Protective activity of the CnaBE3 domain conserved among Staphylococcus aureus Sdr proteins.

Staphylococcus aureus is an opportunistic pathogen, commensal of the human skin and nares, but also responsible for invasive nosocomial as well as community acquired infections. Staphylococcus aureus adheres to the host tissues by means of surface adhesins, such as SdrC, SdrD, and SdrE proteins. The...

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Autores principales:	Marco Becherelli, Prachi Prachi, Elisa Viciani, Massimiliano Biagini, Luigi Fiaschi, Emiliano Chiarot, Sarah Nosari, Cecilia Brettoni, Sara Marchi, Marco Biancucci, Maria Rita Fontana, Francesca Montagnani, Fabio Bagnoli, Michèle A Barocchi, Andrea G O Manetti
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f5829010c3c34dde969e68d4f7c2ec6a
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spelling	oai:doaj.org-article:f5829010c3c34dde969e68d4f7c2ec6a2021-11-18T08:54:54ZProtective activity of the CnaBE3 domain conserved among Staphylococcus aureus Sdr proteins.1932-620310.1371/journal.pone.0074718https://doaj.org/article/f5829010c3c34dde969e68d4f7c2ec6a2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24069334/?tool=EBIhttps://doaj.org/toc/1932-6203Staphylococcus aureus is an opportunistic pathogen, commensal of the human skin and nares, but also responsible for invasive nosocomial as well as community acquired infections. Staphylococcus aureus adheres to the host tissues by means of surface adhesins, such as SdrC, SdrD, and SdrE proteins. The Sdr family of proteins together with a functional A domain, contain respectively two, three or five repeated sequences called B motifs which comprise the CnaB domains. SdrD and SdrE proteins were reported to be protective in animal models against invasive diseases or lethal challenge with human clinical S. aureus isolates. In this study we identified a 126 amino acid sequence containing a CnaB domain, conserved among the three Sdr proteins. The three fragments defined here as CnaBC2, D5 and E3 domains even though belonging to phylogenetically distinct strains, displayed high sequence similarity. Based on the sequence conservation data, we selected the CnaBE3 domain for further analysis and characterization. Polyclonal antibodies raised against the recombinant CnaBE3 domain recognized SdrE, SdrC and SdrD proteins of different S. aureus lineages. Moreover, we demonstrated that the CnaBE3 domain was expressed in vivo during S. aureus infections, and that immunization of this domain alone significantly reduces the bacterial load in mice challenged with S. aureus. Furthermore, we show that the reduction of bacteria by CnaBE3 vaccination is due to functional antibodies. Finally, we demonstrated that the region of the SdrE protein containing the CnaBE3 domain was resistant to trypsin digestion, a characteristic often associated with the presence of an isopeptide bond.Marco BecherelliPrachi PrachiElisa VicianiMassimiliano BiaginiLuigi FiaschiEmiliano ChiarotSarah NosariCecilia BrettoniSara MarchiMarco BiancucciMaria Rita FontanaFrancesca MontagnaniFabio BagnoliMichèle A BarocchiAndrea G O ManettiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e74718 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marco Becherelli Prachi Prachi Elisa Viciani Massimiliano Biagini Luigi Fiaschi Emiliano Chiarot Sarah Nosari Cecilia Brettoni Sara Marchi Marco Biancucci Maria Rita Fontana Francesca Montagnani Fabio Bagnoli Michèle A Barocchi Andrea G O Manetti Protective activity of the CnaBE3 domain conserved among Staphylococcus aureus Sdr proteins.
description	Staphylococcus aureus is an opportunistic pathogen, commensal of the human skin and nares, but also responsible for invasive nosocomial as well as community acquired infections. Staphylococcus aureus adheres to the host tissues by means of surface adhesins, such as SdrC, SdrD, and SdrE proteins. The Sdr family of proteins together with a functional A domain, contain respectively two, three or five repeated sequences called B motifs which comprise the CnaB domains. SdrD and SdrE proteins were reported to be protective in animal models against invasive diseases or lethal challenge with human clinical S. aureus isolates. In this study we identified a 126 amino acid sequence containing a CnaB domain, conserved among the three Sdr proteins. The three fragments defined here as CnaBC2, D5 and E3 domains even though belonging to phylogenetically distinct strains, displayed high sequence similarity. Based on the sequence conservation data, we selected the CnaBE3 domain for further analysis and characterization. Polyclonal antibodies raised against the recombinant CnaBE3 domain recognized SdrE, SdrC and SdrD proteins of different S. aureus lineages. Moreover, we demonstrated that the CnaBE3 domain was expressed in vivo during S. aureus infections, and that immunization of this domain alone significantly reduces the bacterial load in mice challenged with S. aureus. Furthermore, we show that the reduction of bacteria by CnaBE3 vaccination is due to functional antibodies. Finally, we demonstrated that the region of the SdrE protein containing the CnaBE3 domain was resistant to trypsin digestion, a characteristic often associated with the presence of an isopeptide bond.
format	article
author	Marco Becherelli Prachi Prachi Elisa Viciani Massimiliano Biagini Luigi Fiaschi Emiliano Chiarot Sarah Nosari Cecilia Brettoni Sara Marchi Marco Biancucci Maria Rita Fontana Francesca Montagnani Fabio Bagnoli Michèle A Barocchi Andrea G O Manetti
author_facet	Marco Becherelli Prachi Prachi Elisa Viciani Massimiliano Biagini Luigi Fiaschi Emiliano Chiarot Sarah Nosari Cecilia Brettoni Sara Marchi Marco Biancucci Maria Rita Fontana Francesca Montagnani Fabio Bagnoli Michèle A Barocchi Andrea G O Manetti
author_sort	Marco Becherelli
title	Protective activity of the CnaBE3 domain conserved among Staphylococcus aureus Sdr proteins.
title_short	Protective activity of the CnaBE3 domain conserved among Staphylococcus aureus Sdr proteins.
title_full	Protective activity of the CnaBE3 domain conserved among Staphylococcus aureus Sdr proteins.
title_fullStr	Protective activity of the CnaBE3 domain conserved among Staphylococcus aureus Sdr proteins.
title_full_unstemmed	Protective activity of the CnaBE3 domain conserved among Staphylococcus aureus Sdr proteins.
title_sort	protective activity of the cnabe3 domain conserved among staphylococcus aureus sdr proteins.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/f5829010c3c34dde969e68d4f7c2ec6a
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Protective activity of the CnaBE3 domain conserved among Staphylococcus aureus Sdr proteins.

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