Size-dependent antimicrobial properties of CuO nanoparticles against Gram-positive and -negative bacterial strains

Ameer Azam,1,2 Arham S Ahmed,2 M Oves,3 MS Khan,3 Adnan Memic11Center of Nanotechnology, King Abdulaziz University, Jeddah, Saudi Arabia; 2Center of Excellence in Materials Science (Nanomaterials), Department of Applied Physics, 3Department of Agricultural Microbiology, Aligarh Muslim University, Al...

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Autores principales:	Azam A, Ahmed AS, Oves M, Khan MS, Memic A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f5ac78872ee440d09c37d8d7a1fa23d5
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spelling	oai:doaj.org-article:f5ac78872ee440d09c37d8d7a1fa23d52021-12-02T02:10:28ZSize-dependent antimicrobial properties of CuO nanoparticles against Gram-positive and -negative bacterial strains1176-91141178-2013https://doaj.org/article/f5ac78872ee440d09c37d8d7a1fa23d52012-07-01T00:00:00Zhttp://www.dovepress.com/size-dependent-antimicrobial-properties-of-cuo-nanoparticles-against-g-a10351https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Ameer Azam,1,2 Arham S Ahmed,2 M Oves,3 MS Khan,3 Adnan Memic11Center of Nanotechnology, King Abdulaziz University, Jeddah, Saudi Arabia; 2Center of Excellence in Materials Science (Nanomaterials), Department of Applied Physics, 3Department of Agricultural Microbiology, Aligarh Muslim University, Aligarh, IndiaBackground: CuO is one of the most important transition metal oxides due to its captivating properties. It is used in various technological applications such as high critical temperature superconductors, gas sensors, in photoconductive applications, and so on. Recently, it has been used as an antimicrobial agent against various bacterial species. Here we synthesized different sized CuO nanoparticles and explored the size-dependent antibacterial activity of each CuO nanoparticles preparation.Methods: CuO nanoparticles were synthesized using a gel combustion method. In this approach, cupric nitrate trihydrate and citric acid were dissolved in distilled water with a molar ratio of 1:1. The resulting solution was stirred at 100&deg;C, until gel was formed. The gel was allowed to burn at 200&deg;C to obtain amorphous powder, which was further annealed at different temperatures to obtain different size CuO nanoparticles. We then tested the antibacterial properties using well diffusion, minimum inhibitory concentration, and minimum bactericidal concentration methods.Results: XRD spectra confirmed the formation of single phase CuO nanoparticles. Crystallite size was found to increase with an increase in annealing temperature due to atomic diffusion. A minimum crystallite size of 20 nm was observed in the case of CuO nanoparticles annealed at 400&deg;C. Transmission electron microscopy results corroborate well with XRD results. All CuO nanoparticles exhibited inhibitory effects against both Gram-positive and -negative bacteria. The size of the particles was correlated with its antibacterial activity.Conclusion: The antibacterial activity of CuO nanoparticles was found to be size-dependent. In addition, the highly stable minimum-sized monodispersed copper oxide nanoparticles synthesized during this study demonstrated a significant increase in antibacterial activities against both Gram-positive and -negative bacterial strains.Keywords: CuO, nanoparticles, X-ray diffraction, FTIR, antimicrobial activityAzam AAhmed ASOves MKhan MSMemic ADove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 3527-3535 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Azam A Ahmed AS Oves M Khan MS Memic A Size-dependent antimicrobial properties of CuO nanoparticles against Gram-positive and -negative bacterial strains
description	Ameer Azam,1,2 Arham S Ahmed,2 M Oves,3 MS Khan,3 Adnan Memic11Center of Nanotechnology, King Abdulaziz University, Jeddah, Saudi Arabia; 2Center of Excellence in Materials Science (Nanomaterials), Department of Applied Physics, 3Department of Agricultural Microbiology, Aligarh Muslim University, Aligarh, IndiaBackground: CuO is one of the most important transition metal oxides due to its captivating properties. It is used in various technological applications such as high critical temperature superconductors, gas sensors, in photoconductive applications, and so on. Recently, it has been used as an antimicrobial agent against various bacterial species. Here we synthesized different sized CuO nanoparticles and explored the size-dependent antibacterial activity of each CuO nanoparticles preparation.Methods: CuO nanoparticles were synthesized using a gel combustion method. In this approach, cupric nitrate trihydrate and citric acid were dissolved in distilled water with a molar ratio of 1:1. The resulting solution was stirred at 100&deg;C, until gel was formed. The gel was allowed to burn at 200&deg;C to obtain amorphous powder, which was further annealed at different temperatures to obtain different size CuO nanoparticles. We then tested the antibacterial properties using well diffusion, minimum inhibitory concentration, and minimum bactericidal concentration methods.Results: XRD spectra confirmed the formation of single phase CuO nanoparticles. Crystallite size was found to increase with an increase in annealing temperature due to atomic diffusion. A minimum crystallite size of 20 nm was observed in the case of CuO nanoparticles annealed at 400&deg;C. Transmission electron microscopy results corroborate well with XRD results. All CuO nanoparticles exhibited inhibitory effects against both Gram-positive and -negative bacteria. The size of the particles was correlated with its antibacterial activity.Conclusion: The antibacterial activity of CuO nanoparticles was found to be size-dependent. In addition, the highly stable minimum-sized monodispersed copper oxide nanoparticles synthesized during this study demonstrated a significant increase in antibacterial activities against both Gram-positive and -negative bacterial strains.Keywords: CuO, nanoparticles, X-ray diffraction, FTIR, antimicrobial activity
format	article
author	Azam A Ahmed AS Oves M Khan MS Memic A
author_facet	Azam A Ahmed AS Oves M Khan MS Memic A
author_sort	Azam A
title	Size-dependent antimicrobial properties of CuO nanoparticles against Gram-positive and -negative bacterial strains
title_short	Size-dependent antimicrobial properties of CuO nanoparticles against Gram-positive and -negative bacterial strains
title_full	Size-dependent antimicrobial properties of CuO nanoparticles against Gram-positive and -negative bacterial strains
title_fullStr	Size-dependent antimicrobial properties of CuO nanoparticles against Gram-positive and -negative bacterial strains
title_full_unstemmed	Size-dependent antimicrobial properties of CuO nanoparticles against Gram-positive and -negative bacterial strains
title_sort	size-dependent antimicrobial properties of cuo nanoparticles against gram-positive and -negative bacterial strains
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/f5ac78872ee440d09c37d8d7a1fa23d5
work_keys_str_mv	AT azama sizedependentantimicrobialpropertiesofcuonanoparticlesagainstgrampositiveandnegativebacterialstrains AT ahmedas sizedependentantimicrobialpropertiesofcuonanoparticlesagainstgrampositiveandnegativebacterialstrains AT ovesm sizedependentantimicrobialpropertiesofcuonanoparticlesagainstgrampositiveandnegativebacterialstrains AT khanms sizedependentantimicrobialpropertiesofcuonanoparticlesagainstgrampositiveandnegativebacterialstrains AT memica sizedependentantimicrobialpropertiesofcuonanoparticlesagainstgrampositiveandnegativebacterialstrains
_version_	1718402687781830656

Size-dependent antimicrobial properties of CuO nanoparticles against Gram-positive and -negative bacterial strains

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