Printed elastic membranes for multimodal pacing and recording of human stem-cell-derived cardiomyocytes

Abstract Bioelectronic interfaces employing arrays of sensors and bioactuators are promising tools for the study, repair and engineering of cardiac tissues. They are typically constructed from rigid and brittle materials processed in a cleanroom environment. An outstanding technological challenge is...

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Autores principales:	Markos Athanasiadis, Dzmitry Afanasenkau, Wouter Derks, Christoph Tondera, Francesca Murganti, Volker Busskamp, Olaf Bergmann, Ivan R. Minev
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Electronics TK7800-8360 Materials of engineering and construction. Mechanics of materials TA401-492
Acceso en línea:	https://doaj.org/article/f5a489fb4fb4472e8e32a09e508b0289
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spelling	oai:doaj.org-article:f5a489fb4fb4472e8e32a09e508b02892021-12-02T17:01:16ZPrinted elastic membranes for multimodal pacing and recording of human stem-cell-derived cardiomyocytes10.1038/s41528-020-0075-z2397-4621https://doaj.org/article/f5a489fb4fb4472e8e32a09e508b02892020-07-01T00:00:00Zhttps://doi.org/10.1038/s41528-020-0075-zhttps://doaj.org/toc/2397-4621Abstract Bioelectronic interfaces employing arrays of sensors and bioactuators are promising tools for the study, repair and engineering of cardiac tissues. They are typically constructed from rigid and brittle materials processed in a cleanroom environment. An outstanding technological challenge is the integration of soft materials enabling a closer match to the mechanical properties of biological cells and tissues. Here we present an algorithm for direct writing of elastic membranes with embedded electrodes, optical waveguides and microfluidics using a commercial 3D printing system and a palette of silicone elastomers. As proof of principle, we demonstrate interfacing of cardiomyocytes derived from human induced pluripotent stem cells (hiPSCs), which are engineered to express Channelrhodopsin-2. We demonstrate electrical recording of cardiomyocyte field potentials and their concomitant modulation by optical and pharmacological stimulation delivered via the membrane. Our work contributes a simple prototyping strategy with potential applications in organ-on-chip or implantable systems that are multi-modal and mechanically soft.Markos AthanasiadisDzmitry AfanasenkauWouter DerksChristoph TonderaFrancesca MurgantiVolker BusskampOlaf BergmannIvan R. MinevNature PortfolioarticleElectronicsTK7800-8360Materials of engineering and construction. Mechanics of materialsTA401-492ENnpj Flexible Electronics, Vol 4, Iss 1, Pp 1-8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Electronics TK7800-8360 Materials of engineering and construction. Mechanics of materials TA401-492
spellingShingle	Electronics TK7800-8360 Materials of engineering and construction. Mechanics of materials TA401-492 Markos Athanasiadis Dzmitry Afanasenkau Wouter Derks Christoph Tondera Francesca Murganti Volker Busskamp Olaf Bergmann Ivan R. Minev Printed elastic membranes for multimodal pacing and recording of human stem-cell-derived cardiomyocytes
description	Abstract Bioelectronic interfaces employing arrays of sensors and bioactuators are promising tools for the study, repair and engineering of cardiac tissues. They are typically constructed from rigid and brittle materials processed in a cleanroom environment. An outstanding technological challenge is the integration of soft materials enabling a closer match to the mechanical properties of biological cells and tissues. Here we present an algorithm for direct writing of elastic membranes with embedded electrodes, optical waveguides and microfluidics using a commercial 3D printing system and a palette of silicone elastomers. As proof of principle, we demonstrate interfacing of cardiomyocytes derived from human induced pluripotent stem cells (hiPSCs), which are engineered to express Channelrhodopsin-2. We demonstrate electrical recording of cardiomyocyte field potentials and their concomitant modulation by optical and pharmacological stimulation delivered via the membrane. Our work contributes a simple prototyping strategy with potential applications in organ-on-chip or implantable systems that are multi-modal and mechanically soft.
format	article
author	Markos Athanasiadis Dzmitry Afanasenkau Wouter Derks Christoph Tondera Francesca Murganti Volker Busskamp Olaf Bergmann Ivan R. Minev
author_facet	Markos Athanasiadis Dzmitry Afanasenkau Wouter Derks Christoph Tondera Francesca Murganti Volker Busskamp Olaf Bergmann Ivan R. Minev
author_sort	Markos Athanasiadis
title	Printed elastic membranes for multimodal pacing and recording of human stem-cell-derived cardiomyocytes
title_short	Printed elastic membranes for multimodal pacing and recording of human stem-cell-derived cardiomyocytes
title_full	Printed elastic membranes for multimodal pacing and recording of human stem-cell-derived cardiomyocytes
title_fullStr	Printed elastic membranes for multimodal pacing and recording of human stem-cell-derived cardiomyocytes
title_full_unstemmed	Printed elastic membranes for multimodal pacing and recording of human stem-cell-derived cardiomyocytes
title_sort	printed elastic membranes for multimodal pacing and recording of human stem-cell-derived cardiomyocytes
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/f5a489fb4fb4472e8e32a09e508b0289
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Printed elastic membranes for multimodal pacing and recording of human stem-cell-derived cardiomyocytes

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