Development of polymeric–cationic peptide composite nanoparticles, a nanoparticle-in-nanoparticle system for controlled gene delivery

Development of polymeric–cationic peptide composite nanoparticles, a nanoparticle-in-nanoparticle system for controlled gene delivery

Arvind K Jain,1,2 Ashley Massey,1 Helmy Yusuf,1 Denise M McDonald,3 Helen O McCarthy,1 Vicky L Kett1 1School of Pharmacy, Medical Biology Centre, Queen’s University Belfast, Belfast, Northern Ireland, UK, 2Weatherall Institute of Molecular Medicine, MRC Molecular Haematology Unit, Univer...

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Autores principales: Jain AK, Massey A, Yusuf H, McDonald DM, McCarthy HO, Kett VL
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2015
Materias:
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/f5992a9101d54a5caaa969fb0a6d8344
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spelling oai:doaj.org-article:f5992a9101d54a5caaa969fb0a6d83442021-12-02T06:46:24ZDevelopment of polymeric–cationic peptide composite nanoparticles, a nanoparticle-in-nanoparticle system for controlled gene delivery1178-2013https://doaj.org/article/f5992a9101d54a5caaa969fb0a6d83442015-11-01T00:00:00Zhttps://www.dovepress.com/development-of-polymericndashcationic-peptide-composite-nanoparticles--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Arvind K Jain,1,2 Ashley Massey,1 Helmy Yusuf,1 Denise M McDonald,3 Helen O McCarthy,1 Vicky L Kett1 1School of Pharmacy, Medical Biology Centre, Queen’s University Belfast, Belfast, Northern Ireland, UK, 2Weatherall Institute of Molecular Medicine, MRC Molecular Haematology Unit, University of Oxford, John Radcliffe Hospital, Oxford, UK, 3Centre for Vision & Vascular Science, Queen’s University Belfast, Belfast, Northern Ireland, UK Abstract: We report the formulation of novel composite nanoparticles that combine the high transfection efficiency of cationic peptide-DNA nanoparticles with the biocompatibility and prolonged delivery of polylactic acid–polyethylene glycol (PLA-PEG). The cationic cell-penetrating peptide RALA was used to condense DNA into nanoparticles that were encapsulated within a range of PLA-PEG copolymers. The composite nanoparticles produced exhibited excellent physicochemical properties including size <200 nm and encapsulation efficiency >80%. Images of the composite nanoparticles obtained with a new transmission electron microscopy staining method revealed the peptide-DNA nanoparticles within the PLA-PEG matrix. Varying the copolymers modulated the DNA release rate >6 weeks in vitro. The best formulation was selected and was able to transfect cells while maintaining viability. The effect of transferrin-appended composite nanoparticles was also studied. Thus, we have demonstrated the manufacture of composite nanoparticles for the controlled delivery of DNA. Keywords: PLA-PEG, cationic peptide, gene delivery, composite nanoparticles, DNA, transfectionJain AKMassey AYusuf HMcDonald DMMcCarthy HOKett VLDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 7183-7196 (2015)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Jain AK
Massey A
Yusuf H
McDonald DM
McCarthy HO
Kett VL
Development of polymeric–cationic peptide composite nanoparticles, a nanoparticle-in-nanoparticle system for controlled gene delivery
description Arvind K Jain,1,2 Ashley Massey,1 Helmy Yusuf,1 Denise M McDonald,3 Helen O McCarthy,1 Vicky L Kett1 1School of Pharmacy, Medical Biology Centre, Queen’s University Belfast, Belfast, Northern Ireland, UK, 2Weatherall Institute of Molecular Medicine, MRC Molecular Haematology Unit, University of Oxford, John Radcliffe Hospital, Oxford, UK, 3Centre for Vision & Vascular Science, Queen’s University Belfast, Belfast, Northern Ireland, UK Abstract: We report the formulation of novel composite nanoparticles that combine the high transfection efficiency of cationic peptide-DNA nanoparticles with the biocompatibility and prolonged delivery of polylactic acid–polyethylene glycol (PLA-PEG). The cationic cell-penetrating peptide RALA was used to condense DNA into nanoparticles that were encapsulated within a range of PLA-PEG copolymers. The composite nanoparticles produced exhibited excellent physicochemical properties including size <200 nm and encapsulation efficiency >80%. Images of the composite nanoparticles obtained with a new transmission electron microscopy staining method revealed the peptide-DNA nanoparticles within the PLA-PEG matrix. Varying the copolymers modulated the DNA release rate >6 weeks in vitro. The best formulation was selected and was able to transfect cells while maintaining viability. The effect of transferrin-appended composite nanoparticles was also studied. Thus, we have demonstrated the manufacture of composite nanoparticles for the controlled delivery of DNA. Keywords: PLA-PEG, cationic peptide, gene delivery, composite nanoparticles, DNA, transfection
format article
author Jain AK
Massey A
Yusuf H
McDonald DM
McCarthy HO
Kett VL
author_facet Jain AK
Massey A
Yusuf H
McDonald DM
McCarthy HO
Kett VL
author_sort Jain AK
title Development of polymeric–cationic peptide composite nanoparticles, a nanoparticle-in-nanoparticle system for controlled gene delivery
title_short Development of polymeric–cationic peptide composite nanoparticles, a nanoparticle-in-nanoparticle system for controlled gene delivery
title_full Development of polymeric–cationic peptide composite nanoparticles, a nanoparticle-in-nanoparticle system for controlled gene delivery
title_fullStr Development of polymeric–cationic peptide composite nanoparticles, a nanoparticle-in-nanoparticle system for controlled gene delivery
title_full_unstemmed Development of polymeric–cationic peptide composite nanoparticles, a nanoparticle-in-nanoparticle system for controlled gene delivery
title_sort development of polymeric–cationic peptide composite nanoparticles, a nanoparticle-in-nanoparticle system for controlled gene delivery
publisher Dove Medical Press
publishDate 2015
url https://doaj.org/article/f5992a9101d54a5caaa969fb0a6d8344
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