In vivo genetic manipulation of inner ear connexin expression by bovine adeno-associated viral vectors

Abstract We have previously shown that in vitro transduction with bovine adeno–associated viral (BAAV) vectors restores connexin expression and rescues gap junction coupling in cochlear organotypic cultures from connexin–deficient mice that are models DFNB1 nonsyndromic hearing loss and deafness. Th...

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Autores principales:	Giulia Crispino, Fabian Galindo Ramirez, Matteo Campioni, Veronica Zorzi, Mark Praetorius, Giovanni Di Pasquale, John A. Chiorini, Fabio Mammano
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f5b21be5ccfe4f96b4c632312c5f3207
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spelling	oai:doaj.org-article:f5b21be5ccfe4f96b4c632312c5f32072021-12-02T12:32:14ZIn vivo genetic manipulation of inner ear connexin expression by bovine adeno-associated viral vectors10.1038/s41598-017-06759-y2045-2322https://doaj.org/article/f5b21be5ccfe4f96b4c632312c5f32072017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06759-yhttps://doaj.org/toc/2045-2322Abstract We have previously shown that in vitro transduction with bovine adeno–associated viral (BAAV) vectors restores connexin expression and rescues gap junction coupling in cochlear organotypic cultures from connexin–deficient mice that are models DFNB1 nonsyndromic hearing loss and deafness. The aims of this study were to manipulate inner ear connexin expression in vivo using BAAV vectors, and to identify the optimal route of vector delivery. Injection of a BAAV vector encoding a bacterial Cre recombinase via canalostomy in adult mice with floxed connexin 26 (Cx26) alleles promoted Cre/LoxP recombination, resulting in decreased Cx26 expression, decreased endocochlear potential, increased hearing thresholds, and extensive loss of outer hair cells. Injection of a BAAV vector encoding GFP-tagged Cx30 via canalostomy in P4 mice lacking connexin 30 (Cx30) promoted formation of Cx30 gap junctions at points of contacts between adjacent non-sensory cells of the cochlear sensory epithelium. Levels of exogenous Cx30 decayed over time, but were still detectable four weeks after canalostomy. Our results suggest that persistence of BAAV-mediated gene replacement in the cochlea is limited by the extensive remodeling of the organ of Corti throughout postnatal development and associated loss of non-sensory cells.Giulia CrispinoFabian Galindo RamirezMatteo CampioniVeronica ZorziMark PraetoriusGiovanni Di PasqualeJohn A. ChioriniFabio MammanoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Giulia Crispino Fabian Galindo Ramirez Matteo Campioni Veronica Zorzi Mark Praetorius Giovanni Di Pasquale John A. Chiorini Fabio Mammano In vivo genetic manipulation of inner ear connexin expression by bovine adeno-associated viral vectors
description	Abstract We have previously shown that in vitro transduction with bovine adeno–associated viral (BAAV) vectors restores connexin expression and rescues gap junction coupling in cochlear organotypic cultures from connexin–deficient mice that are models DFNB1 nonsyndromic hearing loss and deafness. The aims of this study were to manipulate inner ear connexin expression in vivo using BAAV vectors, and to identify the optimal route of vector delivery. Injection of a BAAV vector encoding a bacterial Cre recombinase via canalostomy in adult mice with floxed connexin 26 (Cx26) alleles promoted Cre/LoxP recombination, resulting in decreased Cx26 expression, decreased endocochlear potential, increased hearing thresholds, and extensive loss of outer hair cells. Injection of a BAAV vector encoding GFP-tagged Cx30 via canalostomy in P4 mice lacking connexin 30 (Cx30) promoted formation of Cx30 gap junctions at points of contacts between adjacent non-sensory cells of the cochlear sensory epithelium. Levels of exogenous Cx30 decayed over time, but were still detectable four weeks after canalostomy. Our results suggest that persistence of BAAV-mediated gene replacement in the cochlea is limited by the extensive remodeling of the organ of Corti throughout postnatal development and associated loss of non-sensory cells.
format	article
author	Giulia Crispino Fabian Galindo Ramirez Matteo Campioni Veronica Zorzi Mark Praetorius Giovanni Di Pasquale John A. Chiorini Fabio Mammano
author_facet	Giulia Crispino Fabian Galindo Ramirez Matteo Campioni Veronica Zorzi Mark Praetorius Giovanni Di Pasquale John A. Chiorini Fabio Mammano
author_sort	Giulia Crispino
title	In vivo genetic manipulation of inner ear connexin expression by bovine adeno-associated viral vectors
title_short	In vivo genetic manipulation of inner ear connexin expression by bovine adeno-associated viral vectors
title_full	In vivo genetic manipulation of inner ear connexin expression by bovine adeno-associated viral vectors
title_fullStr	In vivo genetic manipulation of inner ear connexin expression by bovine adeno-associated viral vectors
title_full_unstemmed	In vivo genetic manipulation of inner ear connexin expression by bovine adeno-associated viral vectors
title_sort	in vivo genetic manipulation of inner ear connexin expression by bovine adeno-associated viral vectors
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f5b21be5ccfe4f96b4c632312c5f3207
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In vivo genetic manipulation of inner ear connexin expression by bovine adeno-associated viral vectors

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