Association of decreased triadin expression level with apoptosis of dopaminergic cells in Parkinson’s disease mouse model

Abstract Background Parkinson’s disease (PD) represent a loss of dopaminergic neurons in the substantia nigra (SN) of the midbrain. However, its cause remains unknown and Triadin (TRDN) function in the brain is also unknown. To examine the relationship between TRDN and PD, the expression levels of p...

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Autores principales:	Min Hyung Seo, Sabina Lim, Sujung Yeo
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	TRDN Parkinson’s disease Apoptosis MPTP SH-SY5Y Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurophysiology and neuropsychology QP351-495
Acceso en línea:	https://doaj.org/article/f5a9477a4ef94e7d928249013711a13f
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spelling	oai:doaj.org-article:f5a9477a4ef94e7d928249013711a13f2021-11-08T11:05:13ZAssociation of decreased triadin expression level with apoptosis of dopaminergic cells in Parkinson’s disease mouse model10.1186/s12868-021-00668-71471-2202https://doaj.org/article/f5a9477a4ef94e7d928249013711a13f2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12868-021-00668-7https://doaj.org/toc/1471-2202Abstract Background Parkinson’s disease (PD) represent a loss of dopaminergic neurons in the substantia nigra (SN) of the midbrain. However, its cause remains unknown and Triadin (TRDN) function in the brain is also unknown. To examine the relationship between TRDN and PD, the expression levels of protein related to PD in TRDN knockdown status were studied in the SH-SY5Y cells. Cell viability and apoptosis were assessed to examine the apoptosis effect on dopaminergic cells by decreased TRDN, and the levels of the proteins related to apoptosis were also confirmed. Results This study confirmed decreased TRDN expression level (P < 0.005) at the SN in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced PD mouse model and identified the functional features of TRDN. Our results showed a relationship between TRDN expression and PD in that reduced TRDN level induced PD-like characteristics. Interestingly, there was TRDN expression in the regions where dopaminergic cells are in the SN, and the expression patterns of TRDN and tyrosine hydroxylase (TH) were similar. Decreased TRDN level also induced apoptotic characteristics and the Fluorescence-activated cell sorting analysis results showed that apoptosis increased (P < 0.05) as the TRDN small interfering RNA concentration increased. The cytotoxicity assay revealed that cell viability also decreased (P < 0.0005) in the same condition as that in the Fluorescence‐activated cell sorting analysis. Conclusions Decreased TRDN level could be related with the apoptotic death of dopaminergic cells at the SN in PD, and TRDN administration could give a positive effect on PD by reducing apoptotic cell death.Min Hyung SeoSabina LimSujung YeoBMCarticleTRDNParkinson’s diseaseApoptosisMPTPSH-SY5YNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurophysiology and neuropsychologyQP351-495ENBMC Neuroscience, Vol 22, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	TRDN Parkinson’s disease Apoptosis MPTP SH-SY5Y Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurophysiology and neuropsychology QP351-495
spellingShingle	TRDN Parkinson’s disease Apoptosis MPTP SH-SY5Y Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurophysiology and neuropsychology QP351-495 Min Hyung Seo Sabina Lim Sujung Yeo Association of decreased triadin expression level with apoptosis of dopaminergic cells in Parkinson’s disease mouse model
description	Abstract Background Parkinson’s disease (PD) represent a loss of dopaminergic neurons in the substantia nigra (SN) of the midbrain. However, its cause remains unknown and Triadin (TRDN) function in the brain is also unknown. To examine the relationship between TRDN and PD, the expression levels of protein related to PD in TRDN knockdown status were studied in the SH-SY5Y cells. Cell viability and apoptosis were assessed to examine the apoptosis effect on dopaminergic cells by decreased TRDN, and the levels of the proteins related to apoptosis were also confirmed. Results This study confirmed decreased TRDN expression level (P < 0.005) at the SN in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced PD mouse model and identified the functional features of TRDN. Our results showed a relationship between TRDN expression and PD in that reduced TRDN level induced PD-like characteristics. Interestingly, there was TRDN expression in the regions where dopaminergic cells are in the SN, and the expression patterns of TRDN and tyrosine hydroxylase (TH) were similar. Decreased TRDN level also induced apoptotic characteristics and the Fluorescence-activated cell sorting analysis results showed that apoptosis increased (P < 0.05) as the TRDN small interfering RNA concentration increased. The cytotoxicity assay revealed that cell viability also decreased (P < 0.0005) in the same condition as that in the Fluorescence‐activated cell sorting analysis. Conclusions Decreased TRDN level could be related with the apoptotic death of dopaminergic cells at the SN in PD, and TRDN administration could give a positive effect on PD by reducing apoptotic cell death.
format	article
author	Min Hyung Seo Sabina Lim Sujung Yeo
author_facet	Min Hyung Seo Sabina Lim Sujung Yeo
author_sort	Min Hyung Seo
title	Association of decreased triadin expression level with apoptosis of dopaminergic cells in Parkinson’s disease mouse model
title_short	Association of decreased triadin expression level with apoptosis of dopaminergic cells in Parkinson’s disease mouse model
title_full	Association of decreased triadin expression level with apoptosis of dopaminergic cells in Parkinson’s disease mouse model
title_fullStr	Association of decreased triadin expression level with apoptosis of dopaminergic cells in Parkinson’s disease mouse model
title_full_unstemmed	Association of decreased triadin expression level with apoptosis of dopaminergic cells in Parkinson’s disease mouse model
title_sort	association of decreased triadin expression level with apoptosis of dopaminergic cells in parkinson’s disease mouse model
publisher	BMC
publishDate	2021
url	https://doaj.org/article/f5a9477a4ef94e7d928249013711a13f
work_keys_str_mv	AT minhyungseo associationofdecreasedtriadinexpressionlevelwithapoptosisofdopaminergiccellsinparkinsonsdiseasemousemodel AT sabinalim associationofdecreasedtriadinexpressionlevelwithapoptosisofdopaminergiccellsinparkinsonsdiseasemousemodel AT sujungyeo associationofdecreasedtriadinexpressionlevelwithapoptosisofdopaminergiccellsinparkinsonsdiseasemousemodel
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Association of decreased triadin expression level with apoptosis of dopaminergic cells in Parkinson’s disease mouse model

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