Distinct mechanisms involving diacylglycerol, ceramides, and inflammation underlie insulin resistance in oxidative and glycolytic muscles from high fat-fed rats

Abstract This study investigated whether oxidative and glycolytic rat skeletal muscles respond differently to a high-fat (HF) sucrose-enriched diet with respect to diacylglycerol (DAG) and ceramides accumulation, protein kinase C (PKC) activation, glucose metabolism, and the expression of inflammato...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shailee Jani, Daniel Da Eira, Ishvinder Hadday, George Bikopoulos, Arta Mohasses, Ricardo A. de Pinho, Rolando B. Ceddia
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f588ed45a0f646dcbeda81aa211ad7bb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f588ed45a0f646dcbeda81aa211ad7bb
record_format	dspace
spelling	oai:doaj.org-article:f588ed45a0f646dcbeda81aa211ad7bb2021-12-02T19:16:46ZDistinct mechanisms involving diacylglycerol, ceramides, and inflammation underlie insulin resistance in oxidative and glycolytic muscles from high fat-fed rats10.1038/s41598-021-98819-72045-2322https://doaj.org/article/f588ed45a0f646dcbeda81aa211ad7bb2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98819-7https://doaj.org/toc/2045-2322Abstract This study investigated whether oxidative and glycolytic rat skeletal muscles respond differently to a high-fat (HF) sucrose-enriched diet with respect to diacylglycerol (DAG) and ceramides accumulation, protein kinase C (PKC) activation, glucose metabolism, and the expression of inflammatory genes. HF diet (8 weeks) suppressed insulin-stimulated glycogen synthesis and glucose oxidation in soleus (Sol), extensor digitorum longus (EDL) and epitrochlearis (Epit) muscles. However, DAG and ceramides levels increased in Sol and EDL, but not in Epit muscles of HF-fed rats. Additionally, membrane-bound PKC-delta and PKC-theta increased in Sol and EDL, whereas in Epit muscles both PKC isoforms were reduced by HF diet. In Epit muscles, HF diet also increased the expression of tumor necrosis factor-α (TNF-α) receptors (CD40 and FAS), toll-like receptor 4 (TLR4), and nuclear factor kappa light polypeptide gene enhancer in B cells (NF-kB), whereas in Sol and EDL muscles the expression of these inflammatory genes remained unchanged upon HF feeding. In conclusion, HF diet caused DAG and ceramides accumulation, PKC activation, and the induction of inflammatory pathways in a fiber type-specific manner. These findings help explain why oxidative and glycolytic muscles similarly develop insulin resistance, despite major differences in their metabolic characteristics and responsiveness to dietary lipid abundance.Shailee JaniDaniel Da EiraIshvinder HaddayGeorge BikopoulosArta MohassesRicardo A. de PinhoRolando B. CeddiaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shailee Jani Daniel Da Eira Ishvinder Hadday George Bikopoulos Arta Mohasses Ricardo A. de Pinho Rolando B. Ceddia Distinct mechanisms involving diacylglycerol, ceramides, and inflammation underlie insulin resistance in oxidative and glycolytic muscles from high fat-fed rats
description	Abstract This study investigated whether oxidative and glycolytic rat skeletal muscles respond differently to a high-fat (HF) sucrose-enriched diet with respect to diacylglycerol (DAG) and ceramides accumulation, protein kinase C (PKC) activation, glucose metabolism, and the expression of inflammatory genes. HF diet (8 weeks) suppressed insulin-stimulated glycogen synthesis and glucose oxidation in soleus (Sol), extensor digitorum longus (EDL) and epitrochlearis (Epit) muscles. However, DAG and ceramides levels increased in Sol and EDL, but not in Epit muscles of HF-fed rats. Additionally, membrane-bound PKC-delta and PKC-theta increased in Sol and EDL, whereas in Epit muscles both PKC isoforms were reduced by HF diet. In Epit muscles, HF diet also increased the expression of tumor necrosis factor-α (TNF-α) receptors (CD40 and FAS), toll-like receptor 4 (TLR4), and nuclear factor kappa light polypeptide gene enhancer in B cells (NF-kB), whereas in Sol and EDL muscles the expression of these inflammatory genes remained unchanged upon HF feeding. In conclusion, HF diet caused DAG and ceramides accumulation, PKC activation, and the induction of inflammatory pathways in a fiber type-specific manner. These findings help explain why oxidative and glycolytic muscles similarly develop insulin resistance, despite major differences in their metabolic characteristics and responsiveness to dietary lipid abundance.
format	article
author	Shailee Jani Daniel Da Eira Ishvinder Hadday George Bikopoulos Arta Mohasses Ricardo A. de Pinho Rolando B. Ceddia
author_facet	Shailee Jani Daniel Da Eira Ishvinder Hadday George Bikopoulos Arta Mohasses Ricardo A. de Pinho Rolando B. Ceddia
author_sort	Shailee Jani
title	Distinct mechanisms involving diacylglycerol, ceramides, and inflammation underlie insulin resistance in oxidative and glycolytic muscles from high fat-fed rats
title_short	Distinct mechanisms involving diacylglycerol, ceramides, and inflammation underlie insulin resistance in oxidative and glycolytic muscles from high fat-fed rats
title_full	Distinct mechanisms involving diacylglycerol, ceramides, and inflammation underlie insulin resistance in oxidative and glycolytic muscles from high fat-fed rats
title_fullStr	Distinct mechanisms involving diacylglycerol, ceramides, and inflammation underlie insulin resistance in oxidative and glycolytic muscles from high fat-fed rats
title_full_unstemmed	Distinct mechanisms involving diacylglycerol, ceramides, and inflammation underlie insulin resistance in oxidative and glycolytic muscles from high fat-fed rats
title_sort	distinct mechanisms involving diacylglycerol, ceramides, and inflammation underlie insulin resistance in oxidative and glycolytic muscles from high fat-fed rats
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f588ed45a0f646dcbeda81aa211ad7bb
work_keys_str_mv	AT shaileejani distinctmechanismsinvolvingdiacylglycerolceramidesandinflammationunderlieinsulinresistanceinoxidativeandglycolyticmusclesfromhighfatfedrats AT danieldaeira distinctmechanismsinvolvingdiacylglycerolceramidesandinflammationunderlieinsulinresistanceinoxidativeandglycolyticmusclesfromhighfatfedrats AT ishvinderhadday distinctmechanismsinvolvingdiacylglycerolceramidesandinflammationunderlieinsulinresistanceinoxidativeandglycolyticmusclesfromhighfatfedrats AT georgebikopoulos distinctmechanismsinvolvingdiacylglycerolceramidesandinflammationunderlieinsulinresistanceinoxidativeandglycolyticmusclesfromhighfatfedrats AT artamohasses distinctmechanismsinvolvingdiacylglycerolceramidesandinflammationunderlieinsulinresistanceinoxidativeandglycolyticmusclesfromhighfatfedrats AT ricardoadepinho distinctmechanismsinvolvingdiacylglycerolceramidesandinflammationunderlieinsulinresistanceinoxidativeandglycolyticmusclesfromhighfatfedrats AT rolandobceddia distinctmechanismsinvolvingdiacylglycerolceramidesandinflammationunderlieinsulinresistanceinoxidativeandglycolyticmusclesfromhighfatfedrats
_version_	1718376948277706752

Distinct mechanisms involving diacylglycerol, ceramides, and inflammation underlie insulin resistance in oxidative and glycolytic muscles from high fat-fed rats

Ejemplares similares