Spleen-resident CD4+ and CD4- CD8α- dendritic cell subsets differ in their ability to prime invariant natural killer T lymphocytes.
One important function of conventional dendritic cells (cDC) is their high capacity to capture, process and present Ag to T lymphocytes. Mouse splenic cDC subtypes, including CD8α(+) and CD8α(-) cDC, are not identical in their Ag presenting and T cell priming functions. Surprisingly, few studies hav...
Guardado en:
| Autores principales: | , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2011
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/f592492316654cba9c521e333a8dc5e3 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:f592492316654cba9c521e333a8dc5e3 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:f592492316654cba9c521e333a8dc5e32021-11-18T07:35:22ZSpleen-resident CD4+ and CD4- CD8α- dendritic cell subsets differ in their ability to prime invariant natural killer T lymphocytes.1932-620310.1371/journal.pone.0026919https://doaj.org/article/f592492316654cba9c521e333a8dc5e32011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22066016/?tool=EBIhttps://doaj.org/toc/1932-6203One important function of conventional dendritic cells (cDC) is their high capacity to capture, process and present Ag to T lymphocytes. Mouse splenic cDC subtypes, including CD8α(+) and CD8α(-) cDC, are not identical in their Ag presenting and T cell priming functions. Surprisingly, few studies have reported functional differences between CD4(-) and CD4(+) CD8α(-) cDC subsets. We show that, when loaded in vitro with OVA peptide or whole protein, and in steady-state conditions, splenic CD4(-) and CD4(+) cDC are equivalent in their capacity to prime and direct CD4(+) and CD8(+) T cell differentiation. In contrast, in response to α-galactosylceramide (α-GalCer), CD4(-) and CD4(+) cDC differentially activate invariant Natural Killer T (iNKT) cells, a population of lipid-reactive non-conventional T lymphocytes. Both cDC subsets equally take up α-GalCer in vitro and in vivo to stimulate the iNKT hybridoma DN32.D3, the activation of which depends solely on TCR triggering. On the other hand, and relative to their CD4(+) counterparts, CD4(-) cDC more efficiently stimulate primary iNKT cells, a phenomenon likely due to differential production of co-factors (including IL-12) by cDC. Our data reveal a novel functional difference between splenic CD4(+) and CD4(-) cDC subsets that may be important in immune responses.Emilie BialeckiElodie Macho FernandezStoyan IvanovChristophe PagetJosette FontaineFabien RodriguezLuc LebeauChristophe EhretBenoit FrischFrançois TrotteinChristelle FaveeuwPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 10, p e26919 (2011) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Emilie Bialecki Elodie Macho Fernandez Stoyan Ivanov Christophe Paget Josette Fontaine Fabien Rodriguez Luc Lebeau Christophe Ehret Benoit Frisch François Trottein Christelle Faveeuw Spleen-resident CD4+ and CD4- CD8α- dendritic cell subsets differ in their ability to prime invariant natural killer T lymphocytes. |
| description |
One important function of conventional dendritic cells (cDC) is their high capacity to capture, process and present Ag to T lymphocytes. Mouse splenic cDC subtypes, including CD8α(+) and CD8α(-) cDC, are not identical in their Ag presenting and T cell priming functions. Surprisingly, few studies have reported functional differences between CD4(-) and CD4(+) CD8α(-) cDC subsets. We show that, when loaded in vitro with OVA peptide or whole protein, and in steady-state conditions, splenic CD4(-) and CD4(+) cDC are equivalent in their capacity to prime and direct CD4(+) and CD8(+) T