One-pot synthesis of dextran-coated iron oxide nanoclusters for real-time regional lymph node mapping

Chaoping Fu,1,* Haipeng Zhou,2,* Yanan Wang,2,* Dong Liu,2 Junmeng Li,2 Haijun Deng,2 Xiaolong Qi,2 Tao Chen,2 Li-Ming Zhang,1 Guoxin Li2 1PCFM Lab and GDHPPC Lab, School of Materials Science and Engineering, Sun Yat-sen University, 2Department of General Surgery, Nanfang Hospital, Southern Medical...

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Autores principales:	Fu C, Zhou H, Wang Y, Liu D, Li J, Deng H, Qi X, Chen T, Zhang LM, Li G
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Dextran-coated iron oxide nanoclusters Lymph node mapping Biosafety Lymphatic endothelial cells Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f57b73fa7892434b8b99b8bc8b218177
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spelling	oai:doaj.org-article:f57b73fa7892434b8b99b8bc8b2181772021-12-02T02:42:05ZOne-pot synthesis of dextran-coated iron oxide nanoclusters for real-time regional lymph node mapping1178-2013https://doaj.org/article/f57b73fa7892434b8b99b8bc8b2181772017-04-01T00:00:00Zhttps://www.dovepress.com/one-pot-synthesis-of-dextran-coated-iron-oxide-nanoclusters-for-real-t-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Chaoping Fu,1,* Haipeng Zhou,2,* Yanan Wang,2,* Dong Liu,2 Junmeng Li,2 Haijun Deng,2 Xiaolong Qi,2 Tao Chen,2 Li-Ming Zhang,1 Guoxin Li2 1PCFM Lab and GDHPPC Lab, School of Materials Science and Engineering, Sun Yat-sen University, 2Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, People’s Republic of China *These authors contributed equally to this work Abstract: The intraoperative precision cleaning of lymph nodes (LNs) is an essential component of treating neoplastic disease. To develop efficient probes for the targeted detection of LNs that could act as carriers for the specific diagnosis and treatment of metastatic LNs in the future, dextran-coated iron oxide nanoclusters (DIONs) were synthesized using a one-pot coprecipitation procedure. These modified DIONs have good water dispersibility, cytocompatibility, an optimum size, and a stable, dark brown color for LN imaging. In this study, cytotoxicity was evaluated using lymphatic endothelial cells (LECs) to predict biosafety and biocompatibility. Most importantly, the effectiveness of DIONs in mapping perigastric LNs in Sprague Dawley rats following injection into the gastric submucosal layer was demonstrated. In addition, a long-term tracing in vivo (from 4 days to 3 months) indicated that the DIONs had good biosafety and biocompatibility according to an evaluation of the behavior and blood biochemistry of the rat and a histopathological examination of the important organs. Keywords: dextran-coated iron oxide nanoclusters, lymph node mapping, biosafety, lymphatic endothelial cellsFu CZhou HWang YLiu DLi JDeng HQi XChen TZhang LMLi GDove Medical PressarticleDextran-coated iron oxide nanoclusters Lymph node mapping Biosafety Lymphatic endothelial cellsMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 3365-3374 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Dextran-coated iron oxide nanoclusters Lymph node mapping Biosafety Lymphatic endothelial cells Medicine (General) R5-920
spellingShingle	Dextran-coated iron oxide nanoclusters Lymph node mapping Biosafety Lymphatic endothelial cells Medicine (General) R5-920 Fu C Zhou H Wang Y Liu D Li J Deng H Qi X Chen T Zhang LM Li G One-pot synthesis of dextran-coated iron oxide nanoclusters for real-time regional lymph node mapping
description	Chaoping Fu,1,* Haipeng Zhou,2,* Yanan Wang,2,* Dong Liu,2 Junmeng Li,2 Haijun Deng,2 Xiaolong Qi,2 Tao Chen,2 Li-Ming Zhang,1 Guoxin Li2 1PCFM Lab and GDHPPC Lab, School of Materials Science and Engineering, Sun Yat-sen University, 2Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, People’s Republic of China *These authors contributed equally to this work Abstract: The intraoperative precision cleaning of lymph nodes (LNs) is an essential component of treating neoplastic disease. To develop efficient probes for the targeted detection of LNs that could act as carriers for the specific diagnosis and treatment of metastatic LNs in the future, dextran-coated iron oxide nanoclusters (DIONs) were synthesized using a one-pot coprecipitation procedure. These modified DIONs have good water dispersibility, cytocompatibility, an optimum size, and a stable, dark brown color for LN imaging. In this study, cytotoxicity was evaluated using lymphatic endothelial cells (LECs) to predict biosafety and biocompatibility. Most importantly, the effectiveness of DIONs in mapping perigastric LNs in Sprague Dawley rats following injection into the gastric submucosal layer was demonstrated. In addition, a long-term tracing in vivo (from 4 days to 3 months) indicated that the DIONs had good biosafety and biocompatibility according to an evaluation of the behavior and blood biochemistry of the rat and a histopathological examination of the important organs. Keywords: dextran-coated iron oxide nanoclusters, lymph node mapping, biosafety, lymphatic endothelial cells
format	article
author	Fu C Zhou H Wang Y Liu D Li J Deng H Qi X Chen T Zhang LM Li G
author_facet	Fu C Zhou H Wang Y Liu D Li J Deng H Qi X Chen T Zhang LM Li G
author_sort	Fu C
title	One-pot synthesis of dextran-coated iron oxide nanoclusters for real-time regional lymph node mapping
title_short	One-pot synthesis of dextran-coated iron oxide nanoclusters for real-time regional lymph node mapping
title_full	One-pot synthesis of dextran-coated iron oxide nanoclusters for real-time regional lymph node mapping
title_fullStr	One-pot synthesis of dextran-coated iron oxide nanoclusters for real-time regional lymph node mapping
title_full_unstemmed	One-pot synthesis of dextran-coated iron oxide nanoclusters for real-time regional lymph node mapping
title_sort	one-pot synthesis of dextran-coated iron oxide nanoclusters for real-time regional lymph node mapping
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/f57b73fa7892434b8b99b8bc8b218177
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One-pot synthesis of dextran-coated iron oxide nanoclusters for real-time regional lymph node mapping

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