-18. In a C. el...

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Autores principales:	Amanda L Fry, Amy K Webster, Julia Burnett, Rojin Chitrakar, L Ryan Baugh, E Jane Albert Hubbard
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/f571a0900f9e4f4899f901ce5b1ca2e1
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spelling	oai:doaj.org-article:f571a0900f9e4f4899f901ce5b1ca2e12021-12-02T20:02:55ZDAF-18/PTEN inhibits germline zygotic gene activation during primordial germ cell quiescence.1553-73901553-740410.1371/journal.pgen.1009650https://doaj.org/article/f571a0900f9e4f4899f901ce5b1ca2e12021-07-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009650https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Quiescence, an actively-maintained reversible state of cell cycle arrest, is not well understood. PTEN is one of the most frequently lost tumor suppressors in human cancers and regulates quiescence of stem cells and cancer cells. The sole PTEN ortholog in Caenorhabditis elegans is daf-18. In a C. elegans loss-of-function mutant for daf-18, primordial germ cells (PGCs) divide inappropriately in L1 larvae hatched into starvation conditions, in a TOR-dependent manner. Here, we further investigated the role of daf-18 in maintaining PGC quiescence in L1 starvation. We found that maternal or zygotic daf-18 is sufficient to maintain cell cycle quiescence, that daf-18 acts in the germ line and soma, and that daf-18 affects timing of PGC divisions in fed animals. Importantly, our results also implicate daf-18 in repression of germline zygotic gene activation, though not in germline fate specification. However, TOR is less important to germline zygotic gene expression, suggesting that in the absence of food, daf-18/PTEN prevents inappropriate germline zygotic gene activation and cell division by distinct mechanisms.Amanda L FryAmy K WebsterJulia BurnettRojin ChitrakarL Ryan BaughE Jane Albert HubbardPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 7, p e1009650 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Amanda L Fry Amy K Webster Julia Burnett Rojin Chitrakar L Ryan Baugh E Jane Albert Hubbard DAF-18/PTEN inhibits germline zygotic gene activation during primordial germ cell quiescence.
description	Quiescence, an actively-maintained reversible state of cell cycle arrest, is not well understood. PTEN is one of the most frequently lost tumor suppressors in human cancers and regulates quiescence of stem cells and cancer cells. The sole PTEN ortholog in Caenorhabditis elegans is daf-18. In a C. elegans loss-of-function mutant for daf-18, primordial germ cells (PGCs) divide inappropriately in L1 larvae hatched into starvation conditions, in a TOR-dependent manner. Here, we further investigated the role of daf-18 in maintaining PGC quiescence in L1 starvation. We found that maternal or zygotic daf-18 is sufficient to maintain cell cycle quiescence, that daf-18 acts in the germ line and soma, and that daf-18 affects timing of PGC divisions in fed animals. Importantly, our results also implicate daf-18 in repression of germline zygotic gene activation, though not in germline fate specification. However, TOR is less important to germline zygotic gene expression, suggesting that in the absence of food, daf-18/PTEN prevents inappropriate germline zygotic gene activation and cell division by distinct mechanisms.
format	article
author	Amanda L Fry Amy K Webster Julia Burnett Rojin Chitrakar L Ryan Baugh E Jane Albert Hubbard
author_facet	Amanda L Fry Amy K Webster Julia Burnett Rojin Chitrakar L Ryan Baugh E Jane Albert Hubbard
author_sort	Amanda L Fry
title	DAF-18/PTEN inhibits germline zygotic gene activation during primordial germ cell quiescence.
title_short	DAF-18/PTEN inhibits germline zygotic gene activation during primordial germ cell quiescence.
title_full	DAF-18/PTEN inhibits germline zygotic gene activation during primordial germ cell quiescence.
title_fullStr	DAF-18/PTEN inhibits germline zygotic gene activation during primordial germ cell quiescence.
title_full_unstemmed	DAF-18/PTEN inhibits germline zygotic gene activation during primordial germ cell quiescence.
title_sort	daf-18/pten inhibits germline zygotic gene activation during primordial germ cell quiescence.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/f571a0900f9e4f4899f901ce5b1ca2e1
work_keys_str_mv	AT amandalfry daf18pteninhibitsgermlinezygoticgeneactivationduringprimordialgermcellquiescence AT amykwebster daf18pteninhibitsgermlinezygoticgeneactivationduringprimordialgermcellquiescence AT juliaburnett daf18pteninhibitsgermlinezygoticgeneactivationduringprimordialgermcellquiescence AT rojinchitrakar daf18pteninhibitsgermlinezygoticgeneactivationduringprimordialgermcellquiescence AT lryanbaugh daf18pteninhibitsgermlinezygoticgeneactivationduringprimordialgermcellquiescence AT ejanealberthubbard daf18pteninhibitsgermlinezygoticgeneactivationduringprimordialgermcellquiescence
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DAF-18/PTEN inhibits germline zygotic gene activation during primordial germ cell quiescence.

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