Increased leukotoxin production: Characterization of 100 base pairs within the 530 base pair leukotoxin promoter region of Aggregatibacter actinomycetemcomitans

Abstract Aggregatibacter actinomycetemcomitans leukotoxin (LtxA) is a major virulence factor that kills leukocytes permitting it’s escape from host immune surveillance. A. actinomycetemcomitans strains can produce high or low levels of toxin. Genetic differences reside in the “so called JP2” ltxA pr...

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Autores principales:	Vandana Sampathkumar, Senthil Kumar Velusamy, Dipti Godboley, Daniel H. Fine
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f58538ed61814f1bbf41a9e65401a4f6
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spelling	oai:doaj.org-article:f58538ed61814f1bbf41a9e65401a4f62021-12-02T16:06:49ZIncreased leukotoxin production: Characterization of 100 base pairs within the 530 base pair leukotoxin promoter region of Aggregatibacter actinomycetemcomitans10.1038/s41598-017-01692-62045-2322https://doaj.org/article/f58538ed61814f1bbf41a9e65401a4f62017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01692-6https://doaj.org/toc/2045-2322Abstract Aggregatibacter actinomycetemcomitans leukotoxin (LtxA) is a major virulence factor that kills leukocytes permitting it’s escape from host immune surveillance. A. actinomycetemcomitans strains can produce high or low levels of toxin. Genetic differences reside in the “so called JP2” ltxA promoter region. These hyper-leukotoxin producing strains with the 530 bp deletion have been studied in detail. However, regions contained within the 530 bp deletion that could be responsible for modulation of leukotoxin production have not been defined. Here, we report, for the first time, on regions within the 530 bp that are responsible for high-levels of ltxA expression. We constructed a deletion of 530 bps in a primate isolate of A. actinomycetemcomitans, which produced leukotoxin equivalent to the JP2 strain. We then constructed sequential deletions in regions that span the 530 bps. Results indicated that expression of the ltxA transcript was reduced by a potential transcriptional terminator in promoter region 298 to 397 with a ΔG = −7.9 kcal/mol. We also confirmed previous findings that transcriptional fusion between the orfX region and ltxC increased ltxA expression. In conclusion, we constructed a hyper-leukotoxin producing A. actinomycetemcomitans strain and identified a terminator located in the promoter region extending from 298–397 that alters ltxA expression.Vandana SampathkumarSenthil Kumar VelusamyDipti GodboleyDaniel H. FineNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vandana Sampathkumar Senthil Kumar Velusamy Dipti Godboley Daniel H. Fine Increased leukotoxin production: Characterization of 100 base pairs within the 530 base pair leukotoxin promoter region of Aggregatibacter actinomycetemcomitans
description	Abstract Aggregatibacter actinomycetemcomitans leukotoxin (LtxA) is a major virulence factor that kills leukocytes permitting it’s escape from host immune surveillance. A. actinomycetemcomitans strains can produce high or low levels of toxin. Genetic differences reside in the “so called JP2” ltxA promoter region. These hyper-leukotoxin producing strains with the 530 bp deletion have been studied in detail. However, regions contained within the 530 bp deletion that could be responsible for modulation of leukotoxin production have not been defined. Here, we report, for the first time, on regions within the 530 bp that are responsible for high-levels of ltxA expression. We constructed a deletion of 530 bps in a primate isolate of A. actinomycetemcomitans, which produced leukotoxin equivalent to the JP2 strain. We then constructed sequential deletions in regions that span the 530 bps. Results indicated that expression of the ltxA transcript was reduced by a potential transcriptional terminator in promoter region 298 to 397 with a ΔG = −7.9 kcal/mol. We also confirmed previous findings that transcriptional fusion between the orfX region and ltxC increased ltxA expression. In conclusion, we constructed a hyper-leukotoxin producing A. actinomycetemcomitans strain and identified a terminator located in the promoter region extending from 298–397 that alters ltxA expression.
format	article
author	Vandana Sampathkumar Senthil Kumar Velusamy Dipti Godboley Daniel H. Fine
author_facet	Vandana Sampathkumar Senthil Kumar Velusamy Dipti Godboley Daniel H. Fine
author_sort	Vandana Sampathkumar
title	Increased leukotoxin production: Characterization of 100 base pairs within the 530 base pair leukotoxin promoter region of Aggregatibacter actinomycetemcomitans
title_short	Increased leukotoxin production: Characterization of 100 base pairs within the 530 base pair leukotoxin promoter region of Aggregatibacter actinomycetemcomitans
title_full	Increased leukotoxin production: Characterization of 100 base pairs within the 530 base pair leukotoxin promoter region of Aggregatibacter actinomycetemcomitans
title_fullStr	Increased leukotoxin production: Characterization of 100 base pairs within the 530 base pair leukotoxin promoter region of Aggregatibacter actinomycetemcomitans
title_full_unstemmed	Increased leukotoxin production: Characterization of 100 base pairs within the 530 base pair leukotoxin promoter region of Aggregatibacter actinomycetemcomitans
title_sort	increased leukotoxin production: characterization of 100 base pairs within the 530 base pair leukotoxin promoter region of aggregatibacter actinomycetemcomitans
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f58538ed61814f1bbf41a9e65401a4f6
work_keys_str_mv	AT vandanasampathkumar increasedleukotoxinproductioncharacterizationof100basepairswithinthe530basepairleukotoxinpromoterregionofaggregatibacteractinomycetemcomitans AT senthilkumarvelusamy increasedleukotoxinproductioncharacterizationof100basepairswithinthe530basepairleukotoxinpromoterregionofaggregatibacteractinomycetemcomitans AT diptigodboley increasedleukotoxinproductioncharacterizationof100basepairswithinthe530basepairleukotoxinpromoterregionofaggregatibacteractinomycetemcomitans AT danielhfine increasedleukotoxinproductioncharacterizationof100basepairswithinthe530basepairleukotoxinpromoterregionofaggregatibacteractinomycetemcomitans
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Increased leukotoxin production: Characterization of 100 base pairs within the 530 base pair leukotoxin promoter region of Aggregatibacter actinomycetemcomitans

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