Positive Salt Tolerance Modulation via Vermicompost Regulation of SOS1 Gene Expression and Antioxidant Homeostasis in Viciafaba Plant

Strategic implementation of vermicompost as safe biofertilizer besides defensing saline soils offer dual function solving problems in developing countries. The current study aims to utilize vermicompost (VC) for amelioration of 200mM NaCl in Vicia faba Aspani cultivar and investig...

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Autores principales:	Rehab El-Dakak, Weam El-Aggan, Ghadah Badr, Amira Helaly, Amel Tammam
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Vicia</i> <i>faba</i> broad bean salinity SOS1 stress vermicompost Botany QK1-989
Acceso en línea:	https://doaj.org/article/f5aedb5051574562bcf23a457847af1b
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spelling	oai:doaj.org-article:f5aedb5051574562bcf23a457847af1b2021-11-25T18:46:54ZPositive Salt Tolerance Modulation via Vermicompost Regulation of SOS1 Gene Expression and Antioxidant Homeostasis in <i>Vicia</i><i>faba</i> Plant10.3390/plants101124772223-7747https://doaj.org/article/f5aedb5051574562bcf23a457847af1b2021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2477https://doaj.org/toc/2223-7747Strategic implementation of vermicompost as safe biofertilizer besides defensing saline soils offer dual function solving problems in developing countries. The current study aims to utilize vermicompost (VC) for amelioration of 200mM NaCl in <i>Vicia faba</i> Aspani cultivar and investigate the molecular role of salt overly sensitive pathway (SOS1). The experiment was conducted following a completely randomized design with three replicates. Treatments include 0; 2.5; 5; 10; 15% dried VC intermingled with soil mixture (clay: sand; 1:2) and/or 200 mM NaCl. The results show that salinity stress decreased broad bean fresh and dry weight; and K<sup>+</sup>/Na<sup>+</sup>. However, malonedialdehyde and H<sub>2</sub>O<sub>2</sub> contents; increased. Application of 10% VC and salinity stress increases Ca<sup>2+</sup> (41% and 50%), K<sup>+</sup>/Na<sup>+</sup> (125% and 89%), Mg<sup>2+</sup> (25% and 36%), N (8% and 11%), indole acetic acid (70% and 152%) and proteins (9% and 13%) for root and shoot, respectively, in comparison to salt treated pots. Moreover, all examined enzymatic antioxidants and their substrates increased, except glutathione reductase. A parallel decrease in abscisic acid (75% and 29%) and proline (59% and 58%) was also recorded for roots and leaves, respectively. Interestingly, the highly significant increase in gene expression of SOS1 (45-fold) could drive defense machinery of broad bean to counteract 200 mM NaCl.Rehab El-DakakWeam El-AgganGhadah BadrAmira HelalyAmel TammamMDPI AGarticle<i>Vicia</i> <i>faba</i>broad beansalinitySOS1stressvermicompostBotanyQK1-989ENPlants, Vol 10, Iss 2477, p 2477 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Vicia</i> <i>faba</i> broad bean salinity SOS1 stress vermicompost Botany QK1-989
spellingShingle	<i>Vicia</i> <i>faba</i> broad bean salinity SOS1 stress vermicompost Botany QK1-989 Rehab El-Dakak Weam El-Aggan Ghadah Badr Amira Helaly Amel Tammam Positive Salt Tolerance Modulation via Vermicompost Regulation of SOS1 Gene Expression and Antioxidant Homeostasis in <i>Vicia</i><i>faba</i> Plant
description	Strategic implementation of vermicompost as safe biofertilizer besides defensing saline soils offer dual function solving problems in developing countries. The current study aims to utilize vermicompost (VC) for amelioration of 200mM NaCl in <i>Vicia faba</i> Aspani cultivar and investigate the molecular role of salt overly sensitive pathway (SOS1). The experiment was conducted following a completely randomized design with three replicates. Treatments include 0; 2.5; 5; 10; 15% dried VC intermingled with soil mixture (clay: sand; 1:2) and/or 200 mM NaCl. The results show that salinity stress decreased broad bean fresh and dry weight; and K<sup>+</sup>/Na<sup>+</sup>. However, malonedialdehyde and H<sub>2</sub>O<sub>2</sub> contents; increased. Application of 10% VC and salinity stress increases Ca<sup>2+</sup> (41% and 50%), K<sup>+</sup>/Na<sup>+</sup> (125% and 89%), Mg<sup>2+</sup> (25% and 36%), N (8% and 11%), indole acetic acid (70% and 152%) and proteins (9% and 13%) for root and shoot, respectively, in comparison to salt treated pots. Moreover, all examined enzymatic antioxidants and their substrates increased, except glutathione reductase. A parallel decrease in abscisic acid (75% and 29%) and proline (59% and 58%) was also recorded for roots and leaves, respectively. Interestingly, the highly significant increase in gene expression of SOS1 (45-fold) could drive defense machinery of broad bean to counteract 200 mM NaCl.
format	article
author	Rehab El-Dakak Weam El-Aggan Ghadah Badr Amira Helaly Amel Tammam
author_facet	Rehab El-Dakak Weam El-Aggan Ghadah Badr Amira Helaly Amel Tammam
author_sort	Rehab El-Dakak
title	Positive Salt Tolerance Modulation via Vermicompost Regulation of SOS1 Gene Expression and Antioxidant Homeostasis in <i>Vicia</i><i>faba</i> Plant
title_short	Positive Salt Tolerance Modulation via Vermicompost Regulation of SOS1 Gene Expression and Antioxidant Homeostasis in <i>Vicia</i><i>faba</i> Plant
title_full	Positive Salt Tolerance Modulation via Vermicompost Regulation of SOS1 Gene Expression and Antioxidant Homeostasis in <i>Vicia</i><i>faba</i> Plant
title_fullStr	Positive Salt Tolerance Modulation via Vermicompost Regulation of SOS1 Gene Expression and Antioxidant Homeostasis in <i>Vicia</i><i>faba</i> Plant
title_full_unstemmed	Positive Salt Tolerance Modulation via Vermicompost Regulation of SOS1 Gene Expression and Antioxidant Homeostasis in <i>Vicia</i><i>faba</i> Plant
title_sort	positive salt tolerance modulation via vermicompost regulation of sos1 gene expression and antioxidant homeostasis in <i>vicia</i><i>faba</i> plant
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f5aedb5051574562bcf23a457847af1b
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_version_	1718410749971267584

Positive Salt Tolerance Modulation via Vermicompost Regulation of SOS1 Gene Expression and Antioxidant Homeostasis in <i>Vicia</i><i>faba</i> Plant

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