cell differentiation. In contrast, in response to α-galactosylceramide (α-GalCer), CD4(-) and CD4(+) cDC differentially activate invariant Natural Killer T (iNKT) cells, a population of lipid-reactive non-conventional T lymphocytes. Both cDC subsets equally take up α-GalCer in vitro and in vivo to stimulate the iNKT hybridoma DN32.D3, the activation of which depends solely on TCR triggering. On the other hand, and relative to their CD4(+) counterparts, CD4(-) cDC more efficiently stimulate primary iNKT cells, a phenomenon likely due to differential production of co-factors (including IL-12) by cDC. Our data reveal a novel functional difference between splenic CD4(+) and CD4(-) cDC subsets that may be important in immune responses. |
| format |
article |
| author |
Emilie Bialecki Elodie Macho Fernandez Stoyan Ivanov Christophe Paget Josette Fontaine Fabien Rodriguez Luc Lebeau Christophe Ehret Benoit Frisch François Trottein Christelle Faveeuw |
| author_facet |
Emilie Bialecki Elodie Macho Fernandez Stoyan Ivanov Christophe Paget Josette Fontaine Fabien Rodriguez Luc Lebeau Christophe Ehret Benoit Frisch François Trottein Christelle Faveeuw |
| author_sort |
Emilie Bialecki |
| title |
Spleen-resident CD4+ and CD4- CD8α- dendritic cell subsets differ in their ability to prime invariant natural killer T lymphocytes. |
| title_short |
Spleen-resident CD4+ and CD4- CD8α- dendritic cell subsets differ in their ability to prime invariant natural killer T lymphocytes. |
| title_full |
Spleen-resident CD4+ and CD4- CD8α- dendritic cell subsets differ in their ability to prime invariant natural killer T lymphocytes. |
| title_fullStr |
Spleen-resident CD4+ and CD4- CD8α- dendritic cell subsets differ in their ability to prime invariant natural killer T lymphocytes. |
| title_full_unstemmed |
Spleen-resident CD4+ and CD4- CD8α- dendritic cell subsets differ in their ability to prime invariant natural killer T lymphocytes. |
| title_sort |
spleen-resident cd4+ and cd4- cd8α- dendritic cell subsets differ in their ability to prime invariant natural killer t lymphocytes. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2011 |
| url |
https://doaj.org/article/f592492316654cba9c521e333a8dc5e3 |
| work_keys_str_mv |
AT emiliebialecki spleenresidentcd4andcd4cd8adendriticcellsubsetsdifferintheirabilitytoprimeinvariantnaturalkillertlymphocytes AT elodiemachofernandez spleenresidentcd4andcd4cd8adendriticcellsubsetsdifferintheirabilitytoprimeinvariantnaturalkillertlymphocytes AT stoyanivanov spleenresidentcd4andcd4cd8adendriticcellsubsetsdifferintheirabilitytoprimeinvariantnaturalkillertlymphocytes AT christophepaget spleenresidentcd4andcd4cd8adendriticcellsubsetsdifferintheirabilitytoprimeinvariantnaturalkillertlymphocytes AT josettefontaine spleenresidentcd4andcd4cd8adendriticcellsubsetsdifferintheirabilitytoprimeinvariantnaturalkillertlymphocytes AT fabienrodriguez spleenresidentcd4andcd4cd8adendriticcellsubsetsdifferintheirabilitytoprimeinvariantnaturalkillertlymphocytes AT luclebeau spleenresidentcd4andcd4cd8adendriticcellsubsetsdifferintheirabilitytoprimeinvariantnaturalkillertlymphocytes AT christopheehret spleenresidentcd4andcd4cd8adendriticcellsubsetsdifferintheirabilitytoprimeinvariantnaturalkillertlymphocytes AT benoitfrisch spleenresidentcd4andcd4cd8adendriticcellsubsetsdifferintheirabilitytoprimeinvariantnaturalkillertlymphocytes AT francoistrottein spleenresidentcd4andcd4cd8adendriticcellsubsetsdifferintheirabilitytoprimeinvariantnaturalkillertlymphocytes AT christellefaveeuw spleenresidentcd4andcd4cd8adendriticcellsubsetsdifferintheirabilitytoprimeinvariantnaturalkillertlymphocytes |
| _version_ |
1718423261464756224 |