Decellularized dermis extracellular matrix alloderm mechanically strengthens biological engineered tunica adventitia-based blood vessels

Abstract The ideal engineered vascular graft would utilize human-derived materials to minimize foreign body response and tissue rejection. Current biological engineered blood vessels (BEBVs) inherently lack the structure required for implantation. We hypothesized that an ECM material would provide t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Bijal Patel, Bryan T. Wonski, Dan M. Saliganan, Ali Rteil, Loay S. Kabbani, Mai T. Lam
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f58c798d1ddb41d187fde00eb1e8f423
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f58c798d1ddb41d187fde00eb1e8f423
record_format	dspace
spelling	oai:doaj.org-article:f58c798d1ddb41d187fde00eb1e8f4232021-12-02T17:51:29ZDecellularized dermis extracellular matrix alloderm mechanically strengthens biological engineered tunica adventitia-based blood vessels10.1038/s41598-021-91005-92045-2322https://doaj.org/article/f58c798d1ddb41d187fde00eb1e8f4232021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91005-9https://doaj.org/toc/2045-2322Abstract The ideal engineered vascular graft would utilize human-derived materials to minimize foreign body response and tissue rejection. Current biological engineered blood vessels (BEBVs) inherently lack the structure required for implantation. We hypothesized that an ECM material would provide the structure needed. Skin dermis ECM is commonly used in reconstructive surgeries, is commercially available and FDA-approved. We evaluated the commercially-available decellularized skin dermis ECM Alloderm for efficacy in providing structure to BEBVs. Alloderm was incorporated into our lab’s unique protocol for generating BEBVs, using fibroblasts to establish the adventitia. To assess structure, tissue mechanics were analyzed. Standard BEBVs without Alloderm exhibited a tensile strength of 67.9 ± 9.78 kPa, whereas Alloderm integrated BEBVs showed a significant increase in strength to 1500 ± 334 kPa. In comparison, native vessel strength is 1430 ± 604 kPa. Burst pressure reached 51.3 ± 2.19 mmHg. Total collagen and fiber maturity were significantly increased due to the presence of the Alloderm material. Vessels cultured for 4 weeks maintained mechanical and structural integrity. Low probability of thrombogenicity was confirmed with a negative platelet adhesion test. Vessels were able to be endothelialized. These results demonstrate the success of Alloderm to provide structure to BEBVs in an effective way.Bijal PatelBryan T. WonskiDan M. SaligananAli RteilLoay S. KabbaniMai T. LamNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bijal Patel Bryan T. Wonski Dan M. Saliganan Ali Rteil Loay S. Kabbani Mai T. Lam Decellularized dermis extracellular matrix alloderm mechanically strengthens biological engineered tunica adventitia-based blood vessels
description	Abstract The ideal engineered vascular graft would utilize human-derived materials to minimize foreign body response and tissue rejection. Current biological engineered blood vessels (BEBVs) inherently lack the structure required for implantation. We hypothesized that an ECM material would provide the structure needed. Skin dermis ECM is commonly used in reconstructive surgeries, is commercially available and FDA-approved. We evaluated the commercially-available decellularized skin dermis ECM Alloderm for efficacy in providing structure to BEBVs. Alloderm was incorporated into our lab’s unique protocol for generating BEBVs, using fibroblasts to establish the adventitia. To assess structure, tissue mechanics were analyzed. Standard BEBVs without Alloderm exhibited a tensile strength of 67.9 ± 9.78 kPa, whereas Alloderm integrated BEBVs showed a significant increase in strength to 1500 ± 334 kPa. In comparison, native vessel strength is 1430 ± 604 kPa. Burst pressure reached 51.3 ± 2.19 mmHg. Total collagen and fiber maturity were significantly increased due to the presence of the Alloderm material. Vessels cultured for 4 weeks maintained mechanical and structural integrity. Low probability of thrombogenicity was confirmed with a negative platelet adhesion test. Vessels were able to be endothelialized. These results demonstrate the success of Alloderm to provide structure to BEBVs in an effective way.
format	article
author	Bijal Patel Bryan T. Wonski Dan M. Saliganan Ali Rteil Loay S. Kabbani Mai T. Lam
author_facet	Bijal Patel Bryan T. Wonski Dan M. Saliganan Ali Rteil Loay S. Kabbani Mai T. Lam
author_sort	Bijal Patel
title	Decellularized dermis extracellular matrix alloderm mechanically strengthens biological engineered tunica adventitia-based blood vessels
title_short	Decellularized dermis extracellular matrix alloderm mechanically strengthens biological engineered tunica adventitia-based blood vessels
title_full	Decellularized dermis extracellular matrix alloderm mechanically strengthens biological engineered tunica adventitia-based blood vessels
title_fullStr	Decellularized dermis extracellular matrix alloderm mechanically strengthens biological engineered tunica adventitia-based blood vessels
title_full_unstemmed	Decellularized dermis extracellular matrix alloderm mechanically strengthens biological engineered tunica adventitia-based blood vessels
title_sort	decellularized dermis extracellular matrix alloderm mechanically strengthens biological engineered tunica adventitia-based blood vessels
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f58c798d1ddb41d187fde00eb1e8f423
work_keys_str_mv	AT bijalpatel decellularizeddermisextracellularmatrixallodermmechanicallystrengthensbiologicalengineeredtunicaadventitiabasedbloodvessels AT bryantwonski decellularizeddermisextracellularmatrixallodermmechanicallystrengthensbiologicalengineeredtunicaadventitiabasedbloodvessels AT danmsaliganan decellularizeddermisextracellularmatrixallodermmechanicallystrengthensbiologicalengineeredtunicaadventitiabasedbloodvessels AT alirteil decellularizeddermisextracellularmatrixallodermmechanicallystrengthensbiologicalengineeredtunicaadventitiabasedbloodvessels AT loayskabbani decellularizeddermisextracellularmatrixallodermmechanicallystrengthensbiologicalengineeredtunicaadventitiabasedbloodvessels AT maitlam decellularizeddermisextracellularmatrixallodermmechanicallystrengthensbiologicalengineeredtunicaadventitiabasedbloodvessels
_version_	1718379263258787840

Decellularized dermis extracellular matrix alloderm mechanically strengthens biological engineered tunica adventitia-based blood vessels

Ejemplares similares